Rabbit Immunoglobulin A,IgA ELISA Kit

Code CSB-E06946Rb
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgA
Target Name Immunoglobulin A,IgA
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 12 ng/mL-3000 ng/mL
Sensitivity 3 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1000Average %89
Range %84-97
1:2000Average %91
Range %89-96
1:4000Average %93
Range %87-104
1:8000Average %92
Range %86-98
Recovery
The recovery of rabbit IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9788-103
EDTA plasma (n=4)9585-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2Average
30000.221 0.218 0.220
7500.351 0.354 0.353
187.50.629 0.625 0.627
471.464 1.502 1.483
122.259 2.235 2.247
03.218 3.155 3.187
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