Product Details

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized PROM1 at 5 μg/mL can bind Anti-PROM1 recombinant antibody (CSB-RA018751MA1HU), the EC50 is 0.4322-0.7189 ng/mL.

Target Names

PROM1

Uniprot No.

O43490

Research Area

other

Alternative Names

Prominin-1 (Antigen AC133)(Prominin-like protein 1)(CD antigen CD133)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

20-865aa

Target Protein Sequence

GGQPSSTDAPKAWNYELPATNYETQDSHKAGPIGILFELVHIFLYVVQPRDFPEDTLRKFLQKAYESKIDYDKPETVILGLKIVYYEAGIILCCVLGLLFIILMPLVGYFFCMCRCCNKCGGEMHQRQKENGPFLRKCFAISLLVICIIISIGIFYGFVANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSYWWLGGLVICSLLTLIVIFYYLGLLCGVCGYDRHATPTTRGCVSNTGGVFLMVGVGLSFLFCWILMIIVVLTFVFGANVEKLICEPYTSKELFRVLDTPYLLNEDWEYYLSGKLFNKSKMKLTFEQVYSDCKKNRGTYGTLHLQNSFNISEHLNINEHTGSISSELESLKVNLNIFLLGAAGRKNLQDFAACGIDRMNYDSYLAQTGKSPAGVNLLSFAYDLEAKANSLPPGNLRNSLKRDAQTIKTIHQQRVLPIEQSLSTLYQSVKILQRTGNGLLERVTRILASLDFAQNFITNNTSSVIIEETKKYGRTIIGYFEHYLQWIEFSISEKVASCKPVATALDTAVDVFLCSYIIDPLNLFWFGIGKATVFLLPALIFAVKLAKYYRRMDSEDVYDDVETIPMKNMENGNNGYHKDHVYGIHNPVMTSPSQH

Mol. Weight

96.7 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CD133 (Prominin-1) is a pentaspan transmembrane glycoprotein widely used as a surface marker for isolating cancer stem cells and hematopoietic progenitors, yet its five-transmembrane topology makes soluble recombinant production notoriously difficult. This construct presents the full-length mature protein (aa 20–865) in a virus-like particle (VLP) format expressed in mammalian cells, preserving native conformation and post-translational modifications critical for antibody recognition. Functional ELISA confirms robust binding activity: immobilized at 5 µg/mL, the protein binds an anti-PROM1 recombinant antibody with an EC50 of 0.43–0.72 ng/mL, supporting use as a positive control in antibody screening, specificity validation, and sandwich ELISA development. Endotoxin levels below 1.0 EU/µg, determined by LAL assay, satisfy criteria typical for cell-based binding studies and flow cytometry antibody titration experiments where lipopolysaccharide interference must be minimized.

Target Background

Function

May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.

