Recombinant Human Fibroblast growth factor 1(FGF1) (Active)

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Code CSB-AP003761HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined in a cell proliferation assay using BALB/c 3T3 cells is less than 5 ng/ml.
Target Names FGF1
Uniprot No. P05230
Research Area Signal Transduction
Alternative Names Acidic fibroblast growth factor; aFGF; Beta endothelial cell growth factor; Beta-endothelial cell growth factor; ECGF; ECGF beta; ECGF-beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fgf1; FGF1_HUMAN; FGFA; Fibroblast Growth Factor 1 Acidic; Fibroblast growth factor 1; GLIO703; HBGF 1; HBGF-1; HBGF1; Heparin binding growth factor 1; Heparin binding growth factor 1 precursor; Heparin-binding growth factor 1
Species Homo sapiens (Human)
Source E.coli
Expression Region 16-155aa
Complete Sequence FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Mol. Weight 15.9 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm Filtered 20 mM Tris, 400 mM NaCl, 1 mM DTT, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV
Gene References into Functions
  1. Gas5 regulated proliferation and apoptosis in growth plate by controlling FGF1 expression via miR-21 regulation. PMID: 29490650
  2. Modification of the heparin-binding region of FGF1 significantly improves the cardioprotective efficacy, even in the presence of heparin, identifying a novel FGF ligand available for therapeutic use in ischemic heart disease. PMID: 29016740
  3. FGF1 protects neuroblastoma cells from p53-dependent apoptosis through an intracrine pathway regulated by FGF1 phosphorylation. PMID: 29048426
  4. Specific heparin derivatives molecular recognition patterns by FGF1 have been reported. PMID: 27858202
  5. Multiple stepwise linear regression analysis found serum level of FGF1 was dependent on anti-diabetic drugs, hemoglobin A1C, body mass index and sex. Serum level of FGF1 is associated with the decreased risk of obesity in human. PMID: 28303556
  6. Our study suggests that the genetic variants of FGF1 rs34011, more so than FGF2 rs2922979, may play a role in PE pathogenesis in Tunisian women. PMID: 27324104
  7. FGF1 and 2 strongly prevent the osteogenic commitment and differentiation of hBMSCs. PMID: 27305863
  8. Transfected FGF1 promotes angiogenic proliferation. PMID: 28281780
  9. These observations suggest a crucial role for cancer-associated fibroblasts and fibroblast growth factor-1/fibroblast growth factor receptor 4 signaling in the progression of ovarian cancer. the expression level of Snail1 and MMP3 was reduced, while the expression level of E-cadherin increased PMID: 28718374
  10. Enzyme-linked immunosorbent assay was used to detect the levels of chemokine (C-X-C motif) ligand 12, chemokine (C-X-C motif) ligand 7, hepatocyte growth factor, and fibroblast growth factor 1 in the supernatants of the laryngeal squamous cell carcinoma and control cells. PMID: 28475003
  11. fibroblast growth factor 1 (FGF1) to be synergistically induced by heat shock and wounding. PMID: 27638903
  12. The results indicate that suramin blocks the interaction between hFGF1 and FGFR2 D2. PMID: 27387234
  13. activation of AurA kinase through FGF1/FGFR signaling axis sustains the stem cell characteristics of glioblastoma cells. PMID: 27138904
  14. Study showed that both aFGF and bFGF were highly expressed in cervical cancer tissues. In tumors of higher clinical stages, the expression of these factors was further enhanced, suggesting that they play a role in facilitating cervical cancer cell proliferation. PMID: 27966750
  15. FGF1 may play a role in the pathogenesis of T2 diabetes mellitus. PMID: 26806193
  16. anti-importin alpha1 antibody treatment suppressed the importin alpha1-FGF1 complex formation and ERK1/2 activation, resulting in decreased cell growth. This study provides novel evidence that functional importin alpha1 is located at the cell surface, where it accelerates the proliferation of cancer cells. PMID: 26887791
  17. The mutational introduction of a novel Cys residue (Ala66Cys) that forms a stabilizing disulfide bond (i.e., cystine) with one of the extant Cys residues (Cys83) effectively eliminates one Cys while increasing overall stability. PMID: 27019961
  18. The role of intracellular FGF1 is to protect the cell against stress conditions by providing an additional signal for cell survival, independently of receptor-activated signaling cascades. PMID: 26840910
  19. Data suggest folding of FGF1 is critical for its nonclassical secretion via permeability of lipid bilayer; mutation of proline135 in C-terminus of FGF1 leads to partial unfolding/decrease in FGF1's ability to permeabilize phosphatidylserine bilayers. PMID: 26836284
