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Recombinant Human Cadherin-17 (CDH17), partial (Active)

In Stock
Code CSB-MP613267HU
Abbreviation Recombinant Human CDH17 protein, partial (Active)
MSDS
MSDS Datasheet
Size $116
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Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human CDH17 at 2μg/mL can bind Anti-CDH17 recombinant antibody (CSB-RA613267MA1HU), the EC50 is 3.095 to 4.451 ng/mL. Biological Activity Assay
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Human CDH17 at 2 μg/mL can bind Anti-CDH17 recombinant antibody (CSB-RA613267MA1HU), the EC50 is 3.095 to 4.451 ng/mL.
Target Names
CDH17
Uniprot No.
Q12864
Alternative Names
(Intestinal peptide-associated transporter HPT-1) (Liver-intestine cadherin (LI-cadherin))
Molecular Characterization
Species
Homo sapiens (Human)
Source
Mammalian cell
Expression Region
23-787aa
Target Protein Sequence
QEGKFSGPLKPMTFSIYEGQEPSQIIFQFKANPPAVTFELTGETDNIFVIEREGLLYYNRALDRETRSTHNLQVAALDANGIIVEGPVPITIKVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLAKQSLKKQDTPQYNLTIEVSDKDFKTLCFVQINVIDINDQIPIFEKSDYGNLTLAEDTNIGSTILTIQATDADEPFTGSSKILYHIIKGDSEGRLGVDTDPHTNTGYVIIKKPLDFETAAVSNIVFKAENPEPLVFGVKYNASSFAKFTLIVTDVNEAPQFSQHVFQAKVSEDVAIGTKVGNVTAKDPEGLDISYSLRGDTRGWLKIDHVTGEIFSVAPLDREAGSPYRVQVVATEVGGSSLSSVSEFHLILMDVNDNPPRLAKDYTGLFFCHPLSAPGSLIFEATDDDQHLFRGPHFTFSLGSGSLQNDWEVSKINGTHARLSTRHTEFEEREYVVLIRINDGGRPPLEGIVSLPVTFCSCVEGSCFRPAGHQTGIPTVGM
Mol. Weight
86.3 kDa
Protein Length
Partial
Tag Info
C-terminal 10xHis-tagged
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Protein FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Cadherin-17 plays a critical role in calcium-dependent cell–cell adhesion in intestinal and hepatic epithelia, and its aberrant expression has been implicated in tumor metastasis and progression across multiple gastrointestinal cancers. This recombinant human CDH17 construct spans residues 23–787, covering the extracellular cadherin repeat domains essential for homophilic binding, and is expressed in mammalian cells with a C-terminal 10×His tag to support native-like folding and proper disulfide formation. Functional ELISA confirms robust binding activity, with an EC50 of 3.095–4.451 ng/mL against an anti-CDH17 recombinant antibody, providing a suitable basis for antibody development and validation, cell adhesion and spreading assays, and tumor metastasis research. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typically required for cell migration and invasion assays as well as integrin-ligand binding studies in sensitive cell-based systems.

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Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
4.0 - 1 reviews

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Applications : Binding assay/Protein-protein interaction

Review: I used the CSB-MP613267HU product for an ELISA experiment, and the EC50 was measured to be 1.821-4.363 ng/mL. The protein showed good binding activity, high purity, and excellent stability. I will continue to purchase in the future.

