Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized CSF2RB at 2 μg/mL can bind anti-CSF2RB antibody（CSB-RA006047A0HU）, the EC₅₀ is 0.1594-0.3046 ng/mL.

Target Names

CSF2RB

Uniprot No.

P32927

Alternative Names

(CDw131)(GM-CSF/IL-3/IL-5 receptor common beta subunit)(CD antigen CD131)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

17-443aa

Target Protein Sequence

WERSLAGAEETIPLQTLRCYNDYTSHITCRWADTQDAQRLVNVTLIRRVNEDLLEPVSCDLSDDMPWSACPHPRCVPRRCVIPCQSFVVTDVDYFSFQPDRPLGTRLTVTLTQHVQPPEPRDLQISTDQDHFLLTWSVALGSPQSHWLSPGDLEFEVVYKRLQDSWEDAAILLSNTSQATLGPEHLMPSSTYVARVRTRLAPGSRLSGRPSKWSPEVCWDSQPGDEAQPQNLECFFDGAAVLSCSWEVRKEVASSVSFGLFYKPSPDAGEEECSPVLREGLGSLHTRHHCQIPVPDPATHGQYIVSVQPRRAEKHIKSSVNIQMAPPSLNVTKDGDSYSLRWETMKMRYEHIDHTFEIQYRKDTATWKDSKTETLQNAHSMALPALEPSTRYWARVRVRTSRTGYNGIWSEWSEARSWDTESVLPMW

Mol. Weight

50.9 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human CSF2RB recombinant protein was produced in mammalian cell, where the gene sequence encoding Human CSF2RB (17-443aa) was expressed with the C-terminal 6xHis tag. The purity of this CSF2RB protein was greater than 95% by SDS-PAGE. The activity has been validated.

As a β subunit shared by three cytokine receptors of IL-3, GM-CSF and IL-5, CSF2RB mainly plays the role of signaling cytokines. There are many studies on hematological diseases. CSF2RB promotes both proliferation and differentiation of hematopoietic cells, and plays an important role in maintaining the balance of proliferation, differentiation and self-renewal of hematopoietic cells. Further research on the structure and function of CSF2RB is conducive to the exploration of the malignant proliferation mechanism of leukemia cells, and lays a foundation for further research and treatment strategies for leukemia from the receptor/ligand molecular level. CSF2RB protein can be used as a specific marker protein for the diagnosis of prostate cancer bone metastasis, which makes the diagnosis of prostate cancer bone metastasis more accurate and rapid. The CSF2RB protein can also be used as a molecular drug for the preparation of prostate cancer bone metastasis, and provides a new therapeutic approach for prostate cancer bone metastasis.

Target Background

Function

High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.

