Recombinant Mouse Hepatitis A virus cellular receptor 2 homolog(Havcr2),partial (Active)

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Code CSB-AP005341MO
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined by its ability to bind Human Galectin 9 in functional ELISA is less than 20 ug/ml.
Target Names Havcr2
Uniprot No. Q8VIM0
Research Area Immunology
Alternative Names Havcr2; Tim3; Timd3; Hepatitis A virus cellular receptor 2 homolog; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3; CD antigen CD366
Species Mus musculus (Mouse)
Source Mammalian cell
Expression Region 20-191aa
Complete Sequence RSLENAYVFEVGKNAYLPCSYTLSTPGALVPMCWGKGFCPWSQCTNELLRTDERNVTYQKSSRYQLKGDLNKGDVSLIIKNVTLDDHGTYCCRIQFPGLMNDKKLELKLDIKAAKVTPAQTAHGDSTTASPRTLTTERNGSETQTLVTLHNNNGTKISTWADEIKDSGETIR
Mol. Weight 46.3 kDa
Protein Length Extracellular Domain
Tag Info C-terminal FC-tagged
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand
Gene References into Functions
  1. TIM-3 expression was predominantly localized to myeloid cells in both human and murine tumors PMID: 29316433
  2. Here, the authors demonstrate that Tim-3 inhibits macrophage phagocytosis of Listeria monocytogenes by inhibiting the nuclear erythroid 2-related factor 2 (Nrf2) signaling pathway and increases bacterial burden. PMID: 28205579
  3. Studied role of Tim-3 role's in T cell activation using a chronic disease model. Results showed a major function of Tim-3 is to enhance T cell activation during either acute or chronic viral infection, and that Tim-3 is not required for the development of T cell exhaustion. PMID: 29463725
  4. the data suggested that Tim-3 played a crucial role in the macrophage polarization and brain inflammation following intracerebral hemorrhage PMID: 29107214
  5. Tim-3 might contribute to successful pregnancy by restraining Th1 bias PMID: 29083087
  6. synergism in cell death by Caspase-1- and RipK3 resulted in restriction of PD-1 and TIM3 expression on primed CD8(+) T cells, which promoted the survival of activated CD8(+) T cells. PMID: 28686578
  7. these findings reveal a critical role for Tim-3-Gal-9 signaling-mediated immunoregulation by pNK cells in maternal-fetal immune tolerance and suggest that Tim-3 abundance on pNK cells is a potential biomarker for recurrent miscarriage diagnosis. PMID: 28951537
  8. Tim-3 and PD-1 pathways play critical roles in regulating CD8(+) T cell function and maintaining normal pregnancy. PMID: 28331165
  9. CB2R may have a crucial neuroprotective role following HI insult through the modulation of the inflammatory-related HIF-1alpha/TIM-3 signalling pathway in microglia PMID: 28253997
  10. Our results show that Tim-3 is a critical negative regulator of NLRP3 inflammasome and provides a potential target for intervention of diseases with uncontrolled inflammasome activation. PMID: 28799242
  11. demonstrated that both IL-10 and TGF-beta upregulated TIM-3 surface expression on dendritic cells via a common signaling pathway that involved sequential activation of c-Src and Bruton's tyrosine kinase PMID: 27439518
  12. Methylation level of the TIM-3 promoter gradually decreased after each round of T-cell polarization, and this decrease was inversely correlated with TIM-3 expression. PMID: 26890332
  13. in mice, TIM-3 is not essential for development of HDM-induced acute or chronic allergic airway inflammation, although it appears to be involved in reduced lymphocyte recruitment during HDM-induced chronic allergic airway inflammation. PMID: 27209052
  14. Authors found that a CD8(+) T-cell population with age-associated exhaustion was distinguishable by its expression of Tim-3. PMID: 26750587
  15. TIM4 binds TIM3 on the surface of polarized Th1 cells to induce Th1 cell apoptosis, which may contribute to the development of Th2-dominant immune disorders. PMID: 26403707
  16. decreases immunological rejection during composite tissue allotransplantation PMID: 26561949
  17. The Gal-9/Tim-3 signal is important for the regulation of decidua NK cells function, which is beneficial for the maintenance of a normal pregnancy. PMID: 25578313
