Product Details

Uniprot No.

Alternative Names

ESTM 46 antibody; FLJ 21841 antibody; FLJ21841 antibody; Intermediate filament protein antibody; Nbla00170 antibody; nes antibody; NEST_HUMAN antibody; Nestin antibody

Raised in

Mouse

Species Reactivity

Human

Immunogen

Recombinant Human Nestin protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

2C2F7

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

This (Nestin) NES antibody is a monoclonal antibody matched with the isotype IgG1. It is generated through sequential procedures, including the immunization of mice to get NES-producing B cells, the fusion of NES-producing B cells with myeloma cells, the screening of hybridoma cells, and the inoculation of the hybridoma cells into mice. Its immunogen is the recombinant human NES protein. The mouse ascites collected underwent protein G-mediated purification, finally harvesting the NES antibody with 95%⁺ purity. This NES monoclonal antibody is recommended for use in ELISA, WB, IHC, IF, and FC analyses. And it only shows reactivity with the samples containing human NES protein.

Nestin, a type VI intermediate filament, constitutes a major component of the cytoskeleton. It may participate in the modulation of assembly and disassembly of vimentin during mitosis. And it also mediates the interaction between intermediate filaments with microtubules and/or microfilaments. Importantly, nestin plays a role in essential stem cell functions, including self-renewal/proliferation, differentiation, and migration, in the context of the cytoskeleton.

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Check Code：

Code	CSB-MA0157131A0m
Size	US$210
Image	Western Blot Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes: NES antibody at 3µg/ml Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 178 kDa Observed band size: 260 kDa Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-MA0157131A0m at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA0157131A0m at dilution of 1:100 Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA0157131A0m at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescent analysis of PC-3 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescent analysis of U251 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Overlay histogram showing U251 cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/110⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed. Overlay histogram showing Hela cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/110⁶cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NES Monoclonal Antibody

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