Rabbit Endothelin 1,ET-1 ELISA Kit

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Rabbit Endothelin 1,ET-1 ELISA Kit

Citations (2) Protocol

Code	CSB-E06951Rb
Size	96T,5×96T,10×96T How to order?
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Citations (2)
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Description

This Rabbit EDN1 ELISA Kit was designed for the quantitative measurement of Rabbit EDN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Name

endothelin 1

Alternative Names

EDN1Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit

Abbreviation

EDN1

Uniprot No.

P29560

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, tissue homogenates

Detection Range

6.25 pg/mL-400 pg/mL

Sensitivity

1.56 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	100
	Range %	91-105
1:4	Average %	96
	Range %	92-104
1:8	Average %	94
	Range %	86-98

Recovery

The recovery of rabbit ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	98	90-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.630	2.456	2.543	2.458
200	2.111	2.076	2.094	2.009
100	1.459	1.443	1.451	1.366
50	0.816	0.769	0.793	0.708
25	0.415	0.389	0.402	0.317
12.5	0.218	0.203	0.211	0.126
6.25	0.147	0.149	0.148	0.063
0	0.088	0.082	0.085

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

CT perfusion imaging of cerebral microcirculatory changes following subarachnoid hemorrhage in rabbits: Specific role of endothelin-1 receptor antagonist QuanyangWu.et al,Brain Research Bulletin,2018
Blast Wave Exposure to the Extremities Causes Endothelial Activation and Damage. Spear AM.et al,Shock.,2015

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Function (From Uniprot)	Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
Gene References into Functions	Study shows that cerebrovascular muscle cells can express endothelin-1 (ET-1). The production of ET-1 in cerebrovascular muscle cells stimulated by oxyhemoglobin occurs partially through the NF-[kappa]B signal pathway. PMID: 27391329 Plasma ET-1 levels of rabbits increased significantly in fluorinated groups compared with those in the control group. PMID: 27941335 Dynamic monitoring and comparison of plasma levels of ET, CGRP, NO, and MDA as well as SOD activity revealed that appropriate intervention of these factors may reduce reperfusion injury PMID: 26125755 In a saline lavage-induced lung injury model, both circulatory and pulmonary ET-1 levels increased. PMID: 24735960 Data suggest elevated levels of endothelin-1, as exhibited in cardiovascular diseases, facilitate development of ventricular arrhythmia by steepening action potential duration restitution and by increasing beat-to-beat variability of repolarization. PMID: 24084217 High levels of ET-1 are closely associated with BBB disruption. ET-1 may play an important role in the pathogenesis of secondary brain injury after ICH. PMID: 24034085 After subarachnoid hemorrhage, the contractile response to ET-1 was enhanced, and the ET(A) receptor expression was upregulated in the basilar artery. PMID: 21952110 After acute pulmonary thromboembolism, thrombolytic and anti-inflammatory treatment could decrease acute lung injury induced by ET-1 and NF-kappaB activation. [endothelin-1; NFKB] PMID: 16207427 The increase in myocardial distensibility induced by endothelin-1 is absent in heart failure and is dependent on nitric oxide and prostaglandin release. PMID: 17552879 Endothelin-1 enhances nuclear Ca2+ transients in atrial myocytes through Ins(1,4,5)P3-dependent Ca2+ release from perinuclear Ca2+ stores PMID: 18089647 The enhancement of gap junction intercellular communication is activated by endothelin-1 via modulating the expression of connexin43, and plays an important role in the pathogenesis of cerebral vasospasm PMID: 18691452 Show More Hide All
Subcellular Location	Secreted.
Protein Families	Endothelin/sarafotoxin family
Database Links	KEGG: ocu:100009270 STRING: 9986.ENSOCUP00000011526 UniGene: Ocu.5758

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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