  20. The analysis identified a signaling axis between FGF signaling and the transcription factor Sox1, which is preferentially expressed in stem- and mesenchymal-like breast cancers. PMID: 26365194
  21. FGF-1 synthesis and secretion by synovial fibroblasts were significantly increased in osteoarthritis. PMID: 26400350
  22. expression of human FGF1 solely in beta-cells in fgf1(-/-) animals prevented overnutrition induced compensatory beta-cell differentiation. PMID: 26420862
  23. Results show that stress functionally associates FGF1 with AHNAK2 and both proteins with the cytoskeleton and their co-localization in the vicinity of the cell membrane. AHNAK2 seems to be an important element of the FGF1 export pathway. PMID: 25560297
  24. These findings suggest that the presence of FGF1 may serve as a prognostic indicator and a potential therapeutic target for non-small cell lung cancer patients, especially for lung squamous cell carcinoma. PMID: 26391572
  25. FGF1 expression is increased in lung from idiopathic pulmonary fibrosis patients. PMID: 26138239
  26. FGF1-FGFR1 axis promotes tongue squamous cell carcinoma metastasis through the epithelial-mesenchymal transition pathway PMID: 26362179
  27. p120RasGAP shields Akt from deactivating phosphatases in FGF1 signaling, but loses this ability once cleaved by caspase-3. PMID: 26109071
  28. Study presents a transcript profiling of remyelinated multiple sclerosis lesions and identified FGF1 as a promoter of remyelination PMID: 25589163
  29. These results suggested that human FGF1B promoter was active in ependymal cells, neurons, and a portion of dopaminergic neurons PMID: 25104610
  30. This review is focused on the role of HDGF in tumorigenesis and metastasis, and provides insight for application in clinical cancer therapy as well as its clinical implications as a prognostic marker in cancer progression. PMID: 25236340
  31. the sulfate in position 6 of d-glucosamine was essential for the mitogenic activity but not for the interaction with FGF-1 PMID: 25015527
  32. While results are awaited from these clinical investigations in squamous NSCLC and other disease settings, additional research is needed to elucidate the role of FGF/FGFR signaling in the biology of NSCLC of different histologies PMID: 24711160
  33. FGF1 gene polymorphism is associated with a lower risk of developing cleft palate or cleft lip in Iranian patients. clet palatot cleft lip PMID: 24613087
  34. precursor of the hormone Irisin (FNCD5) were abundantly expressed in all three fat depots, along with fibroblast growth factors (FGF) FGF1, FGF7 and FGF10, whereas, FGF19 and FGF21 were undetectable. PMID: 23949615
  35. Nucleolin-FGF1 interaction is critical for the intranuclear phosphorylation of FGF1 by PKCdelta and thereby the regulation of nuclear export of FGF1. PMID: 24595027
  36. the genotype distribution of rs34011 within fibroblast growth factor 1 was significantly different between Alzheimer's disease and control group PMID: 24464990
  37. discovery of unexpected, neomorphic insulin-sensitizing action for exogenous non-mitogenic human FGF1 with therapeutic potential for the treatment of insulin resistance and type 2 diabetes PMID: 25043058
  38. By introducing two stabilizing mutations in the C-terminal part of the protein, obtained are variants highly resistant proteolytically with prolonged half-life and enhanced mitogenic activity. PMID: 24304385
  39. Data suggest that the enhanced cell growth was likely due to the electrical stimulation up-regulated secretion of FGF-1 and FGF-2. PMID: 23990967
  40. Both FGF1 and ERBB2 significantly influenced overall survival in breast cancer patients, especially among women with low levels of Native American ancestry. PMID: 23912956
  41. We demonstrate that mesenchymal stromal cells increase FGF-1 secretion on co-culture with Human primary chondrocytes, which, in turn, is responsible for increased Human primary chondrocytes proliferation in pellet co-cultures. PMID: 23557133
  42. FGF1 polymorphism is associated with breast cancer. PMID: 23143756
  43. R50E suppresses angiogenesis induced by FGF1 or FGF2, and thereby indirectly suppresses tumorigenesis, in addition to its possible direct effect on tumor cell proliferation in vivo PMID: 23469107
  44. studied the functional consequences of HSulf-2 activity on fibroblast growth factor (FGF)-induced mitogenesis and found that the enzyme could differentially regulate FGF1 and FGF2 activities PMID: 23457216
  45. The expression of FGF-1 in the in vitro fertilization (IVF) implantation failure group is less than in the fertile group, which suggests that growth factors such as FGF-1 are important maternal factors effecting implantation PMID: 23426545
  46. forms of FGF-1 that enhance thermostability or eliminate buried reactive thiols demonstrate a shorter distribution half-life, a longer elimination half-life, and a longer mean residence time (MRT) in comparison to wild-type FGF-1 PMID: 23133616
