Recombinant Human Nestin (NES), partial

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Code CSB-YP015713HU
MSDS
Size $250
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Neuroscience
Alternative Names
ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin
Species
Homo sapiens (Human)
Source
Yeast
Expression Region
1321-1569aa
Target Protein Sequence
PPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
27.8kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant Human NES was expressed with the amino acid range of 1321-1569. The expected molecular weight for the NES protein is calculated to be 27.8 kDa. This NES protein is produced using yeast expression system. The NES gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant NES protein during the following stages.

The human Nestin (NES), an intermediate filament protein, is a marker for neural stem and progenitor cells. NES plays a crucial role in maintaining the structural integrity of neural cells during development and in adult tissues. NES is prominently expressed in various regions of the developing nervous system, such as the neural tube, where it contributes to the formation and maintenance of neuroepithelial cells. In adult tissues, NES expression is often associated with areas of active neurogenesis and tissue repair. Beyond its role as a structural protein, NES is considered a marker for neural stem cells and is involved in regulating cell proliferation and differentiation in response to developmental signals and injury. Research on NES spans neurodevelopment, neural stem cell biology, and its potential implications in neurodegenerative diseases and regenerative medicine.

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Target Background

Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Gene References into Functions
  1. Nestin stabilizes lamin A-C for maintaining nuclear integrity and protecting tumor cells from senescence. PMID: 30190500
  2. We found an increased expression of Nestin and GAP43 (growth associated protein 43) in treated cells. In conclusion, Periodontal ligament mesenchymal stem cells (hPDLSCs) treated with Moringin and Cannabidiol showed an improved survival capacity and neuronal differentiation potential PMID: 30096889
  3. Melatonin disturbs SUMOylation-mediated crosstalk between c-Myc and nestin via MT1 activation and promotes the sensitivity of paclitaxel in brain cancer stem cells. PMID: 29654697
  4. Data indicated that nestin regulated NF-kappa B (NF-kappaB) activity in foetal spinal cord tissues. PMID: 29697001
  5. Nestin, originally identified as a marker of neural stem cells, was demonstrated to be expressed in breast cancer (BC). In normal breast tissue, nestin is expressed in the basal/myoepithelial cells. In BC, nestin identifies basal-like tumors and predicts aggressive behavior and poor prognosis. Also, it is been detected in BC stem cells and newly-formed tumor vessels promoting invasion and metastasis. [review] PMID: 29901100
  6. This review presents the current knowledge on the role of Nestin in essential stem cell functions, including self-renewal/proliferation, differentiation and migration, in the context of the cytoskeleton. [review] PMID: 29541793
  7. Results showed that nestin was expressed in various tissues of the embryo in patients with placenta previa, and nestin expression decreased as the embryo matured. PMID: 28833496
  8. In summary, Nestin was strongly associated with germline BRCA1 related breast cancer, a basal-like phenotype, reduced survival, and stemness characteristics. PMID: 28439082
  9. this meta-analysis indicated both CD133 and Nestin expression were significantly associated with advanced FIGO stage, larger size of residual cancer in EOC patients. PMID: 29168665
  10. Nestin expression in breast tumour cells is associated with enhanced angiogenesis and poor prognosis. PMID: 29345290
  11. results suggest that nestin might be a reliable marker for angiogenesis evaluation in patients with colorectal cancer. In contrast to CD34, the expression of nestin in blood vessels seems to be a more sensitive marker of cancer progression PMID: 29970509
  12. Benign and borderline lesions more frequently than malignant ovarian tumors displayed low or medium values of nestin-positive microvessel density. Significant correlations between PDGF-B and nestin expression in malignant tumor microvessels were also found. PMID: 28397199
  13. Nestin silencing inhibits proliferation and migration of malignant melanoma cells via G1/S arrest in a Akt-GSK3beta-Rb pathway. PMID: 29270750
  14. In this study demonstrated persistence of nestin-expressing cells in a stereotypical pattern. Striking observations included chains or rows of nestin-expressing cell in hippocampus and temporal lobe. PMID: 28925561
  15. Nestin expression was significantly higher in large cell neuroendocrine carcinomas. PMID: 29517198
  16. nestin expression in vessels was associated with a shorter overall survival (OS) and earlier relapse, and in the case of OS nestin was an independent prognostic factor. Finally, we further confirmed that nestin expression in endothelial cells reflects a progenitor nature of newly forming vessels. PMID: 28656248
  17. nestin may be a novel therapeutic target for tumor angiogenesis and a combination therapy that includes nestin-targeting agents may be an effective therapeutic approach. In addition, detection of Nestin/stem cells and Nestin/MVD can be used as predictors of disease PMID: 26945442
  18. Knockdown of Nestin significantly reduced tumor volume in vivo. PMID: 27626172
  19. Nestin plays an important role in the prediction of the clinicopathology and poor prognosis of glioma patients. PMID: 26768429
  20. The authors found that high/positive SOX2 alterations, either DNA amplification or protein expression, were favorable for overall survival (OS) in non-small cell lung cancer. On the contrary, high/positive Nestin protein expression was poor for OS. PMID: 27150062