Gene References into Functions

SYK mediates the actions of EPO and GM-CSF and coordinates with TGF-beta in erythropoiesis. PMID: 28131718
Exome sequencing identified candidate variants, including a missense mutation in DUOX2 that impaired its function and a frameshift mutation in CSF2RB that was associated with Crohn's Disease in an independent cohort of Ashkenazi Jewish individuals. PMID: 27373512
In a genetic analysis of Ashkenazi Jewish individuals, we associated Crohn's Disease with a frameshift mutation in CSF2RB. Intestinal monocytes from carriers of this mutation had reduced responses to granulocyte-macrophage colony-stimulating factor, providing an additional mechanism for alterations to the innate immune response in individuals with Crohn's Disease. PMID: 27377463
VitD-mediated stimulation of GC anti-inflammatory affects human monocytes in a process involving GM-CSF and MED14 PMID: 23572530
These data suggest that expression of GM-CSF and its receptor subunits by colon tumors may be a useful marker for prognosis PMID: 23108143
JAK kinase binding to betac requires the presence of three critical betac lysine residues, and this binding event is essential for receptor ubiquitination, endocytosis, and signaling. PMID: 21965659
Over expression of IL-3Ralpha and truncated mutation of hbetac may be involved in proliferation and differentiation block in NB4 cells. PMID: 21176354
Granulocyte/macrophage colony-stimulating factor causes a paradoxical increase in the BH3-only pro-apoptotic protein Bim in human neutrophils PMID: 20705940
The results of the study support CSF2RB as a risk factor common to both schizophrenia and major depression in the Chinese Han population. PMID: 21247258
Hereditary pulmonary alveolar proteinosis caused by recessive CSF2RB mutations [case report] PMID: 21205713
the up-regulation of IL-3Rbeta expression may contribute to the maintenance of proliferation rather than cell differentiation. PMID: 21207215
The beta1 integrin-interacting domain in the extracellular domain of IL-3Rbeta was identified. A fragment of a protein corresponding to domain 4 of IL-3Rbeta prevented IL-3-mediated arterial morphogenesis and endothelial cell migration. PMID: 20802515
the domain 1 D-E loop disulfide of hbetac and beta(IL-3) have roles in maintaining the precise positions of ligand-binding residues necessary for normal high affinity binding and signaling PMID: 20516062
Two different modes of beta c binding are utilized in the presence of the hIL-3R alpha isoforms. PMID: 20472554
hGM-CSF activation of hGM/beta(c)with or w/o the hbeta(c) subunit, promoted primitive phenotype maintenance, indicating that the hGM Ralpha chain cytosolic domain is needed for differentiation mediated by the hGM Ralpha, beta(c) receptor complex. PMID: 12384414
JAK2 activation mediates cross-talk between extracellular domain mutants of the beta-subunit of the GM-CSF receptor and EpoR PMID: 12488507
Interleukin-3 binding to the human betac receptor involves functional epitopes formed by domains 1 and 4 of different protein chains PMID: 15060062
A minor improvement in resolution has given a clearer indication of the position and stabilization of the key residues Tyr15, Phe79, Tyr347, His349, Ile350 and Tyr403 in the elbow region between domain 1 and domain 4 of the dimer-related molecule. PMID: 16754968
These results suggest that the JAK1-JH7-3 domains are required for interleukin-3 receptor common beta subunit interaction and abolish wild type JAK1 and JAK2-mediated signaling. PMID: 16767694
This study found that several markers (rs2072707, rs2284031 and rs909486)of csf2rb showed sex-specific and family history-dependent associations with schizophrenia. PMID: 17667962
The dynamic role of the common signaling subunit beta c is to prevent IL5alpha and GM-CSF ligand-receptor complexes from rapidly dissociating. PMID: 18294864
The interaction between intercellular adhesion molecule 1 (ICAM-1) and the GM-CSF receptor is essential for GM-CSF-induced eosinophil activation and survival. PMID: 18322230
IL-5-induced effects on betac assembly in the presence of nontagged IL-5Ralpha provide direct evidence that IL-5 can cause higher order rearrangements of betac homo-oligomers PMID: 18326494
Ligand-binding and receptor activation are not critically dependent on individual N-glycosylation sites within the IL-3- and IL-5-receptor common beta-subunit. PMID: 18374598

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Involvement in disease

Pulmonary surfactant metabolism dysfunction 5 (SMDP5)

Subcellular Location

Membrane; Single-pass type I membrane protein.

Protein Families

Type I cytokine receptor family, Type 4 subfamily

Database Links

HGNC: 2436

OMIM: 138981

KEGG: hsa:1439

STRING: 9606.ENSP00000384053

UniGene: Hs.592192

Code	CSB-MP006047HU
Abbreviation	Recombinant Human CSF2RB protein, partial (Active)
MSDS	MSDS Datasheet
Size	$116
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized CSF2RB at 2 μg/ml can bind anti-CSF2RB antibody（CSB-RA006047A0HU）, the EC₅₀ is 0.1594-0.3046 ng/mL.
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Recombinant Human Cytokine receptor common subunit beta (CSF2RB), partial (Active)

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Target Background

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