  47. The folding transition state of FGF-1 is shown to be highly polarized, with the majority of turns adopting either native-like or denatured-like structure in the folding transition state. PMID: 23047594
  48. LRRC59 facilitates transport of cytosolic FGF1 through nuclear pores by interaction with Kpns and movement of LRRC59 along the ER and NE membranes PMID: 22321063
  49. [review] In this review, the authors discuss recent and ongoing research into the role of fibroblast growth factor and transforming growth factor-beta in the etiopathogenesis of craniosynostosis. PMID: 21806346
  50. Plasticity in interactions of fibroblast growth factor 1 (FGF1) N terminus with FGF receptors underlies promiscuity of FGF1. PMID: 22057274
  51. In the present study, a novel hybrid protein combining LL-37 with haFGF was designed. PMID: 21963769
  52. Acidic fibroblast growth factor (FGF) potentiates glial-mediated neurotoxicity by activating FGFR2 IIIb protein. PMID: 21990352
  53. FGFR3c, critical for bone development, elicit a similar response to Fgf1 and Fgf2 at low, but not at high, concentrations; results demonstrate the versatility of FGFR3c response to fgf1 and fgf2 and highlight the complexity in fgf signaling. PMID: 21894939
  54. FGF1 is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
  55. FGF1 is implicated in the occurrence of cleft palate. PMID: 21462296
  56. A unique role for hematopoietic stem cell cytokines in vascular tube morphogenesis and sprouting and a novel upstream priming role for VEGF and FGF to facilitate the action of promorphogenic hematopoietic cytokines. PMID: 21239704
  57. The world-wide largest angiogenesis gene therapy trial in 525 patients suffering from critical limb ischemia and treated with the non-viral gene construct for fibroblast growth factor (NV1FGF) or placebo was negative for all endpoints. PMID: 21448824
  58. Fibroblast growth factor 1/fibroblast growth factor receptor 3 (FGF1/FGFR-3) signaling are mediators leading to the increased migration and invasion. PMID: 21356388
  59. Hydrogen/deuterium exchange rates were measured on the urea-denatured hFGF-1 to obtain the information with regard to the persistent residual interaction(s) in the unfolded hFGF-1. PMID: 21190667
  60. Data suggest that an FGFR1alpha-to-FGFR1beta isoform switch and increased FGF1-induced activation of FGFR1beta may result in a proliferative advantage that plays a key role during bladder tumor progression. PMID: 20889570
  61. This article describes a new, rapid procedure based on Taq polymerase for the precise assembly of DNA oligonucleotides to yield the complete human fibroblast growth factor 1 (FGF1) gene. PMID: 20706664
  62. The FGF1 -1385A/G polymorphism is shown to not influence the risk of developing adenomyosis but is associated with the risk of developing endometriosis. PMID: 20504870
  63. S100A13 promotes the release of FGF-1 protein, but does not affect the transportation of FGF-1 protein in human umbilical vein endothelial cells PMID: 20863990
  64. Our data suggested no association between the FGF1 -1385 C>T polymorphism and AD risk in Chinese Han population. PMID: 20298684
  65. Data show that a significant increase in plasma SDF-1alpha, and an immediate decrease in plasma VEGF and FGF levels after chemotherapy. PMID: 20072657
  66. RFX1 may negatively regulate the self-renewal of GBM-SCs through modulating FGF-1B and FGF1 expression levels by binding the 18-bp cis-elements of the F1B promoter. PMID: 20189986
  67. Studies indicate communication between tumor cells and their microenvironment is through polypeptide growth factors EGF, FGF, PDGF and receptors for these growth factors. PMID: 20036812
  68. The hematopoietic properties of yolk sac may be dependent on signaling through FGF receptors and FGF1 plays an important role in hematopoietic stem cell homeostasis. PMID: 19112917
  69. NMR characterization of copper and lipid interactions of the C2B domain of synaptotagmin I-relevance to the non-classical secretion of the human acidic fibroblast growth factor (hFGF-1). PMID: 19835837
  70. The level of FGF-1 in osteosarcomas was significantly higher than in chondrosarcomas. PMID: 20027339
  71. Results describe a series of mutations in fibroblast growth factor 1 at crystal contacts designed to both reduce side-chain entropy and replace the solvent-mediated interface with direct side-chain contacts to improve neutron diffraction. PMID: 19923735
  72. Alternative type I and I' turn conformations in the beta8/beta9 beta-hairpin PMID: 11847269
  73. possible role of fibroblast growth factors in expression of genes of the plasminogen activator system in breast fibroblasts PMID: 12008951
