Code | CSB-MA0157131A0m |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:500 |
IF | 1:50-1:200 |
This (Nestin) NES antibody is a monoclonal antibody matched with the isotype IgG1. It is generated through sequential procedures, including the immunization of mice to get NES-producing B cells, the fusion of NES-producing B cells with myeloma cells, the screening of hybridoma cells, and the inoculation of the hybridoma cells into mice. Its immunogen is the recombinant human NES protein. The mouse ascites collected underwent protein G-mediated purification, finally harvesting the NES antibody with 95%+ purity. This NES monoclonal antibody is recommended for use in ELISA, WB, IHC, IF, and FC analyses. And it only shows reactivity with the samples containing human NES protein.
Nestin, a type VI intermediate filament, constitutes a major component of the cytoskeleton. It may participate in the modulation of assembly and disassembly of vimentin during mitosis. And it also mediates the interaction between intermediate filaments with microtubules and/or microfilaments. Importantly, nestin plays a role in essential stem cell functions, including self-renewal/proliferation, differentiation, and migration, in the context of the cytoskeleton.
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