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Rat Epithelial neutrophil activating peptide 78,ENA-78 ELISA Kit

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Protocol MSDS
Code CSB-E08179r
Size 96T,5×96T,10×96T
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Product Details

Target Name
chemokine (C-X-C motif) ligand 5
Alternative Names
Cxcl5 ELISA Kit; Scyb5C-X-C motif chemokine 5 ELISA Kit; Cytokine LIX ELISA Kit; Small-inducible cytokine B5 ELISA Kit
Abbreviation
CXCL5
Uniprot No.
P97885
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　

Intra-Assay Precision

Inter-Assay Precision

Sample

1

2

3

1

2

3

n

20

20

20

20

20

20

Mean(ng/ml)

2.442

2.474

2.529

2.446

2.487

2.542

SD

0.035

0.033

0.038

0.041

0.051

0.048

CV(%)

5.486

5.116

5.784

6.416

7.870

7.273

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

　

Sample

Serum(n=4)

1:1

Average %

88

Range %

83-91

1:2

Average %

104

Range %

98-108

1:4

Average %

92

Range %

87-96

1:8

Average %

96

Range %

91-99

Recovery

The recovery of rat ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

90

84-94

EDTA plasma (n=4)

94

87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

20

2.746

2.689

2.718

2.607

10

2.000

1.899

1.950

1.839

5

1.190

1.171

1.181

1.070

2.5

0.651

0.640

0.646

0.535

1.25

0.366

0.354

0.360

0.249

0.625

0.263

0.257

0.260

0.149

0.312

0.172

0.178

0.175

0.064

0.064

0

0.110

0.112

0.111

　

　

ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Rat CXCL5 ELISA Kit was designed for the quantitative measurement of Rat CXCL5 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

Related Products

Cxcl5 Proteins

Customer Reviews and Q&A

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Target Background

Function
(From Uniprot)
May participate in the recruitment of inflammatory cells by injured or infected tissue.
Gene References into Functions
  1. CRH suppressed CXCL5 production in glia via CRHR1 in the hippocampus of male rats. PMID: 29126185
  2. up-regulation of spinal CXCL5 and CXCR2 is involved in neuropathic pain after nerve injury, through regulating GSK-3beta activity in rats. PMID: 27717828
  3. Analysis of mitogen-activated protein kinases (MAPK) and glycogen synthase kinase (GSK3) pathways showed that they are involved in mechanisms of neuronal apoptosis in response to the depletion of CXCL5/LIX signaling. PMID: 22034072
  4. Data demonstrate that the chemokine CXCL5 is a peripheral mediator of UVB-induced inflammatory pain, likely in humans as well as rats. PMID: 21734176
  5. Disrupted expression of CXCL5 in colorectal cancer is associated with rapid tumor formation in rats and poor prognosis in patients. PMID: 18413816

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Subcellular Location
Secreted.
Protein Families
Intercrine alpha (chemokine CxC) family
Database Links
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Related pathways
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