Rat Hepcidin(HAMP) ELISA kit

Code CSB-EL010124RA
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Description

This Rat HAMP ELISA Kit was designed for the quantitative measurement of Rat HAMP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL .

Target Name hepcidin antimicrobial peptide
Alternative Names Hamp ELISA Kit; Hepc ELISA Kit; Hepcidin ELISA Kit
Abbreviation HAMP
Uniprot No. Q99MH3
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 84-96  
1:2 Average % 95  
Range % 91-101  
1:4 Average % 84  
Range % 81-89  
1:8 Average % 89  
Range % 85-94  
Recovery
The recovery of rat HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-96  
EDTA plasma (n=4) 96 91-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.262 2.286 2.274 2.123  
5 1.644 1.789 1.717 1.566  
2.5 1.216 1.256 1.236 1.085  
1.25 0.876 0.950 0.913 0.762  
0.625 0.501 0.465 0.483 0.332  
0.312 0.305 0.308 0.307 0.156  
0.156 0.212 0.223 0.218 0.067  
0 0.152 0.150 0.151    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

 Q&A
Q:

Hi, May I know your 'rat hepcidin ELISA kit (CSB-EL010124RA)' either detects mature hepcidin (25, 22 ,20 amino acid) or pro-hepcidin (84 amino acid)?
I'd purchased this kit through Sungwoo LifeScience, and the result was sufficient. I'm going to be even more happy if I know that which form of hepcidin is detected by the kit.
Thank you for supplying many effecient kits.

A:
Thanks for your email and your concern & trust on Cusabio!
The mentioned kit#CSB-EL010124RA is directed to full length.Theoretically,this kit can detect kinds of forms of hepcidin.That is to say,it detect total hepcidin.
If further questions,pls let us know.

