Rat Tissue factor,TF ELISA Kit

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Citations

Customer Reviews and Q&A

Target Data

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Rat Tissue factor,TF ELISA Kit

Name: Rat Tissue factor,TF ELISA Kit
Brand: CUSABIO
SKU: CSB-E07914r

^Instructions

Code	CSB-E07914r
Size	96T,5×96T,10×96T
Trial Size
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Product Details
Citations
Customer Reviews and Q&A
Target Data

Target Name

coagulation factor III (thromboplastin, tissue factor)

Alternative Names

F3 ELISA Kit; Cf3 ELISA Kit; Tissue factor ELISA Kit; TF ELISA Kit; Coagulation factor III ELISA Kit; CD antigen CD142 ELISA Kit

Abbreviation

Uniprot No.

P42533

Species

Rattus norvegicus (Rat)

Sample Types

serum, urine, cell culture supernates, tissue homogenates, cell lysates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.8 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Blood Coagulation

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	91-106
1:2	Average %	90
	Range %	85-96
1:4	Average %	93
	Range %	89-100
1:8	Average %	99
	Range %	93-108

Recovery

The recovery of rat TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-94
EDTA plasma (n=4)	103	95-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.297	2.231	2.264	2.081
1000	1.573	1.471	1.522	1.339
500	0.999	0.924	0.962	0.779
250	0.618	0.593	0.606	0.423
125	0.405	0.425	0.415	0.232
62.5	0.293	0.310	0.302	0.119
31.25	0.249	0.264	0.257	0.074
0	0.177	0.188	0.183

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

BAY11-7082 inhibits the expression of tissue factor and plasminogen activator inhibitor-1 in type-II alveolar epithelial cells following TNF-α stimulation via the NF-κB pathway Y Cheng,Experimental and Therapeutic Medicine,2021
Hemostatic effect of acylated ghrelin in control and sleeve gastrectomy-induced rats: mechanisms of action Morsy MD.et al,ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY,2018
Particulate matter phagocytosis induces tissue factor in differentiating macrophages Milano M et al,J Appl Toxicol ,2015

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Function	Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF
Subcellular Location	Membrane, Single-pass type I membrane protein
Protein Families	Tissue factor family
Database Links	STRING: 10116.ENSRNOP00000015836 UniGene: Rn.9980

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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