Colony-stimulating Factor 2 Receptor Beta (CSF2RB) is a transmembrane protein that constitutes the common beta chain (hβc) of cytokines IL-3, IL-5 and GM-CSF receptor. CSF2RB is required for high-affinity ligand binding and signal transduction.
CSF2RB is expressed in cells of neutrophils, eosinophils and basophils. The pleiotropic cytokines interleukins IL-3, IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) plays critical and overlapping roles in the differentiation and function of myeloid cells. They are important mediators of host defense and innate immunity, while also significantly contributing to the development and progression of inflammatory pathologies. Studies have found that CSF2RB is mainly studied in blood system diseases, which not only promotes the proliferation and differentiation of hematopoietic cells, but also plays an important role in maintaining the balance of hematopoietic cell proliferation, differentiation and self-renewal.
Recombinant Human Cytokine receptor common subunit beta(CSF2RB), partial (Active) (CSB-MP006047HU)
Validated Data
Greater than 95% as determined by SDS-PAGE.
Immobilized CSF2RB at 2 μg/ml can bind anti-CSF2RB antibody (CSB-RA006047A0HU), the EC50 is 0.1594-0.3046 ng/mL.
The following CSF2RB reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CSF2RB Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA003029 | CSF2RB Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA007339 | Phospho-CSF2RB (Y593) Antibody |
Human,Mouse,Rat | WB, IHC, ELISA |
| CSB-PA200894 | CSF2RB Antibody |
Human | ELISA,IHC |
| CSB-PA598159 | CSF2RB Antibody |
Human | ELISA,IHC |
| CSB-PA006047LA01HU | CSF2RB Antibody |
Human | ELISA, IHC, IF |
| CSB-RA006047A0HU | CSF2RB Recombinant Monoclonal Antibody |
Human | ELISA |
CSF2RB Proteins for Mus musculus (Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP006047MO CSB-EP006047MO CSB-BP006047MO CSB-MP006047MO CSB-EP006047MO-B |
Recombinant Mouse Cytokine receptor common subunit beta (Csf2rb), partial |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSF2RB Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP006047HU CSB-EP006047HU CSB-BP006047HU CSB-EP006047HU-B |
Recombinant Human Cytokine receptor common subunit beta (CSF2RB), partial |
Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
| CSB-MP006047HU | Recombinant Human Cytokine receptor common subunit beta (CSF2RB), partial (Active) |
Mammalian cell |
Colony-stimulating factor 2 receptor subunit beta (CSF2RB), a shared common beta chain of the high-affinity receptor for human granulocyte-macrophage colony-stimulating factor (GMCSF), interleukin 3 (IL-3), and interleukin 5 (IL-5) [1], is a member of the type I cytokine family of the receptors. So it is also called cytokine receptor common subunit beta. CSF2RB does not bind any cytokine by itself but converts the ligand‐bound alpha subunit (CSF2RA) to a high-affinity state for GM‐CSF, IL‐3, and IL‐5, it is therefore important for signal transduction [2][3]. CSF2RA is primarily a ligand-binding subunit with a short 54-amino acid intracellular domain that exhibits specificity for signaling. Cross‐competition of binding between these cytokines occurs by competition for the common beta subunit between different alpha subunits in the human [1]. Although CSF2RB lacks intrinsic kinase activity, the tyrosine phosphorylation of CSF2RB triggers the occurrence of multiple signaling pathways. The membrane-proximal region of CSF2RB contains a conserved proline‐rich motif termed box 1 and serves as a binding site for JAK2 [4]. Upon ligand binding and receptor oligomerization, JAK2 kinase is activated due to JAK2 transphosphorylation [4]. JAK2 phosphorylation of CSF2RB or other cytoplasmic signaling proteins is important to transmit signals from the cell surface to the nucleus. Phosphorylation of tyrosines on CSF2RB allows recruitment and subsequent activation of SH2 (src‐homology) and PTB (phosphotyrosine binding) domain proteins, ultimately resulting in the initiation of numerous downstream signaling cascades such as JAK/STAT pathway, the Ras/MAP kinase pathway, and the PI3K pathway [5][6][7]. These processes promote survival, proliferation, and differentiation. Defects in CSF2RB cause congenital pulmonary alveolar proteinosis (PAP), an autosomal recessive fatal respiratory disease.
[1] Atsushi, Miyajima Molecular structure of the IL‐3, GM‐CSF and IL‐5 receptors [J]. Volume10, Issue3, 1992, Pages 126-134.
[2] Muto A, Watanabe S, et al. High affinity chimeric human granulocyte‐macrophage colony‐stimulating factor receptor carrying the cytoplasmic domain of the beta subunit but not the alpha subunit transduces growth promoting signals in Ba/F3 cells [J]. Biochem Biophys Res Commun 1995; 208: 368-375.
[3] Bagley CJ, Woodcock JM, et al. The structural and functional basis of cytokine receptor activation: lessons from the common β subunit of the granulocyte‐macrophage colony‐stimulating factor, interleukin‐3 (IL‐3) and IL‐5 receptors [J]. Blood 1997; 89: 1471-1482.
[4] Quelle FW, Sato N, et al. JAK2 associates with the βC chain of the receptor for granulocyte‐macrophage colony‐stimulating factor, and its activation requires the membrane proximal region. Mol Cell Biol 1994; 14: 4335-4341.
[5] Mark A. Guthridge Frank C. Stomski, et al. Mechanism of Activation of the GM‐CSF, IL‐3, and IL‐5 Family of Receptors [J]. Volume16, Issue5, September 1998, Pages 301-313.
[6] Sato N, Sakamaki K, et al. Signal transduction by the high affinity GM‐CSF receptor: two distinct cytoplasmic regions of the common β subunit responsible for different signaling [J]. EMBO J 1993; 12: 4181-4189.
[7] Jucker M, Feldman RA. Identification of a new adaptor protein that may link the common beta subunit of the receptor for GM‐CSF, IL‐3 and IL‐5 to phosphatidylinositol 3‐kinase [J]. J Biol Chem 1995; 270: 27817-27822.
