Recombinant Human Glial cell line-derived neurotrophic factor protein(GDNF) (Active)

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Code CSB-AP002881HU
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of >5.0x107 IU/mg.
Target Names GDNF
Uniprot No. P39905
Research Area Neuroscience?
Alternative Names Astrocyte derived trophic factor ; Astrocyte derived trophic factor 1; Astrocyte-derived trophic factor; ATF 1 ; ATF 2; Atf; ATF1; ATF2; gdnf; GDNF_HUMAN; Glial cell derived neurotrophic factor; Glial Cell Line Derived Neurotrophic Factor; Glial cell line-derived neurotrophic factor; Glial derived neurotrophic factor; HFB1 GDNF; hGDNF; HSCR3
Species Homo sapiens (Human)
Source E.Coli
Expression Region 78-211aa
Complete Sequence SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI
Mol. Weight 15.1 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 m filtered 1 × PBS, pH 7.4, with 0.05 % Tween-20
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
Gene References into Functions
  1. Data show that GDNF promotes angiogenesis through demethylation of the FMOD promoter in human glioblastoma. PMID: 30176167
  2. the inverse relationship between GFRalpha1 and C-Ret, as knocking down C-Ret led to increases in GFRalpha1 expression. PMID: 29018141
  3. This study showed that the GDNF levels in preterm newborns were higher in cord blood and lower in CSF as compared to term newborns. PMID: 29031644
  4. We thus confirmed the role of GDNF as an adaptive survival factor, and its alteration appears to have a key role in nephrocalcinosis. We also discovered that, in GDNF-silenced cells, death occurs in a programmed but caspase-independent manner. PMID: 29208768
  5. Results suggested that while alterations at 5:37812784 T > A and 5:37812782 T > A sites related with higher GDNF serum levels and less functionality, and alterations at rs62360370 G > A 3'UTR SNP of GDNF associated with higher severity and lower functionality. However, alterations at both rs2075680 C > A and rs79669773 T > C SNPs affect neither GDNF serum levels nor severity and functionality in bipolar disorder. PMID: 28891527
  6. To our knowledge, this is the first study which correlates GDNF levels with metabolic parameters. Our results show no differences in GDNF serum level between schizophrenia, a first depressive episode, and healthy controls. GDNF serum level did not correlate with metabolic parameters except for total cholesterol in depression. PMID: 28689143
  7. The results suggest an interaction between NGF, GDNF and MMP-9 during the transition to malignancy in prostate cancer (PC). Also this interaction may involve in regulating PC cell differentiation, tumor invasion, progression, and the agressiveness of PC. PMID: 28237042
  8. Study also demonstrates that the interaction between GDNF and proN-cadherin activates specific intracellular signaling pathways; furthermore, GDNF promoted the secretion of matrix metalloproteinase-9 (MMP-9), which degrades the ECM via proN-cadherin. PMID: 28212546
  9. Protective effect of GDNF-engineered amniotic fluid-derived stem cells on the renal ischaemia reperfusion injury in vitro. PMID: 29114949
  10. GDNF has a role in lower than expected motor development, but while IL-1beta and CXCL8/IL-8 values were higher in the group with typical motor development among preterm neonates PMID: 28553016
  11. These findings provide preliminary evidence that silencer II hypermethylation in the gdnf promoter II may underlie high gene transcription in high-grade glioma cells. PMID: 26232065
  12. there is a decrease in epidermal GDNF and GFRalpha-1 protein expression in normal human skin with ageing PMID: 27346872
  13. In SOD1(G93A) spinal cords, we verified a strict correlation in the expression of the TNFalpha, TNFR1 and GDNF triad at different stages of disease progression. Yet, ablation of TNFR1 completely abolished GDNF rises in both SOD1(G93A) astrocytes and spinal cords, a condition that accelerated motor neuron degeneration and disease progression PMID: 27288458
  14. In functional dyspepsia patients, duodenal expression of GDNF protein was significantly increased compared with controls. GDNF was localized in enteric glial cells, eosinophils, and epithelial cells. PMID: 27718082
  15. Suppression of miR-383 may increase the therapeutic potential of human bone-marrow-derived MSCs in treating spinal cord injury via augmentation of GDNF protein levels. PMID: 28365701
  16. Our results suggest that GDNF rs3096140 might be involved in the genetic background of smoking, independent of anxiety characteristics. PMID: 27994179
  17. No correlations between the levels of serum neurotrophins and the severity of ADHD were observed. These results suggest that elevated serum GDNF and NTF3 levels may be related to ADHD in children. PMID: 27561780
  18. BDNF and GDNF interact with the 5-HT-system of the brain through feedback mechanisms engaged in autoregulation PMID: 28320272
