Recombinant Bovine Serotransferrin (TF) (Active)

Serotransferrin mediates iron delivery to proliferating cells through receptor-mediated endocytosis, making functional iron-binding capacity critical for physiological relevance in cell culture models. This full-length mature protein (aa 20–704) demonstrates robust biological activity in serum-free proliferation assays, supporting MDCK cell growth with an ED50 of 0.5–2 μg/mL—a quantitative benchmark that confirms both iron-binding competence and receptor engagement. The demonstrated mitogenic activity in defined media establishes this protein as appropriate for ligand-binding and cargo-release assays investigating transferrin receptor interactions, as well as membrane transporter functional studies requiring physiologically active iron carriers. Produced in mammalian cells without affinity tags, the protein achieves >95% purity by SDS-PAGE and ≤0.5 EU/mg endotoxin, meeting the quality thresholds commonly required for antibody development, ELISA standardization, and drug-protein binding studies where native glycosylation and conformational integrity are essential.