Rat Tissue factor,TF ELISA Kit

Code CSB-E07914r
Size 96T,5×96T,10×96T
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Product Details

Target Name
coagulation factor III (thromboplastin, tissue factor)
Alternative Names
F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit
Abbreviation
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, urine, cell culture supernates, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Blood Coagulation
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %91-106
1:2Average %90
Range %85-96
1:4Average %93
Range %89-100
1:8Average %99
Range %93-108
Recovery
The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-94
EDTA plasma (n=4)10395-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.297 2.231 2.264 2.081
10001.573 1.471 1.522 1.339
5000.999 0.924 0.962 0.779
2500.618 0.593 0.606 0.423
1250.405 0.425 0.415 0.232
62.50.293 0.310 0.302 0.119
31.250.249 0.264 0.257 0.074
00.177 0.188 0.183  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat tissue factor (TF) ELISA Kit was designed for the quantitative measurement of Rat tissue factor (TF) protein in serum, urine, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL .

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Target Background

Function
(From Uniprot)
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Tissue factor family
Database Links
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