Target Background

Function
(From Uniprot)
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
Gene References into Functions
  1. Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-kappaB Activation. PMID: 29147463
  2. In experimental autoimmune encephalomyelitis, we recently found that chronic iron overload influences the course of disease. In females, iron overload accelerated the onset of disease, while in males it accelerated the progression of disease and increased mortality rate. We hypothesize that those differences arise on molecular level in different expression of stress response proteins hepcidin and metallothioneins. PMID: 28915963
  3. Anti-hemojuvelin antibody corrects anemia caused by inappropriately high hepcidin levels PMID: 26944476
  4. when a high erythropoietic stimulus occurs, hepcidin synthesis is mainly regulated by transferrin saturation; however, when the erythropoiesis rate reaches a specific threshold, extramedullary hematopoiesis is triggered, and the control of hepcidin synthesis is switched to matriptase-2, thus inhibiting hepcidin synthesis. PMID: 27282570
  5. The data of in vitro and in vivo research evidenced on involvement of Hepc in formation of breast cancer cells malignant phenotype and their resistance to doxorubicin. PMID: 27356575
  6. downregulation of ferroportin-1 and ceruloplasmin caused by hepcidin enhanced iron-dependent oxidative damage and may be the potential mechanism of subarachnoid hemorrhage . PMID: 27250827
  7. liver iron overload was an important stimuli for hepcidin synthesis, stronger than the inhibitory effect of high rHuEPO doses; moreover, the findings raised the hypothesis that when high inflammation (triggering hepcidin expression) was associated with increased iron stores in hemodialysis patients, hepcidin expression was also upregulated via BMP6, enhancing hepcidin synthesis, leading, therefore, to worsening of anemia PMID: 26990350
  8. Rats were treated with different dose levels of a monoclonal antibody that downregulates Hepcidin; results of this morphometric analysis in the liver showed that iron accumulation is not homogenous between liver lobes and the left lateral lobe was the most responsive lobe in the rat. PMID: 26839325
  9. Hepcidin may be considered as a potential marker of impaired renal function. PMID: 26907911
  10. a significant decrease of hepcidin expression is observed during late-pregnancy and early-lactation stages, suggesting the important regulatory role that hepcidin plays in iron metabolism during pregnancy and lactation PMID: 26788496
  11. Study investigated the expression of hepcidin mRNA and protein in normal rat brain and to interpret the findings in the light of the properties of hepcidin as a peptide hormone with an essential role in systemic iron homeostasis PMID: 25896789
  12. liver congestion induces hepcidin production, which may result in anemia and functional iron deficiency in some patients with heart failure. PMID: 25742771
  13. Vitamin A deficiency reduced serum iron and transferrin saturation levels, increased spleen iron concentrations, reduced hepatic Hamp and kidney erythropoietin messenger RNA levels and up-regulated hepatic and spleen heme oxygenase-1 gene expression. PMID: 24998947
  14. Data indicate that a moderate decrease in hepatic hepcidin mRNA content was observed in male rats. PMID: 24962641
  15. inhibition of hepcidin may reduce many pathological changes seen in stress-induced depressive disorders. PMID: 25576700
  16. This leads to a serum iron increase, which seems to stimulate hepcidin expression despite no evidence of inflammation, thus suggesting iron as the key modulator of hepcidin synthesis PMID: 25580431
  17. Colitis increased local hepcidin-25 expression, which was associated with the IL-6/Stat-3 signaling pathway PMID: 24764672
  18. High hepcidin serum expression levels correlated with an impaired hematologic response to an erythropoiesis-stimulating agent in rats with anemia of chronic disease. PMID: 24895335
  19. Hepcidin is increased in the hypertrophied heart of Dahl salt-sensitive rats. PMID: 24424338
  20. Data indicate that Hepcidin expression is increased in response to serum treatment in liver cells. PMID: 25151311
  21. Data suggest that hepcidin was a relevant indicator for renal I/R injury diagnosis. PMID: 23439664
  22. hepcidin inhibits expression of iron release as well as iron uptake proteins in macrophages PMID: 22560353
  23. Angelica sinensis polysaccharide significantly reduced hepcidin expression by inhibiting the expression of (SMAD4) in liver and stimulating the secretion of erythropoietin. PMID: 22985399
  24. result strongly suggests that plasma Il-6 is involved in exercise-induced increase of hepcidin gene expression PMID: 22326661
  25. We found that hepcidin mRNA levels were significantly reduced in the regenerating liver after the acute-phase response was completed. PMID: 22364558
  26. Pathways for the regulation of hepcidin expression in anemia of chronic disease and iron deficiency anemia PMID: 21859731
  27. Hepcidin controls iron uptake and release by regulating expression of iron transport proteins, implying the existence of a novel hepcidin-receptor on the membrane of astrocytes. PMID: 21438013
  28. hepcidin is an important contributor to iron overload in cerebral ischemia PMID: 21957487
  29. Downregulation of hepcidin (Hamp) gene, a key regulator of Fpn1 was accompanied by decreased levels of CCAAT/enhancer binding proteins alpha and beta, especially at the Hamp promoter. PMID: 21785164
  30. Although liver Fe concentration was significantly higher in rats fed an Mg-deficient diet for 4 weeks than in rats fed a control diet, Hepcidin expression in the liver was comparable between the dietary groups. PMID: 21736832
  31. hepcidin inhibits the expression of iron-absorption proteins and led to exercise-associated anemia. PMID: 21411831
  32. Matriptase-2- and proprotein convertase-cleaved forms of hemojuvelin have different roles in the down-regulation of hepcidin expression PMID: 20937842
  33. investigation of up-regulation of hepcidin in myocardium following myocardial infarction; comparison of ischemic tissue vs. remote myocardial tissue PMID: 20553779
  34. With the increases of iron levels in diet, the levels of serum iron, serum ferretin, and transferrin saturation were gradually increased, and the hepcidin mRNA expression level in liver significantly increased. PMID: 18839536
  35. regulation of hepatic transcription by C/EBPalpha PMID: 12183449
  36. hepcidin mediates lipopolysaccharide-induced downregulation of intestinal ferroportin 1 expression and the hepcidin signaling pathway involves a pyrrolidinedithiocarbamate-sensitive step PMID: 14592944
  37. In liver ischemia-reperfusion model of acute inflammation, mechanism(s) other than interleukin-6 signal transduction via signal transducers and activators of transcription-3 may be responsible for hepcidin induction. PMID: 15973703
  38. HAMP expression differs in cultured as compared with freshly isolated hepatocytes, and decreases in iron-loaded hepatocytes in serum free-media, suggesting that additional serum factors influence HAMP expression. PMID: 16221503
  39. Hepcidin may have an important role in cardiac diseases. PMID: 17363462
  40. hepcidin may play a major, causative role in the change of FPN1 synthesis PMID: 18189270
  41. Lipopolysaccharides induced a significant increase in the expression of hepcidin mRNA and protein in the cortex and substantia nigra. PMID: 18450970
  42. IL-6-hepcidin axis is up-regulated by psychological stress in rats. PMID: 18541141
  43. overproduction of HIF-1alpha and the activation of caspase-3 seem to be associated with iron deficiency and with inflammatory markers. Hepcidin seems to plays a key role in this mechanism. PMID: 18808386
  44. Regulation of liver hepcidin expression by alcohol in vivo does not involve Kupffer cell activation or TNF-alpha signaling. PMID: 19008338
  45. Phagocytosis upregulates hepcidin-gene expression and downregulates Hjv- and Fpn-1-gene expression within the liver. PMID: 19721414

Show More

Hide All

Subcellular Location Secreted.
Protein Families Hepcidin family
Database Links

KEGG: rno:84604

STRING: 10116.ENSRNOP00000028545

UniGene: Rn.7865

Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
Join Us with

Subscribe newsletter

Leave a message

© 2007-2022 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1