  18. TIM-3 expression by NK cells and gamma/delta T cells is similar in the peripheral and decidual immune cells from pregnant mice. PMID: 26278059
  19. Tim-3 acts at a receptor-proximal point to enhance Lyn kinase-dependent signaling pathways that modulate both immediate-phase degranulation and late-phase cytokine production downstream of FcepsilonRI ligation. PMID: 26598760
  20. Our data indicate that Tim-3 expression on NK cells is regulated by T-bet, and that Tim-3 levels correlate with advanced stages of gastric cancer PMID: 26214042
  21. TIM-3 plays a role in regulating the uterine natural killer cells and contributes to the maintenance of tolerance at the feto-maternal interface. PMID: 25897749
  22. TIM-3 is highly expressed in hypoxic brain regions of a mouse cerebral hypoxia-ischaemia (H/I) model. TIM-3 is distinctively upregulated in activated microglia and astrocytes in a HIF-1-dependent manner. PMID: 25790768
  23. These data identify an IL-27/NFIL3 signalling axis as a key regulator of effector T-cell responses via induction of Tim-3, IL-10 and T-cell dysfunction. PMID: 25614966
  24. CD4+ T cell-dependent "negative" TIM-3 costimulation is essential for hepatic homeostasis and resistance against IR stress in OLTs PMID: 25676534
  25. In DSS colitis, Tim-3 inhibited the polarization of pathogenic pro-inflammatory M1 macrophages, while Tim-3 downregulation or blockade resulted in an increased M1 response. Tim-3 effect on macrophage response may be TLR4 dependent. PMID: 26208474
  26. These data suggest that Tim-3 and IFN-gamma may play a regulatory role in T. gondii-infected pregnant mouse model. PMID: 25270237
  27. Gal-9, a natural TIM-3 ligand, was produced primarily by and released from ischemia-reperfusion-stressed hepatocytes, both in vivo and in vitro. PMID: 25450716
  28. HCC conditioned medium or transforming growth factor-beta fostered Tim-3 expression and the alternative activation of macrophages. PMID: 25608525
  29. Tim-3 induces Th2-biased immunity and macrophage activation during Schistosoma japonicum infection. PMID: 25987707
  30. The Tim-3 pathway plays an inhibitory role in natural killer cell function. PMID: 25337993
  31. Specific antigen-presenting cell subsets and the inhibitory receptor Tim-3 may contribute to CD8+ T cell impairment in metapneumovirus pathogenesis. PMID: 25653440
  32. Tim-3 modulates microglia activity and regulates the interaction of microglia-neural cells. PMID: 25557503
  33. The TIM-3 pathway ameliorates Theiler's murine encephalomyelitis virus-induced demyelinating disease. PMID: 24486565
  34. CEACAM1 serves as a heterophilic ligand for TIM-3 that is required for its ability to mediate T-cell inhibition, and this interaction has a crucial role in regulating autoimmunity and anti-tumour immunity PMID: 25363763
  35. Tim-3 is widely involved in the physiopathology of sepsis by regulating innate and adaptive immune responses. Blockade of the Tim-3 pathway or decreased Tim-3 expression may exacerbate sepsis by increasing immune deviation and immunosuppression in vivo. PMID: 24945079
  36. Tim-3 signaling pathway acts as a novel negative mediator in LPS-induced endotoxic shock and could be a potential therapeutic target for the treatment of sepsis. PMID: 24561184
  37. These results demonstrate an essential role for Btk-c-Src signaling in TIM-3-induced DC suppression. PMID: 25172495
  38. The increased expression of IL-17 and Tim-3 in BALF. PMID: 23904364
  39. Tim-3/galectin-9 pathway plays a critical role in the homeostasis of hepatic Tregs through the elimination induction in Teffs and the inhibition of IFN-gamma release. PMID: 24333756
  40. Our findings demonstrate that Tim-3 plays an important role in brain inflammation after intracerebral hemorrhage PMID: 24289479
  41. Data indicate that the absence of T cell Ig and mucin domain (Tim) 3 (Tim-3) expression impairs CD8 T cell responses to Listeria monocytogenes infection. monocytogenes PMID: 24567532
  42. results indicate that the Tim-3 pathway is highly involved in the regulation of asthma; targeting Tim-3 by siRNA may hold therapeutic potential in preventing the development of allergic asthma PMID: 23232962
