Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Rat Mapt at 2 μg/ml can bind anti-MAPT recombinant antibody (CSB-RA013481A1HU), the EC50 is 136.2-238.9 ng/mL.
Alternative Names
(Neurofibrillary tangle protein)(Paired helical filament-tau)(PHF-tau)
Molecular Characterization
Species
Rattus norvegicus (Rat)
Expression Region
2-752aa
Target Protein Sequence
AEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Rat Mapt recombinant protein was produced in mammalian cell, where the gene sequence encoding rat Mapt (2-752aa) was expressed with the N-terminal 10xHis tag. The purity of this Mapt protein was greater than 95% by SDS-PAGE. The activity has been validated.
Tau protein is a highly soluble microtubule-associated protein (MAP) encoded by the Mapt gene. Tau protein is a basic protein. As a member of the MAPs family, tau protein mainly acts on the distal end of axons to maintain the stability and flexibility of microtubules. Tau protein interacts with Tubulin to stabilize microtubules, while driving Tubulin assembly within microtubules. Tau proteins control microtubule stability through isomerization and phosphorylation.
Tau protein is involved in regulating axonal transport and nuclear function to protect DNA integrity; interacts with actin cytoskeleton to promote actin filament formation; and regulates NMDA receptor signaling pathway by interacting with Fyn.
Phosphorylation of tau is regulated by a number of kinases, including PKN, serine/threonine kinases, whose activation causes microtubule tissue disruption. The accumulation of hyperphosphorylated tau in neurons will cause neurofibrillary degeneration, which is associated with various neurodegenerative diseases such as AD and PD.
