Rat Tau Proteins ELISA kit

Code CSB-E13729r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rat MAPT ELISA Kit was designed for the quantitative measurement of Rat MAPT protein in serum, plasma, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Target Name microtubule-associated protein tau
Alternative Names
Mapt ELISA Kit; Mtapt ELISA Kit; Tau ELISA Kit; Microtubule-associated protein tau ELISA Kit; Neurofibrillary tangle protein ELISA Kit; Paired helical filament-tau ELISA Kit; PHF-tau ELISA Kit
Abbreviation MAPT
Uniprot No. P19332
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cerebrospinal fluid (CSF)
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of rat tau proteins in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:5Average %98
Range %95-101
1:10Average %90
Range %88-92
1:20Average %85
Range %81-89
1:40Average %98
Range %95-101
The recovery of rat tau proteins spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10199-105
EDTA plasma (n=4)8784-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
40002.694 2.678 2.686 2.523
20002.104 2.156 2.130 1.967
10001.330 1.356 1.343 1.180
5000.825 0.836 0.831 0.668
2500.552 0.568 0.560 0.397
1250.359 0.368 0.364 0.201
62.50.276 0.298 0.287 0.124
00.167 0.159 0.163
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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I want to know if CSB-E13729r detects Tau regardless of phosphorylation state?

Thanks for your inquiry.
CSB-E13729r detect the total Tau, including the phosphorylated form.Pls let me know if you have any further questions. Thank you.

Target Background

(From Uniprot)
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Subcellular Location Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted.
Tissue Specificity Expressed in neurons. The larger forms (isoform tau-A and isoform tau-B) are preferentially expressed in the peripheral nervous system while the other are expressed in the central nervous system. Low amounts of the larger forms are also found in limited a
Database Links

STRING: 10116.ENSRNOP00000006856

UniGene: Rn.2455

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