Code | CSB-E04546h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human aFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 91 | ||||
Range % | 86-95 | |||||
1:2 | Average % | 102 | ||||
Range % | 97-107 | |||||
1:4 | Average % | 91 | ||||
Range % | 85-97 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-103 |
The recovery of human aFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
EDTA plasma (n=4) | 97 | 90-100 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
1000 | 2.471 | 2.498 | 2.485 | 2.319 | |||
500 | 1.827 | 1.868 | 1.848 | 1.682 | |||
250 | 1.369 | 1.366 | 1.368 | 1.202 | |||
125 | 0.898 | 0.861 | 0.880 | 0.714 | |||
62.5 | 0.474 | 0.461 | 0.468 | 0.302 | |||
31.2 | 0.334 | 0.352 | 0.343 | 0.177 | |||
15.6 | 0.234 | 0.236 | 0.235 | 0.069 | |||
0 | 0.164 | 0.168 | 0.166 |
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This human FGF1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human FGF1 in serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the FGF1 levels in the sample and is measured at 450 nm through a microplate reader.
FGF1 provides additional protection against apoptosis and promotes cell survival within cells. FGF1 has been shown to play an important role in regulating the fate of bone marrow stromal cells (BMSCs) by suppressing osteogenesis and promoting adipogenesis. FGF1 expression is elevated during the formation of a cartilaginous callus in fracture, especially in fibroblast-like mesenchymal cells. FGF1 is considered a catabolic factor through down-regulating of CCN2 by interaction and enhancing the degradation of cartilaginous ECM by MMP13. FGF1 also stimulates lung epithelial cell proliferation and airway bud formation.
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