Rat Tau Proteins ELISA kit

Code CSB-E13729r
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P19332
Lead Time 3-5 working days
Abbreviation MAPT
Target Name microtubule-associated protein tau
Alias DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU, G protein beta1/gamma2 subunit-interacting factor 1|microtubule-associated protein tau, isoform 4
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cerebrospinal fluid (CSF)
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer s disease, Pick s disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat tau proteins in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:5Average %98
Range %95-101
1:10Average %90
Range %88-92
1:20Average %85
Range %81-89
1:40Average %98
Range %95-101
Recovery
The recovery of rat tau proteins spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10199-105
EDTA plasma (n=4)8784-90
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
40002.694 2.678 2.686 2.523
20002.104 2.156 2.130 1.967
10001.330 1.356 1.343 1.180
5000.825 0.836 0.831 0.668
2500.552 0.568 0.560 0.397
1250.359 0.368 0.364 0.201
62.50.276 0.298 0.287 0.124
00.167 0.159 0.163
Still Have Questions? Leave a Message or Start an on-line Chat
Function Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Subcellular Location Cytoplasm, cytosol, Cell membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, cytoskeleton, Cell projection, axon
Tissue Specificity Expressed in neurons. The larger forms (isoform tau-A and isoform tau-B) are preferentially expressed in the peripheral nervous system while the other are expressed in the central nervous system. Low amounts of the larger forms are also found in limited a
Database Links

STRING: 10116.ENSRNOP00000006856

UniGene: Rn.2455

Pathway MAPK signaling pathway

Related Products

Mapt Antibodies

Mapt Antibodies for Rattus norvegicus (Rat)

Mapt Proteins

Mapt Proteins for Rattus norvegicus (Rat)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.