  19. Study shows no correlation between GDNF rs884344 and rs3812047 polymorphisms and subjects with tinnitus. PMID: 27180191
  20. These results demonstrate the pivotal role of GDNF as well as the PI3K/Akt pathway, but not the MAPK pathway, in the prevention of diabetesinduced neuronal apoptosis in the hippocampus. PMID: 26549420
  21. Increased plasma levels of GDNF were found in untreated attention deficit hyperactivity disorder patients. PMID: 25753832
  22. GDNF levels were lower in the ECT responders compared with pre-ECT levels PMID: 25354171
  23. The GDNF polymorphism rs3096140 is associated with Tourette syndrome. PMID: 26096985
  24. GDNF levels were significantly higher in mania and lower in schizophrenia compared to healthy controls. BDNF levels were negatively correlated to illness severity scores in affective episodes PMID: 25543333
  25. Data indicate that glial cell-derived neurotrophic factor (GDNF) was down-regulated in the medullary sponge kidney (MSK) cells. PMID: 25692823
  26. Osteogenic features of MSCs from ossification of yellow ligament patients are promoted by un-methylated GDNF. PMID: 25913759
  27. The glial cell line-derived neurotrophic factor(GDNF)-induced growth and differentiation of cultured enteric neurons is paralleled by increased expression of SNAP-25 and formation of synaptic vesicles reflecting enhanced synaptogenesis. PMID: 25655772
  28. improvement in behavioral performance (open-field and grip-strength tests), as well as increased life-span was observed in rodents treated with NCAM-VEGF or NCAM-GDNF co-transfected cells PMID: 25619885
  29. Data indicate the glial cell derived neurotrophic factor (GDNF) signaling pathway as a potential target for improving responses to conventional genotoxic therapeutics. PMID: 25575823
  30. In major depressive disorder patients, serum GDNF levels were lower compared to controls. PMID: 24899094
  31. the PTX-sensitive alpha-subunit of G proteins, Go1 and Gi3, plays an important role in a monoamine-independent production of GDNF evoked by amitriptyline PMID: 25869129
  32. These results suggest that D2R activation induces a Gbetagamma- and extracellular signal-regulated kinase 1/2-dependent increase in the level of Zif268, which functions to directly up-regulate the expression of GDNF. PMID: 23373701
  33. An increased risk of HD[Heroine Dependence] and depression was associated with rs2910709 T/T genotype and rs884344 C allele, respectively, suggesting GDNF is a novel susceptibility gene for depression and HD. PMID: 24022000
  34. histone H3K9 hyperacetylation in promoter II of the gdnf gene might be one of the reasons for its abnormal high transcription in glioma cells. PMID: 24619502
  35. Study found association between single nucleotide polymorphisms in the regulatory sites of Glial Derived Neurotrophic Factor (GDNF) and bipolar disorder. PMID: 24997227
  36. Transgenic expression of human glial cell line-derived neurotrophic factor from integration-deficient lentiviral vectors is neuroprotective in a rodent model of Parkinson's disease. PMID: 24635742
  37. No differences in plasma BDNF, NGF, NT3, NT4 and GDNF were found between autism spectrum disorders and control. PMID: 25275256
  38. GDNF expression was upregulated upon radiation therapy in submandibular glands. PMID: 25036711
  39. This hypothesis offers a dramatically different explanation for the etiology of sporadic Parkinson's disease as a manifestation of acquired resistance to GDNF. PMID: 25448159
  40. serum levels of GDNF may be an unsuitable biomarker for schizophrenia, although it may be associated with working memory in healthy controls and the pathophysiology of attention deficits in schizophrenia. PMID: 24861509
  41. GDNF is a novel candidate gene of anxiety. PMID: 24324616
  42. GDNF increases the metastasis and migration of colon cancer cells PMID: 24165321
  43. Transgenic GDNF+/- mice, not wild-type, show increased kidney glomerular damage with a low nephron number. PMID: 24247178
  44. These findings collectively demonstrate that GFRalpha1 released by nerves enhances perineural invasion through GDNF-RET signaling and that GFRalpha1 expression by cancer cells enhances but is not required for it. PMID: 24778213
  45. Changes in transcriptional factor binding capacity are due to changes in promoter region methylation and might be the underlying mechanism for aberrantly high gdnf expression in glioma. PMID: 23606280
  46. Glial cell-derived neurotrophic factor has a regulatory effect on cell migration in oral squamous cancer. PMID: 24070603
  47. The novelty of the study lies in its large number of exclusively adolescent depression patients showing significant reduction of BDNF, NGF and GDNF serum levels as compared to controls. A gender bias with much reduction in female has also been recorded. PMID: 23769609
