Rabbit tissue inhibitors of metalloproteinase 1,TIMP-1 ELISA Kit

Instructions
Code CSB-E06935Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name TIMP metallopeptidase inhibitor 1
Alternative Names TIMP1 ELISA Kit; Metalloproteinase inhibitor 1 ELISA Kit; Tissue inhibitor of metalloproteinases 1 ELISA Kit; TIMP-1 ELISA Kit
Abbreviation TIMP1
Uniprot No. P20614
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, cell culture supernates , tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TIMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 85-97  
1:200 Average % 102  
Range % 98-108  
1:400 Average % 98  
Range % 94-102  
1:800 Average % 88  
Range % 84-93  
Recovery
The recovery of rabbit TIMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
EDTA plasma (n=4) 92 88-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
1000 0.086 0.084 0.085    
500 0.145 0.141 0.143    
250 0.245 0.238 0.242    
125 0.392 0.413 0.403    
62.5 0.725 0.727 0.726    
31.2 0.963 0.944 0.954    
15.6 1.154 1.179 1.167    
0 1.734 1.662 1.698    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling (By similarity).
Gene References into Functions
  1. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
Subcellular Location Secreted
Protein Families Protease inhibitor I35 (TIMP) family
Database Links

KEGG: ocu:100009047

STRING: 9986.ENSOCUP00000009228

UniGene: Ocu.178

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