  43. These data indicate that Tim-3 may be involved in the process of toxoplasmic encephalitis in mice infected with the Toxoplasma gondii Pru strain. PMID: 23595213
  44. Anti-Tim-3 treatment augments atherosclerosis lesion development, accompanied by an increase in the number of monocytes/macrophages and CD4(+) T cells and by decreased regulatory T cells and regulatory B cells. PMID: 23990206
  45. These results indicate that the therapeutic efficacy of targeting Tim-3 in experimental autoimmune encephalomyelitis is dependent on the nature of the effector T cells contributing to the disease. PMID: 23562810
  46. Up-regulated expression of Tim-3 on T cells after burn injury plays an important role in the development and maintenance of burn-induced T cell immune suppression. PMID: 22038354
  47. In the liver Tim-3+ natural killer {NK)T cells are in an activated state, and Gal-9 directly induces Tim-3+ NKT cell apoptosis and contributes to the depletion of NKT cells in diet-induced steatosis. PMID: 23296703
  48. T-cell Ig mucin-3 (Tim-3) and its ligand, galectin 9 (Gal-9), were significantly decreased in dextran sodium sulfate-induced colitis PMID: 23117395
  49. Deletion of the terminal cytoplasmic domain of Tim3 potentiates its ability to downregulate cytotoxic lymphocytes (Tc1) inflammation; this enhanced Tim3 activity is associated with decreased phosphorylation of the T cell receptor-CD3zeta-chain. PMID: 22875804
  50. TIM-3 circumvents the stimulatory effects of nucleic acids in tumor immunity by interactding with HMGB1 to interfere with the recruitment of nucleic acids into dendritic cell endosomes PMID: 22842346
  51. These studies are the first to show that the absence of Tim-3/gal-9 pathway interactions augments systemic graft versus host disease and regulatory T-cells contributute to it. PMID: 22677125
  52. the number and frequencey of FoxP3, TIM-3, PD-1 expressing Treg cells, present during allograft response, peaks at the time of graft rejection. PMID: 22684103
  53. Soluble Gal-9 interacts with Tim-3 expressed on the surface of activated CD4(+) T cells. This leads to down-regulation of HIV-1 coreceptors and up-regulation of the cyclin-dependent kinase inhibitor p21. PMID: 22438246
  54. Galectin-9 regulates T helper cell function independently of Tim-3 PMID: 21187321
  55. The Tim-3 and PD-L1-mediated signaling can significantly reduce pathologies in the upper genital tract without suppressing immunity against chlamydial infection. PMID: 22168579
  56. Tim-3 expression was increased in Helicobacter pylori infection. PMID: 21923683
  57. augmented expression of Tim-3 may play an important role in the pathogenesis of ischemic stroke by regulation of proinflammatory cytokines. PMID: 21519864
  58. host homeostatic mechanism mediated by Gal-9/Tim-3 interaction on CD8(+) T cells can influence the outcome of HSV-1 latent infection. PMID: 22021615
  59. we identified a unique phenotype of exhausted T cells in mice with advanced acute myelogenous leukemia; this phenotype is characterized by the coexpression of Tim-3 and PD-1 on CD8(+) T cells in the liver PMID: 21385853
  60. Galectin-9 and Tim-3 are closely related to inflammatory process in asthma. PMID: 21575348
  61. TIM-3-TLR4 cross-regulation determined the severity of liver ischemia reperfusion injury in TLR4-dependent manner PMID: 20637206
  62. Tim3 is up-regulated in kidneys in nephrotoxic serum nephritis and Tim3 exerts protective roles in the course of disease PMID: 20167865
  63. TIM-3 is a key regulatory molecule of alloimmunity through its ability to broadly modulate CD4(+) T cell differentiation PMID: 20956339
  64. the T(H)1 cell surface molecule Tim3 has evolved to inhibit growth of intracellular pathogens via its ligand Gal9, which in turn inhibits expansion of effector T(H)1 cells to prevent further tissue inflammation. PMID: 20937702
  65. Endothelial cell-expressed Tim-3 increases melanoms cell metastatic potential by facilitating tumor cell intravasation, survival in blood stream and extravasation. PMID: 20664975