  48. inducible lentivirus-mediated hGDNF gene delivery system may provide useful tools for basic research on gene therapy for chronic neurological disorders such as Parkinson's disease PMID: 23717608
  49. Results indicate GDNF-RET signaling as a rational therapeutic target to combat or delay the onset of aromatase inhibitor resistance in breast cancer. PMID: 23650283
  50. Human GDNF protected crayfish neurons and glia from photodynamic injury. PMID: 22847529
  51. Data suggest that the inducible expression of GDNF in neural stem cells (hNSCs) may provide a system for the controllable delivery of GDNF in patients with neurodegenerative diseases. PMID: 23269553
  52. Our results indicate an age-associated reduction in serum GDNF levels in patients with major depressive disorder. PMID: 23022671
  53. Compared with resting EMPhis, tEMPhis secreted higher levels of glial-derived neurotrophic factor (GDNF), inducing phosphorylation of RET and downstream activation of extracellular signal-regulated kinases (ERK) in PDA cells. PMID: 22971345
  54. Proliferation of endogenous neural stem cells in the subventricular and subgranular zones of the human hippocampus dentate gyrus is activated by cerebral infarction and causes increases in GDNF expression. PMID: 20426902
  55. analysis of neuroprotection by GDNF in the ischemic brain [review] PMID: 22670840
  56. Direct midbrain delivery of AAV2-GDNF resulted in extensive anterograde transport to multiple brain regions PMID: 22019719
  57. results indicate that the regulation of GDNF secreted from pericytes may therefore be a novel therapeutic strategy to modify the BBB or BNB functions and promote brain or peripheral nerve regeneration PMID: 22002662
  58. [review] The vgf gene is induced in vivo by neurotrophins including nerve growth factor (NGF), brain derived growth factor (BDNF) and glial derived growth factor (GDNF), by synaptic activity and by homeostatic and other stimuli. PMID: 21621608
  59. GDNF restores human blood-nerve barrier function via RET tyrosine kinase-mediated cytoskeletal reorganization PMID: 22326552
  60. There is an autocrine anti-apoptotic loop in enteric glia cells which is operative in Crohn's disease and dependent of GDNF. PMID: 22251670
  61. After a single intracerebral injection a glial cell line-derived human GDNF expression plasmid administered to rat striatum as a compacted DNA nanoparticle generates long-term hGDNF expression at biologically active levels 300-400% higher than baseline. PMID: 21839809
  62. Peripheral levels of GDNF negatively correlate with different mood states in bipolar disorder, reinforcing the involvement of neurotrophic factors in its pathophysiology. PMID: 21820487
  63. This study provides evidence for a direct, receptor-mediated effect of GDNF on osteoblasts highlighting a novel role for GDNF in bone physiology. PMID: 22142702
  64. Identification of novel GDNF isoforms and cis-antisense GDNFOS gene and their regulation in human middle temporal gyrus of Alzheimer disease PMID: 22081608
  65. results showed that in TGW cells, GDNF increased SPHK1 transcription, leading to the production and secretion of S1P PMID: 21769916
  66. Findings, together with the detection of sequence variants in PROKR1, PROK1 and PROKR2 genes associated to HSCR and, in some cases in combination with RET or GDNF mutations, provide evidence to consider them as susceptibility genes for HSCR. PMID: 21858136
  67. overexpression of SorLA could enhance the regulated secretion of the GDNF prodomain-GFP fusion protein, suggesting that the prodomain of GDNF is responsible for its regulated secretion. PMID: 21994944
  68. patients Methamphetamine dependent were divided in two groups having of multi-drug vs MAP-only users, we detected significant association between the two groups and tagging SNP rs2910704. GDNF likely related to severity of MAP use in Japanese population PMID: 21514351
  69. S100B protein, BDNF, and GDNF are present in all samples of human milk, and they may be responsible for the long term effects of breast feeding. PMID: 21738758
  70. These results suggest that genomic alteration of RET or GDNF is not a major mechanism leading to renal agenesis and other severe kidney development defects. PMID: 21490379
  71. findings suggest that late-onset depression (LOD) patients in acute phase have extensive impairments of cognitive function, and higher plasma GDNF might be involved in the pathogenesis of LOD, which may be associated with the cognitive dysfunction in LOD. PMID: 21524799
  72. FSH-induced Nur77 regulates the expression of GDNF in Sertoli cells to stimulate the proliferation of A spermatogonia in vitro. PMID: 21726557
  73. GDNF-mediated c-RET kinase activation is associated with the pathogenesis of malignant melanoma PMID: 20422010
  74. investigation of regulation of expression of GDNF (and BDNF) by granulosa cells; GDNF (and BDNF) promote oocyte maturation PMID: 21227937