  21. Study clarified the relationship between Nestin overexpression and poor prognosis in recurrent human hepatocellular carcinoma (HCC) patients receiving FOLFOX regimen, and its association with chemoresistance and epithelial-mesenchymal transition in HCC cell lines. PMID: 27412382
  22. Results show that the positive rates and expression levels of nestin, tyrosine hydroxylase (TH), GFAP and IL-17 were significantly decreased while Foxp3 and the ratio of Foxp3/IL-17 were statistically elevated in BM of AML patients. PMID: 27016413
  23. Nestin may have an important role in GIST malignancy by regulating mitochondrial dynamics and altering intracellular ROS levels. The findings provide new clues to reveal mechanisms by which nestin mediates the proliferation and invasion of GISTs. PMID: 26434586
  24. he present study reveals that nestin expression is a prognostic indicator of a poorer survival probability in NSCLC patients receiving adjuvant platinum-based chemotherapy , although its prognostic significance still requires confirmation with larger patient populations PMID: 28358810
  25. Data suggest that negative feedback mediated by GLI3 (GLI-Kruppel family member) acts to finely tune SHH (sonic hedgehog) signaling. During medulloblastoma (MB) formation, nerve tissue cells appear to express nestin which hyperactivates SHH signaling by abolishing negative feedback by GLI3. Restoration of intrinsic negative feedback by repressing nestin expression represents a promising approach to treat MB. [REVIEW] PMID: 28389227
  26. the induction of P-gp expression is causally associated with the expression of nestin in leukemia cells PMID: 27479651
  27. SRY (sex determining region Y)-box 10 protein (SOX10) enhances nestin protein (NES) expression via directly binding to the promoter of NES. PMID: 28189679
  28. Immunohistochemical assessment of nestin and INPP4b provides an accurate, accessible and inexpensive tool to identify basal-like breast cancer subtype in the clinically problematic setting of weak oestrogen receptor positivity PMID: 27402148
  29. Tumor expression of nestin might be a valuable prognostic factor for survival in patients with advanced ovarian cancer. With regard to its endothelial expression, nestin might be as reliable as CD34 for quantifying tumor angiogenesis. PMID: 27837619
  30. Our findings provide evidence suggesting that nestin regulation is negatively controlled epigenetically by TET2 in melanoma PMID: 27102770
  31. phosphorylation of nestin at Thr315 and/or Thr1299 affects cell proliferation, and inhibition of both phosphorylation sites suppresses invasion and metastasis of human pancreatic cancer. Inhibiting nestin phosphorylation at these two sites may represent a novel therapeutic strategy for pancreatic cancer. PMID: 28002641
  32. Nestin and CD146 are expressed in breast cancer cells with highly aggressive potency. They might contribute to disease relapse in breast cancer by activating the epithelial-mesenchymal transition pathway and assist tumor neovascularization. PMID: 28347241
  33. membrane nestin signal was found to be a valid prognostic factor in glioblastoma. PMID: 27450656
  34. OCT4 and nestin are overexpressed in laryngeal squamous cell carcinoma (LSCC) and may contribute to laryngeal carcinogenesis. Their co-expression may help to predict the lymph node metastatic potential of LSCC. PMID: 25242024
  35. The presence of nestin-positive endothelial cells is associated with early relapse of clear cell renal cell carcinoma PMID: 27311769
  36. our analysis of the expression of nestin, CD133 and ABCG2 in rhabdomyosarcomas, Ewing sarcomas and osteosarcomas represents the first complex study of these three putative CSCs markers together in three different types of pediatric sarcomas and showed their possible prognostic values in these tumors. PMID: 27314299
  37. Nestin expression seems to be obligatory for vascular smooth muscle cell proliferation, and specifies lung vascular wall cells that drive remodelling and (re-)generation PMID: 26699726
  38. Vimentin, Nestin and WT1. Sox2 was expressed by the stem/progenitor cells of the ventricular zone, whereas the postmitotic neurons of the cortical plate were immunostained by PAX2 and NSE. PMID: 26972711
  39. Administration of nestin siRNA significantly decreased primary and metastatic tumor formation by human pancreatic cancer cells. PMID: 26335012
  40. Nestin expression was consistently found in endothelial cells of the schneiderian membrane. PMID: 26414809
  41. higher rates of nestin expression were observed in most embryonal Rhabdomyosarcoma specimens and low-grade tumors, in early stages of the disease, and among younger patients. PMID: 26600525
  42. Our findings indicated that Nestin plays an essential role in colorectal cancer progression PMID: 26261513
  43. There were significant increases in the percentage of ependymal cells that were nestin positive between controls and trauma cases PMID: 25599268
  44. Nestin is expressed in acute leukemia and might be a useful immunohistochemical marker for identifying acute myeloid leukemia and acute lymphoblastic leukemia PMID: 25839093
  45. Ectopic expression of Nestin partially reverses the effects of miR-940 on cell proliferation, cell cycle and apoptosis. PMID: 25118937
  46. High level of CD133 expression trends to correlate with a worse OS and PFS in glioma patients, especially WHO IV gliomas PMID: 25967234
  47. Increased miR-21 and nestin mRNA levels were found in anaplastic meningiomas, in which recurrence is PMID: 26242334
  48. Results indicate that nestin plays an important role in the embryonic development of at least two mouse organs (heart and brain) through the regulation of cell proliferation PMID: 25843934
  49. High nestin expression is associated with gastric adenocarcinoma. PMID: 25854362
  50. It could be a clinically relevant marker associated with tumor invasiveness in cerebral astrocytomas. PMID: 25178519

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Protein Families
Intermediate filament family
Tissue Specificity
CNS stem cells.
Database Links

HGNC: 7756

OMIM: 600915

KEGG: hsa:10763

STRING: 9606.ENSP00000357206

UniGene: Hs.527971

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