NES Monoclonal Antibody

Code CSB-MA0157131A0m
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate
    All lanes: NES antibody at 3µg/ml
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 178 kDa
    Observed band size: 260 kDa

  • Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-MA0157131A0m at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA0157131A0m at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA0157131A0m at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of PC-3 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescent analysis of U251 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Overlay histogram showing U251 cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

  • Overlay histogram showing Hela cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

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Product Details

Description

This (Nestin) NES antibody is a monoclonal antibody matched with the isotype IgG1. It is generated through sequential procedures, including the immunization of mice to get NES-producing B cells, the fusion of NES-producing B cells with myeloma cells, the screening of hybridoma cells, and the inoculation of the hybridoma cells into mice. Its immunogen is the recombinant human NES protein. The mouse ascites collected underwent protein G-mediated purification, finally harvesting the NES antibody with 95%+ purity. This NES monoclonal antibody is recommended for use in ELISA, WB, IHC, IF, and FC analyses. And it only shows reactivity with the samples containing human NES protein.

Nestin, a type VI intermediate filament, constitutes a major component of the cytoskeleton. It may participate in the modulation of assembly and disassembly of vimentin during mitosis. And it also mediates the interaction between intermediate filaments with microtubules and/or microfilaments. Importantly, nestin plays a role in essential stem cell functions, including self-renewal/proliferation, differentiation, and migration, in the context of the cytoskeleton.