  75. Limbal progenitor cell-produced neurotrophic factor GDNF suppresses IL-17-mediated inflammation via NF-kappaB signaling pathway. PMID: 20936708
  76. Up-regulation of GDNF and RET ligand-receptor interaction might participate in the glucose-induced cancer progression. PMID: 20960036
  77. Human bone-marrow derived mesenchymal stem cells, genetically modified to secrete GDNF, hold potential as an allogeneic or autologous stem cell therapy for Parkinson's disease. PMID: 20544825
  78. Syndecan-3 may directly transduce Glial cell line-derived neurotrophic factor (GDNF) family ligands (GFL) signals or serve as a coreceptor, presenting GFLs to the signaling receptor RET. PMID: 21200028
  79. GDNF synthesis occurs in peritubular cells in testes of normal, subfertile, & infertile men. GDNF is localized in specific testicular compartments. Role of GDNF in spermatogonial stem cell niche was investigated. PMID: 20601681
  80. Injection of an adenoviral vector harboring human GDNF protein into rat sensorimotor cortex one week prior to a controlled cortical impact results in significant neuroprotection; however, overall recovery is not sustained. PMID: 20479525
  81. GDNF gene levels in the esophageal mucosa were significantly increased in patients with erosive esophagitis compared with asymptomatic patients and healthy controls PMID: 20518854
  82. Studies indicate that three genes within the same GDNF-RET signaling pathway have been implicated in achalasia: GDNF, RET, and SPRY2. PMID: 20700745
  83. Identify GDNF gene sequence variations in patients with medullary sponge kidney disease and suggest a role for this gene in the pathogenesis of some cases of the disease. PMID: 20448065
  84. GDNF variants associated with the apnea-hypopnea index as a continuous trait, and OSA as a dichotomous trait in European Americans PMID: 20538960
  85. Both (alpha)pro-GDNF and (beta)pro-GDNF isoform precursors are secreted from human cultured cells. PMID: 20739562
  86. The GDNF protein was detected in oocytes of all samples. Granulosa cells (GCs) stained for GDNF in a portion of fetal samples and in all samples from girls/women. PMID: 19896648
  87. Report a procedure for the production and purification of human bioactive glycosylated GDNF using a mammalian cell expression system. PMID: 19825405
  88. Report GDNF transport across the blood brain barrier by re-engineering the neurotrophin as a fusion protein with a genetically engineered monoclonal antibody against the human insulin receptor. PMID: 19741036
  89. a candidate modifier in a type 1 neurofibromatosis (NF1) enteric phenotype PMID: 11565554
  90. GDNF and NRTN are new neuromodulators that regulate the development of the neuromuscular synapse PMID: 11790765
  91. Two GDNF mutations identified in Hirschsprung disease patients have reduced binding affinity for the receptor PMID: 11823451
  92. GDNF may play a role in the development of intrahippocampal circuitry and in neuronal function and maintenance throughout life. PMID: 11844480
  93. GDNF mRNA-containing neurons were found in normal neonate and adult human hippocampus. The data suggest a role for GDNF in neuronal function throughout life. PMID: 11844483
  94. Hirschsprung associated GDNF mutations do not prevent RET activation PMID: 11973622
  95. The gene expression of this protein was studied in the developing human tooth. PMID: 12397373
  96. involvement of heparan sulphate glycosaminoglycan in GDNF signalling PMID: 12414995
  97. GDNF upregulates expression and enzymatic activity of MMP-9 through different signaling pathways. GDNF modulates MMP-9 expression and activation, and this may promote pancreatic cancer invasion. PMID: 12947332
  98. This study have performed, in an Italian sample, an association study on 3' UTR (AGG)n repeat in GDNF gene. Our results have evidenced a difference in the allele frequencies between patients and controls. PMID: 15003293
  99. GDNF over-expression in myo-GDNF transgenic mice enhances reinnervation, but at the expense of both neurofilament integrity and functional reliability. PMID: 15106825
  100. A locally delivered human GDNF-expressing lentiviral vector allows partial protection of motoneurons in the facial nucleus of SOD1(G93A) transgenic mice, whereas it does not prevent loss in the spinal cord. PMID: 15207271

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Involvement in disease Hirschsprung disease 3 (HSCR3); Congenital central hypoventilation syndrome (CCHS); Pheochromocytoma (PCC)
Subcellular Location Secreted
Protein Families TGF-beta family, GDNF subfamily
Tissue Specificity In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic
Database Links

HGNC: 4232

OMIM: 171300

KEGG: hsa:2668

STRING: 9606.ENSP00000409007

UniGene: Hs.248114

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