  66. Data show that targeting both PD-1 and Tim-3 is an effective immune strategy for treating chronic viral infections. PMID: 20679213
  67. The mechanisms responsible for the outcome of the Gal-9/TIM-3 interaction in normal infected mice involved direct inhibitory effects on TIM-3(+) CD8(+) T effector cells as well as the promotion of Foxp3(+) regulatory T cell activity PMID: 20463811
  68. Increased Tim-3 expression was detected in natural killer cells isolated from the liver of hepatitis B virus transgenic mice. PMID: 20133006
  69. Tim-3 expression is regulated by the Th1-specific transcription factor T-bet PMID: 20049876
  70. Mouse TIM-3 binds and phagocytoses apoptotic cells, with the BALB/c allelic variant of mouse TIM-3 showing a higher phagocytic capacity than the congenic (C.D2 Es-Hba)-allelic variant. PMID: 20083673
  71. Our data suggest that the Tim-3 pathway is highly involved in the negative regulation of inflammatory bowel disease PMID: 19913460
  72. flTim-3 but not sTim-3 may induce tumor immunological tolerance. PMID: 15989777
  73. the Tim-3-galectin-9 pathway may have evolved to ensure effective termination of effector T helper type 1 cells PMID: 16286920
  74. Soluble T cell Ig mucin 3 (Tim-3) is an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response. PMID: 16424168
  75. Tim-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response. PMID: 16709797
  76. A transient upregulation of Tim-3 expression was observed in the early stage of ACAID, suggesting its possible involvement in the development of ACAID. PMID: 16766398
  77. The interplay between multiple Tim-3-binding activities contributes to the regulated assembly of signaling complexes required for effective Th1-mediated immunity. PMID: 17363302
  78. Tim-3 inhibits TLR4 expression on mast cells and macrophages, indicating that cross- regulation occurs between these two receptors. PMID: 17513715
  79. Tim-3 function has a beneficial effect during pulmonary inflammation by skewing the Th2 response toward that of a Th1 type, suggesting an important role for Tim-3 in the regulation of allergic disease. PMID: 17517968
  80. data show that Tim-3 is highly expressed by cells of the innate immune system and that its expression on antigen-presenting cells promotes the secretion of proinflammatory cytokines from monocytes and dendritic cells PMID: 18006747
  81. Tim-3-Galectin-9 pathway plays an important role in the termination of productive Th1-immune response and could lead to developing novel therapies in transplant medicine. PMID: 18346632
  82. Gal-9 potentiates CD8(+) T cell-mediated antitumor immunity via Gal-9-Tim-3 interactions between DCs and CD8(+) T cells. PMID: 19017954
  83. a crucial role for Tim-3 in phagocytosis of apoptotic cells and cross-presentation, which may be linked to peripheral tolerance. PMID: 19224762
  84. Interaction of Tim-3 with galectin-9 plays a critical role in influencing the expression of herpes simplex virus-induced ocular lesions. PMID: 19234217
  85. Tim-3 might act as a potent regulator of antiviral T-cell responses in HBV infection. PMID: 19254478
  86. Tim-3-Galectin-9 pathway involves the function of CD4(+)CD25(+) regulatory T cells PMID: 19362679
  87. In a murine model of atopic asthma, the expression of Tim-3 is increased in CD4+ T-cells following airway challenge and likely affects asthma-induced inflammation by repressing the Th1-mediated immune response. PMID: 19905911

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Subcellular Location Isoform 1: Membrane, Single-pass type I membrane protein, Cell junction
Protein Families Immunoglobulin superfamily, TIM family
Tissue Specificity Expressed in T-helper type 1 lymphocytes. Not expressed by naive T-cells but up-regulated as they differentiate into T-helper-1 cells. Also expressed by differentiated type 1 CD8+ cytotoxic T-cells. Expressed on peritoneal exudate macrophages, monocytes,
Database Links

KEGG: mmu:171285

STRING: 10090.ENSMUSP00000020668

UniGene: Mm.72168

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