By Anonymous

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Target Background

Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.
Gene References into Functions
  1. CDH17 has a role in altering MMP-2 expression via canonical NF-kappaB signalling in human gastric cancer PMID: 29783070
  2. Data show that alteration in beta-catenin expression, a core component of the CDH17/beta-catenin axis, in tumors affects serine peptidase inhibitor Kazal type 1 (SPINK1) serum levels in hepatocellular carcinoma (HCC) patients. PMID: 28631187
  3. However, CDH17 expression was significantly elevated in patients with stage II and III gastric cancers compared to that in healthy controls (p= 0.023 and p= 0.037, respectively). PMID: 28453457
  4. CDH17 and CLDN18 are useful target molecules. Their coupling can aid in the comprehensive detection and localization of gastric cancer metastases in vivo to overcome challenges associated with intratumoral heterogeneity. PMID: 27580354
  5. CDH17 is a sensitive marker for midgut WDNETs, and the CDH17+/CDX2-/TTF1- phenotype was found to be sensitive (92%) and specific (91%) for hindgut well-differentiated neuroendocrine tumours (WDNETs). PMID: 25388236
  6. Using a lentiviral system as a delivery mediator of RNA interference, we found that inhibition of CDH17 can lead to reduce proliferation and increase apoptosis of gastric cancer cell line MKN28 in vitro and significantly diminish their tumorigenicity in vivo. Our results of the present study suggest that CDH17 may be a promising candidate for the therapeutic targeting of gastric cancer. PMID: 27909714
  7. In stomach adenocarcinomas, CDH17 positively stained 64.0% (112 of 175) of tissues, compared to CK20 and CDX2, where staining was observed in only 24.6% (43 of 175) and 46.9% (82 of 175), respectively. PMID: 28029907
  8. These data indicate that knockdown of LIcadherin facilitates the invasion of cancer cells by degrading extracellular matrix components via activation of MMP2 and 9, and increases cancer cell adhesion and migration via altered expression of galectin3. PMID: 27035870
  9. Fascin-1 expression, cadherin-17 expression, tumor size, and differentiation were independent risk factors for GC. PMID: 26743780
  10. Mutations in CDH17 gene is associated with idiopathic hypereosinophilic syndrome. PMID: 26497854
  11. Review/Meta-analysis: data reflect the role of CDH17 in tumor proliferation and metastasis among gastric cancer patients. PMID: 25834338
  12. CDH17 is a sensitive (81%) and highly specific (100%) marker for metanephric adenoma. PMID: 25768256
  13. RGD motif present in cadherin 17 induces integrin activation and tumor growth PMID: 25336636
  14. Data suggest that combined tumor expression of CDH17 (cadherin-17) and SATB2 (special AT-rich sequence binding protein 2) may be used as diagnostic biomarkers in subjects with medullary carcinoma of the large intestine (colon; cecum). PMID: 24437456
  15. Data reveal a new function for CDH17, which is to regulate alpha2beta1 integrin signaling in cell adhesion and proliferation in colon cancer cells for liver metastasis. PMID: 23604127
  16. The SNPs of the CDH17 gene c.2216 A>C might be clinically important in the prognosis of colorectal carcinoma. PMID: 23326130
  17. This study demonstrates that the secreted form of cadherin-17 (ectodomain) is truncated at the C-terminus. PMID: 23557862
  18. results identify CDH17 as a biomarker of gastric carcinoma and attractive therapeutic target for this aggressive malignancy. PMID: 23554857
  19. Cadherin-17 induces tumorigenesis and lymphatic metastasis in gastric cancer through activation of NFkappaB signaling pathway. PMID: 23298905
  20. LI-cadherin is a sensitive marker of intestinal metaplasia and can be helpful for early histologic diagnosis of Barrett's esophagus (BE); it is not, however, significantly different between BE with and without intestinal epithelial neoplasia. PMID: 23053896
  21. Up-regulation of cadherin 17 is associated with epithelial ovarian cancer progression. PMID: 22810971
  22. Report less aggressive behavior of gastric tumors after CDH17 gene knockdown. PMID: 22791949
  23. CDH17 was positively associated with larger tumor size, deeper invasion, diffuse/mixed histotype, LVI, and LNMM, predicting a poor prognosis in pN0 gastric cancer. PMID: 22009269
  24. LI cadherin is associated with an intestinal phenotype and an 'intestinal pathway' of carcinogenesis in intraductal papillary mucinous neoplasm. PMID: 22286087
  25. we proposed that CDH17 may be an oncogene up-regulating invasive features of gastric cancer cells PMID: 20393816
  26. CDH17 expression may be well preserved during the metastatic process and therefore be a useful marker for identifying colorectal adenocarcinomas in a metastatic setting with an unknown primary site PMID: 21323956
  27. Targeting CDH17 in HCC can inhibit tumor growth and inactivate Wnt signaling pathway in concomitance with activation of tumor suppressor genes.[review] PMID: 20580775
  28. CDX2 and LI-cadherin are sensitive markers of intestinal metaplasia with or without dysplasia in the upper gastrointestinal tract. PMID: 20444732
  29. identified the minimal promoter region of CDH17 that is regulated by HNF1alpha and CDX2 transcriptional factors; Suppression of HNF1alpha and CDX2 expression by siRNA down-regulated expressions of CDH17 and cyclin D1 and the viability of HCC cells PMID: 20568120
  30. Li-cadherin participates in the multiple steps of invasion and metastasis in a colorectal cancer cell line. PMID: 20204409
  31. CDH17 maybe a positive regulator for proliferative, adhesive, and invasive behaviors of gastric cancer. PMID: 20500517
  32. Expression of CDH17 and MUC13 was up-regulated in gastric cancer tissues. CDH17 is a promising prognostic marker for early stage gastric cancer. PMID: 20398667
  33. Loss of LI-cadherin results in up-regulation of MTF-1 and PlGF, thereby regulating angiogenesis in intrahepatic cholangiocarcinoma PMID: 19956853
  34. High xpression of liver-intestine cadherin and its possible interaction with galectin-3 is associated with ductal adenocarcinoma of the pancreas PMID: 12824888
  35. tumor staging and LI-cadherin expression were found to be independent factors associated with lymph node metastasis. PMID: 15178443
  36. LI-cadherin may have a role in lymph node metastasis and progression of human colorectal carcinoma PMID: 15279905
  37. Aberrant alternative splicing of LI-cadherin is associated with hepatocellular carcinoma PMID: 15701831
  38. The functional T-G haplotype of CDH17 (651 C>T and IVS6+35A>G) is a genetic susceptibility factor for the development of Hepatocellular carcinoma (HCC) in a Chinese population. PMID: 16951245
  39. Reduced expression of liver intestine-cadherin had a significant correlation with tumoral dedifferentiation and short overall survival in colorectal cancer. PMID: 17828401
  40. combined detection of CDH17/CDX2 co-expression may benefit us in predicting the prognosis of gastric carcinoma. PMID: 18353622
  41. Cadherin-17 is a useful immunohistochemical marker for diagnosis of adenocarcinomas of the digestive system. PMID: 18552820
  42. CDH17 is a novel oncogene in hepatocellular carcinoma. CDH17 is a biomarker and attractive therapeutic target for this aggressive malignancy. PMID: 19676131

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed in the gastrointestinal tract and pancreatic duct. Not detected in kidney, lung, liver, brain, adrenal gland and skin.
Database Links

HGNC: 1756

OMIM: 603017

KEGG: hsa:1015

STRING: 9606.ENSP00000027335

UniGene: Hs.591853

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