Uniprot No. P48681
Alternative Names ESTM 46 antibody; FLJ 21841 antibody; FLJ21841 antibody; Intermediate filament protein antibody; Nbla00170 antibody; nes antibody; NEST_HUMAN antibody; Nestin antibody
Raised in Mouse
Species Reactivity Human
Immunogen Recombinant Human Nestin protein
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 2C2F7
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, FC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:500
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).
Gene References into Functions
  1. Nestin stabilizes lamin A-C for maintaining nuclear integrity and protecting tumor cells from senescence. PMID: 30190500
  2. We found an increased expression of Nestin and GAP43 (growth associated protein 43) in treated cells. In conclusion, Periodontal ligament mesenchymal stem cells (hPDLSCs) treated with Moringin and Cannabidiol showed an improved survival capacity and neuronal differentiation potential PMID: 30096889
  3. Melatonin disturbs SUMOylation-mediated crosstalk between c-Myc and nestin via MT1 activation and promotes the sensitivity of paclitaxel in brain cancer stem cells. PMID: 29654697
  4. Data indicated that nestin regulated NF-kappa B (NF-kappaB) activity in foetal spinal cord tissues. PMID: 29697001
  5. Nestin, originally identified as a marker of neural stem cells, was demonstrated to be expressed in breast cancer (BC). In normal breast tissue, nestin is expressed in the basal/myoepithelial cells. In BC, nestin identifies basal-like tumors and predicts aggressive behavior and poor prognosis. Also, it is been detected in BC stem cells and newly-formed tumor vessels promoting invasion and metastasis. [review] PMID: 29901100
  6. This review presents the current knowledge on the role of Nestin in essential stem cell functions, including self-renewal/proliferation, differentiation and migration, in the context of the cytoskeleton. [review] PMID: 29541793
  7. Results showed that nestin was expressed in various tissues of the embryo in patients with placenta previa, and nestin expression decreased as the embryo matured. PMID: 28833496
  8. In summary, Nestin was strongly associated with germline BRCA1 related breast cancer, a basal-like phenotype, reduced survival, and stemness characteristics. PMID: 28439082
  9. this meta-analysis indicated both CD133 and Nestin expression were significantly associated with advanced FIGO stage, larger size of residual cancer in EOC patients. PMID: 29168665
  10. Nestin expression in breast tumour cells is associated with enhanced angiogenesis and poor prognosis. PMID: 29345290
  11. results suggest that nestin might be a reliable marker for angiogenesis evaluation in patients with colorectal cancer. In contrast to CD34, the expression of nestin in blood vessels seems to be a more sensitive marker of cancer progression PMID: 29970509
  12. Benign and borderline lesions more frequently than malignant ovarian tumors displayed low or medium values of nestin-positive microvessel density. Significant correlations between PDGF-B and nestin expression in malignant tumor microvessels were also found. PMID: 28397199
  13. Nestin silencing inhibits proliferation and migration of malignant melanoma cells via G1/S arrest in a Akt-GSK3beta-Rb pathway. PMID: 29270750
  14. In this study demonstrated persistence of nestin-expressing cells in a stereotypical pattern. Striking observations included chains or rows of nestin-expressing cell in hippocampus and temporal lobe. PMID: 28925561
  15. Nestin expression was significantly higher in large cell neuroendocrine carcinomas. PMID: 29517198
  16. nestin expression in vessels was associated with a shorter overall survival (OS) and earlier relapse, and in the case of OS nestin was an independent prognostic factor. Finally, we further confirmed that nestin expression in endothelial cells reflects a progenitor nature of newly forming vessels. PMID: 28656248
  17. nestin may be a novel therapeutic target for tumor angiogenesis and a combination therapy that includes nestin-targeting agents may be an effective therapeutic approach. In addition, detection of Nestin/stem cells and Nestin/MVD can be used as predictors of disease PMID: 26945442
  18. Knockdown of Nestin significantly reduced tumor volume in vivo. PMID: 27626172
  19. Nestin plays an important role in the prediction of the clinicopathology and poor prognosis of glioma patients. PMID: 26768429
  20. The authors found that high/positive SOX2 alterations, either DNA amplification or protein expression, were favorable for overall survival (OS) in non-small cell lung cancer. On the contrary, high/positive Nestin protein expression was poor for OS. PMID: 27150062
  21. Study clarified the relationship between Nestin overexpression and poor prognosis in recurrent human hepatocellular carcinoma (HCC) patients receiving FOLFOX regimen, and its association with chemoresistance and epithelial-mesenchymal transition in HCC cell lines. PMID: 27412382
  22. Results show that the positive rates and expression levels of nestin, tyrosine hydroxylase (TH), GFAP and IL-17 were significantly decreased while Foxp3 and the ratio of Foxp3/IL-17 were statistically elevated in BM of AML patients. PMID: 27016413
  23. Nestin may have an important role in GIST malignancy by regulating mitochondrial dynamics and altering intracellular ROS levels. The findings provide new clues to reveal mechanisms by which nestin mediates the proliferation and invasion of GISTs. PMID: 26434586
  24. he present study reveals that nestin expression is a prognostic indicator of a poorer survival probability in NSCLC patients receiving adjuvant platinum-based chemotherapy , although its prognostic significance still requires confirmation with larger patient populations PMID: 28358810
  25. Data suggest that negative feedback mediated by GLI3 (GLI-Kruppel family member) acts to finely tune SHH (sonic hedgehog) signaling. During medulloblastoma (MB) formation, nerve tissue cells appear to express nestin which hyperactivates SHH signaling by abolishing negative feedback by GLI3. Restoration of intrinsic negative feedback by repressing nestin expression represents a promising approach to treat MB. [REVIEW] PMID: 28389227
  26. the induction of P-gp expression is causally associated with the expression of nestin in leukemia cells PMID: 27479651
  27. SRY (sex determining region Y)-box 10 protein (SOX10) enhances nestin protein (NES) expression via directly binding to the promoter of NES. PMID: 28189679
  28. Immunohistochemical assessment of nestin and INPP4b provides an accurate, accessible and inexpensive tool to identify basal-like breast cancer subtype in the clinically problematic setting of weak oestrogen receptor positivity PMID: 27402148
  29. Tumor expression of nestin might be a valuable prognostic factor for survival in patients with advanced ovarian cancer. With regard to its endothelial expression, nestin might be as reliable as CD34 for quantifying tumor angiogenesis. PMID: 27837619
  30. Our findings provide evidence suggesting that nestin regulation is negatively controlled epigenetically by TET2 in melanoma PMID: 27102770
  31. phosphorylation of nestin at Thr315 and/or Thr1299 affects cell proliferation, and inhibition of both phosphorylation sites suppresses invasion and metastasis of human pancreatic cancer. Inhibiting nestin phosphorylation at these two sites may represent a novel therapeutic strategy for pancreatic cancer. PMID: 28002641
  32. Nestin and CD146 are expressed in breast cancer cells with highly aggressive potency. They might contribute to disease relapse in breast cancer by activating the epithelial-mesenchymal transition pathway and assist tumor neovascularization. PMID: 28347241
  33. membrane nestin signal was found to be a valid prognostic factor in glioblastoma. PMID: 27450656
  34. OCT4 and nestin are overexpressed in laryngeal squamous cell carcinoma (LSCC) and may contribute to laryngeal carcinogenesis. Their co-expression may help to predict the lymph node metastatic potential of LSCC. PMID: 25242024
  35. The presence of nestin-positive endothelial cells is associated with early relapse of clear cell renal cell carcinoma PMID: 27311769
  36. our analysis of the expression of nestin, CD133 and ABCG2 in rhabdomyosarcomas, Ewing sarcomas and osteosarcomas represents the first complex study of these three putative CSCs markers together in three different types of pediatric sarcomas and showed their possible prognostic values in these tumors. PMID: 27314299
  37. Nestin expression seems to be obligatory for vascular smooth muscle cell proliferation, and specifies lung vascular wall cells that drive remodelling and (re-)generation PMID: 26699726
  38. Vimentin, Nestin and WT1. Sox2 was expressed by the stem/progenitor cells of the ventricular zone, whereas the postmitotic neurons of the cortical plate were immunostained by PAX2 and NSE. PMID: 26972711
  39. Administration of nestin siRNA significantly decreased primary and metastatic tumor formation by human pancreatic cancer cells. PMID: 26335012
  40. Nestin expression was consistently found in endothelial cells of the schneiderian membrane. PMID: 26414809
  41. higher rates of nestin expression were observed in most embryonal Rhabdomyosarcoma specimens and low-grade tumors, in early stages of the disease, and among younger patients. PMID: 26600525
  42. Our findings indicated that Nestin plays an essential role in colorectal cancer progression PMID: 26261513
  43. There were significant increases in the percentage of ependymal cells that were nestin positive between controls and trauma cases PMID: 25599268
  44. Nestin is expressed in acute leukemia and might be a useful immunohistochemical marker for identifying acute myeloid leukemia and acute lymphoblastic leukemia PMID: 25839093
  45. Ectopic expression of Nestin partially reverses the effects of miR-940 on cell proliferation, cell cycle and apoptosis. PMID: 25118937
  46. High level of CD133 expression trends to correlate with a worse OS and PFS in glioma patients, especially WHO IV gliomas PMID: 25967234
  47. Increased miR-21 and nestin mRNA levels were found in anaplastic meningiomas, in which recurrence is PMID: 26242334
  48. Results indicate that nestin plays an important role in the embryonic development of at least two mouse organs (heart and brain) through the regulation of cell proliferation PMID: 25843934
  49. High nestin expression is associated with gastric adenocarcinoma. PMID: 25854362
  50. It could be a clinically relevant marker associated with tumor invasiveness in cerebral astrocytomas. PMID: 25178519

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Protein Families Intermediate filament family
Tissue Specificity CNS stem cells.
Database Links

HGNC: 7756

OMIM: 600915

KEGG: hsa:10763

STRING: 9606.ENSP00000357206

UniGene: Hs.527971

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