  74. bFGF and aFGF may suppress endothelial-dependent monocyte recruitment and thus have an anti-inflammatory action during angiogenesis in chronic inflammation but inhibit the immunoinflammatory tumor defense mechanism PMID: 12057924
  75. identification of rare partially unfolded states in equilibrium with the native conformation PMID: 12118009
  76. Recombinant human FGF-1 binds to both the catalytic alpha-subunit and to the regulatory beta-subunit of CK2. A correlation between the mitogenic potential of FGF-1 mutants and their ability to bind to CK2 alpha was observed. PMID: 12145206
  77. Maintenance of the human umbilical vein endothelial cell phenotype and differentiation into smooth muscle-like cells are reciprocally controlled by fibroblast growth factor-1 and activin A. PMID: 12417591
  78. The mitogenic acitivity of AFGF was inhibited by the synthetic peptide Ac-ValTyrMetSerProPhe-NH2. PMID: 12440521
  79. The conformational stability and dynamics of FGF1 have been measured on the basis of amide proton-exchange kinetics, monitored by two-dimensional NMR spectroscopy. PMID: 12484774
  80. No associations of intracranial aneurysm and FGF1 were found. PMID: 12750963
  81. According to phase of menstrual cycle, endometrial stromal cells from endometriosis patients in late proliferative phase had higher bFGF mRNA values and lower 1B FGF-antisense mRNA compared with controls PMID: 12788899
  82. Binding studies demonstrated no high-affinity interaction of FGF-1 with fibrinogen or fibrin; unlike FGF-2, FGF-1 can support vascular endothelial cell growth only if continually present in soluble form. PMID: 12871334
  83. An alternative core packing group, involving a set of five positions, has been introduced into human acidic FGF-1 PMID: 14627732
  84. FGF1 transcriptionally induces membrane type-1 matrix metalloproteinase expression in a prostate carcinoma cell line. PMID: 14673954
  85. Data show that mouse and human fibroblast growth factor 1 (FGF-1) internal ribosome entry sites show similar activity profiles, and have a conserved structural domain at both the nucleotide sequence and RNA structure levels. PMID: 15314170
  86. Promoter polymorphism and genetic susceptibility for Alzheimer's disease PMID: 15358178
  87. 1.10-A atomic resolution X-ray structure of human fibroblast growth factor 1 (FGF-1), a member of the beta-trefoil superfold, has been determined PMID: 15382229
  88. FGF-1 is a key human adipogenic factor, involved in fat tissue growth. PMID: 15561939
  89. residues flanking the the beta4/beta5 and beta8/beta9 beta-hairpin turns of human acidic fibroblast growth factor are a primary structural determinant of the conformation within the turn PMID: 15632285
  90. FGF-1 contains a second putative NLS (NLS2), which is located near the C-terminus. It is a bipartite NLS consisting of two clusters of lysines separated by a spacer of 10 amino acids. PMID: 15835896
  91. FGF-1 regulates HO-1 expression through Nrf2 PMID: 15870071
  92. A recombinant nonmitogenic form of aFGF (nhaFGF) was purified and the mitogenic activity of the purified nhaFGF was decreased dramatically comparable to that of the wild-type aFGF (haFGF). PMID: 15882952
  93. Data indicate that after endocytosis, fibroblast growth factor receptor (FGFR)4 and its bound ligand, FGF1, are sorted mainly to the recycling compartment, whereas FGFR1-3 with ligand are sorted mainly to degradation in the lysosomes. PMID: 16091423
  94. FGF1 dimerization upon heparin was favored over monomeric interactions even when a large excess of saccharide was present PMID: 16219767
  95. FGF-1 might have both autocrine and paracrine functions in renal inflammation PMID: 16316338
  96. A type I 4:6 turn at turn 1 of FGF-1 appears essential for efficient mitogenic function. PMID: 16355415
  97. Data suggest that minor changes of local kynurenic acid concentration may modulate fibroblast growth factor-1 release, cell proliferation, and ultimately tissue damage in different pathological conditions. PMID: 16392031
  98. Two molecules of suramin appear to bind simultaneously to one molecule of hFGF-1 with high affinity PMID: 16411766
  99. Hsp90 is required for translocation of FGF-1 and FGF-2 across the endosomal membrane PMID: 16495214
  100. FGF-1 can have an antifibrogenic role, inducing apoptosis of fibroblasts and inhibiting myofibroblast differentiation PMID: 16766579

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Subcellular Location Secreted, Cytoplasm, Cytoplasm, cell cortex, Cytoplasm, cytosol, Nucleus
Protein Families Heparin-binding growth factors family
Tissue Specificity Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.
Database Links

HGNC: 3665

OMIM: 131220

KEGG: hsa:2246

STRING: 9606.ENSP00000338548

UniGene: Hs.483635

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