Rabbit Endothelin 1,ET-1 ELISA Kit

Instructions
Code CSB-E06951Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name endothelin 1
Alternative Names EDN1Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
Abbreviation EDN1
Uniprot No. P29560
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-92
1:2Average %100
Range %91-105
1:4Average %96
Range %92-104
1:8Average %94
Range %86-98
Recovery
The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-99
EDTA plasma (n=4)9890-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.630 2.456 2.543 2.458
2002.111 2.076 2.094 2.009
1001.459 1.443 1.451 1.366
500.816 0.769 0.793 0.708
250.415 0.389 0.402 0.317
12.50.218 0.203 0.211 0.126
6.250.147 0.149 0.148 0.063
00.088 0.082 0.085  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
Gene References into Functions
  1. Study shows that cerebrovascular muscle cells can express endothelin-1 (ET-1). The production of ET-1 in cerebrovascular muscle cells stimulated by oxyhemoglobin occurs partially through the NF-[kappa]B signal pathway. PMID: 27391329
  2. Plasma ET-1 levels of rabbits increased significantly in fluorinated groups compared with those in the control group. PMID: 27941335
  3. Dynamic monitoring and comparison of plasma levels of ET, CGRP, NO, and MDA as well as SOD activity revealed that appropriate intervention of these factors may reduce reperfusion injury PMID: 26125755
  4. In a saline lavage-induced lung injury model, both circulatory and pulmonary ET-1 levels increased. PMID: 24735960
  5. Data suggest elevated levels of endothelin-1, as exhibited in cardiovascular diseases, facilitate development of ventricular arrhythmia by steepening action potential duration restitution and by increasing beat-to-beat variability of repolarization. PMID: 24084217
  6. High levels of ET-1 are closely associated with BBB disruption. ET-1 may play an important role in the pathogenesis of secondary brain injury after ICH. PMID: 24034085
  7. After subarachnoid hemorrhage, the contractile response to ET-1 was enhanced, and the ET(A) receptor expression was upregulated in the basilar artery. PMID: 21952110
  8. After acute pulmonary thromboembolism, thrombolytic and anti-inflammatory treatment could decrease acute lung injury induced by ET-1 and NF-kappaB activation. [endothelin-1; NFKB] PMID: 16207427
  9. The increase in myocardial distensibility induced by endothelin-1 is absent in heart failure and is dependent on nitric oxide and prostaglandin release. PMID: 17552879
  10. Endothelin-1 enhances nuclear Ca2+ transients in atrial myocytes through Ins(1,4,5)P3-dependent Ca2+ release from perinuclear Ca2+ stores PMID: 18089647
  11. The enhancement of gap junction intercellular communication is activated by endothelin-1 via modulating the expression of connexin43, and plays an important role in the pathogenesis of cerebral vasospasm PMID: 18691452

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Subcellular Location Secreted.
Protein Families Endothelin/sarafotoxin family
Database Links

KEGG: ocu:100009270

STRING: 9986.ENSOCUP00000011526

UniGene: Ocu.5758

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