Rat Tissue factor,TF ELISA Kit

Instructions
Code CSB-E07914r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name coagulation factor III (thromboplastin, tissue factor)
Alternative Names F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit
Abbreviation F3
Uniprot No. P42533
Species Rattus norvegicus (Rat)
Sample Types serum, urine, cell culture supernates, tissue homogenates, cell lysates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Blood Coagulation
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %102
Range %91-106
1:2Average %90
Range %85-96
1:4Average %93
Range %89-100
1:8Average %99
Range %93-108
Recovery
The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8884-94
EDTA plasma (n=4)10395-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.297 2.231 2.264 2.081
10001.573 1.471 1.522 1.339
5000.999 0.924 0.962 0.779
2500.618 0.593 0.606 0.423
1250.405 0.425 0.415 0.232
62.50.293 0.310 0.302 0.119
31.250.249 0.264 0.257 0.074
00.177 0.188 0.183  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Tissue factor family
Database Links

STRING: 10116.ENSRNOP00000015836

UniGene: Rn.9980

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