Rat Parathyroid hormone,PTH ELISA Kit

Code CSB-E07866r
Size 96T,5×96T,10×96T
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Product Details

Target Name
parathyroid hormone
Alternative Names
Pth ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
Abbreviation
PTH
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:5Average %97
Range %91-103
1:10Average %99
Range %95-107
1:20Average %95
Range %90-99
1:40Average %87
Range %81-92
Recovery
The recovery of rat PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-97
EDTA plasma (n=4)106102-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.613 2.511 2.562 2.408
2002.054 1.953 2.004 1.850
1001.276 1.287 1.282 1.128
500.727 0.738 0.733 0.579
250.457 0.468 0.463 0.309
12.50.361 0.350 0.356 0.202
6.250.275 0.269 0.272 0.118
00.154 0.153 0.154  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat PTH ELISA Kit was designed for the quantitative measurement of Rat PTH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

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 Q&A
Q:

Does the rat PTH ELISA Kit has a removable strip?

A:

Yes, it does. CUSABIO ELISA kits are all with removable strips.

Target Background

Function
(From Uniprot)
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Gene References into Functions
  1. PTHrP and PTH mediate wasting through a common mechanism involving PTHR. PMID: 26669699
  2. our data demonstrate that PTH activation of nuclear receptor-associated protein 1 is essential for the effect of PTH to increase FGF23 levels. PMID: 24940803
  3. data support a link between PTH regulating HDAC4 phosphorylation by PKA, trafficking, partial degradation, and the control of MMP-13 transcription through association with Runx2 PMID: 24904057
  4. Mg reduces PTH secretion mainly when a moderate low calcium concentration is present; Mg also modulates parathyroid glands function through upregulation of the key cellular receptors CaR, VDR and FGF23/Klotho system. PMID: 24103811
  5. The persistence of the phosphaturic response to PTH occurs through posttranscriptional mechanisms. PMID: 23344572
  6. The secretory response of parathyroid hormone to acute hypocalcemia in vivo is independent of parathyroid glandular sodium/potassium-ATPase activity. PMID: 21209610
  7. Data suggest that a high concentration of PTH(1-34)-induced cytotoxicity and apoptosis in PC12 cells was associated with upregulation of ERK and p38 through a mitochondria-mediated apoptotic pathway. PMID: 20971569
  8. Study indicates an influence of sex on PTH molecular form secretion or metabolism. PMID: 20627105
  9. Modulation of integrin expression in osteoblastic cells by PTH is likely to be indirect, and phenotypic expression of integrin subtypes is part of the cascade of events that lead to PTH (1-34) mediated osteoblast differentiation. PMID: 11746505
  10. New signaling pathway for parathyroid hormone and cyclic AMP action on extracellular-regulated kinase and cell proliferation in bone cells PMID: 11956184
  11. alterations in PI3K activity in response to parathyroid hormone with aging PMID: 12581861
  12. PTH or PTHrP can exert anabolic effects in the skeleton. PMID: 12631114
  13. that periosteal and endosteal osteoblastic cell populations exhibit metabolic differences in their extracellular matrix responses to PTH PMID: 14514685
  14. the parathyroid hormone-Smad3 axis may have a role in the bone anabolic action of PTH PMID: 14517210
  15. PTH and mechanical stimulation on trabecular bone formation rate suggest a potential benefit for combined PTH administration and exercise in the treatment of osteoporosis. PMID: 14672346
  16. Rho and Rho kinase are an important component of PTH signaling in osteoblastic cells PMID: 15476589
  17. Combinations of radioprotectors may achieve their beneficial additive effects in the growth plate by decreasing the usual early drop in parathyroid hormone-related protein and parathyroid hormone receptor 1 after irradiation PMID: 16494524
  18. Calcium, phosphate & uremia determine parathyroid hormone mRNA stability through binding of protective factors adenosine-uridine rich binding factor 1 & Up-stream of N-ras & recruitment of degradation complex that cleaves parathyroid hormone mRNA[review] PMID: 17565271
  19. FGF23 suppressed both parathyroid hormone (PTH) secretion and PTH gene expression PMID: 17992255
  20. IL-18 is regulated by parathyroid hormone and is required for its bone anabolic actions PMID: 18165223
  21. These data indicate a novel role for KSRP in parathyroid hormone gene expression. PMID: 18583400
  22. Phosphate overload and normal parathyroid hormone infusion induced phenotypic changes in vascular smooth muscle cells, as evidenced by upregulated aortic expression of Runx2. PMID: 19075196
  23. Regulation of PTH mRNA stability by the calcimimetic R568 and the phosphorus binder lanthanum carbonate in chronic kidney disease. PMID: 19129257
  24. without altering the gross chromatin structure, PTH stimulates acetylation of histones H3 and H4 via recruitment of p300 to Runx2 bound to the MMP-13 promoter, resulting in gene activation PMID: 19423655
  25. estrogens regulate PTH indirectly, possibly through FGF23 PMID: 19628670
  26. Parathyroid hormone (PTH) suppresses rat PTH/PTH-related protein receptor gene promoter PMID: 11554727

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Subcellular Location
Secreted.
Protein Families
Parathyroid hormone family
Tissue Specificity
Hypothalamus and parathyroid gland.
Database Links
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