Gene References into Functions

These findings link iNOS to Notch1 signaling in CD24(+)CD133(+) LCSCs through the activation of TACE/ADAM17. PMID: 30297396
Data show that pancreatic aldehyde dehydrogenase (ALDH(hi) cells contain a heterogeneous CD133-enriched population with a subset of beta-cell associated markers in the developing pancreas. PMID: 29285988
The downregulation of LIPC in the HepG2 cells was associated with the decreased expression of CD133, decreased cell proliferation and colony formation, as well as increased resistance to chemotherapy. PMID: 30015857
The expression of CD133 protein in patients with osteosarcoma was significantly high and related to the distant metastasis, which may be a potential prediction biomarker for poor prognosis of osteosarcoma patients PMID: 29970672
this present study shows a significant correlation between CD133 expression and differentiation of NSCLC both in our results and pooled previous results. CD133 may have prognostic significance for patients with NSCLC based on currently obtained data. PMID: 29758924
CD133 downregulation also suppressed iRFA-induced cell viability, invasion and autophagy. Collectively, our results indicated that RFA may promote residual HCC cell progression by autophagy and CD133 feedback loop. PMID: 29749472
CD133 staining was positive in 17.9% of the colorectal adenomas. Moreover, CD133 expression was associated with differentiation status (p = 0.003) and tumor size (p = 0.03). PMID: 28190751
CD133 expression and MYCN amplification induce chemoresistance and reduce average survival time in pediatric neuroblastoma. PMID: 29322842
Negative CD133 expression was an independent risk factor for a reduced disease-free survival in colon cancer with peritoneal metastasis. PMID: 29328371
this meta-analysis indicated both CD133 and Nestin expression were significantly associated with advanced FIGO stage, larger size of residual cancer in EOC patients. PMID: 29168665
Cytoplasmic and nuclear CD133 expression in colorectal adenocarcinoma cells and tumor microenvironment cells may play an important role in early colorectal adenocarcinoma carcinogenesis, while decreased CD133 nuclear expression in colorectal adenocarcinoma cells may contribute to colorectal adenocarcinoma metastasis. PMID: 30061254
CD133 expression may serve as a biomarker for early detection of gastric cancer. PMID: 30061208
Human UCB- CD133(+) hematopoietic stem cells into are remarkably potent cell candidates to transdifferentiate into motor neuron-like cells, in vitro PMID: 28754612
This study confirms that the CD133lo subset of Rb Y79 cell line is endowed with the characteristics of cancer stem cells as demonstrated by the in vitro functional assays and is in agreement with our previous findings in primary Rb tumors. PMID: 29162051
The expression of CD133 and SSEA4 determines the differentiation fate of pluripotent cancer stem cells. PMID: 28624968
CD133 is a useful predictive or prognostic biomarker for Colorectal cancer in clinical assessment and may serve as a potential therapeutic target for Colorectal cancer. PMID: 29879012
Bmi1 promoted invasion and migration of CD133+Hep G2 cells. PMID: 28849237
Lymph node metastasis is strongly associated with expression status of HGF and CD133 at the deep invasive front, suggesting the usefulness of these proteins as independent predictive markers of lymph node metastasis in early gastric cancer. PMID: 28595915
Higher hazard ratios for associations between CD133 rs2240688 polymorphism and overall survival (OS) were observed in patients with adjuvant chemotherapy and radiotherapy for curative intent. The study confirmed the significant association between the SNP rs2240688 A>C of CD133 and OS of non-small cell lung cancer patients. PMID: 28827262
glioma cells expressing CD133 are capable of modulating tumor microenvironment through the IL-1beta signaling pathway. PMID: 28736425
Exogenous CXCL3 induced Erk1/2 and ETS1 phosphorylation and promoted CD133 expression. PMID: 27255419
Low expressions of Oct4-EpCAM in IHC and CD133 in qPCR may reveal roles in gastric cancer PMID: 27557490
The clinical evidence that revealed cytoplasmic CD133 expression was correlated with poor prognosis, while nuclear CD133 expression was significantly correlated with favorable prognosis. PMID: 28687090
High CD133 expression is associated with Basal Breast Cancers. PMID: 28216417
In conclusion, p-STAT3 may participate in the progression of the early stage of colon cancer through the up-regulation of CD133, which in turn induces survivin expression. However, the regulatory mechanism of these molecules in tumor progression in vivo is need to be more verified. PMID: 29432737
Circulating tumor cells expressing cytokeratin and tumor-initiating cell markers, including ALDH, CD133, and CD44, were identified in patients with pancreatic adenocarcinoma. These TIC-like CTCs were associated with poor prognosis after surgical resection and with an increased incidence of tumor recurrence PMID: 27789528
High CD133 expression is associated with renal cell carcinoma. PMID: 27588469
CD133+CD24lo phenotype defines 5-FU-resistant human colon cancer stem cell-like cells. PMID: 27659530
Intraperitoneal enrichment of cancer stem-like cells, from ovarian cancer cell lines or primary ovarian tumor, provides a rational approach for cancer stem-like cell isolation and characterization using CD44 and prominin-1(CD133) as selection markers. PMID: 27655682
CD133 may have different roles in survival from non-small-cell lung cancer based on ethnic groups [meta-analysis] PMID: 27489355
Notch1 signaling plays an important role in the maintenance of the cancer stem-like phenotype in diffuse type gastric cancer through an RBP-Jkappa dependent pathway; inhibiting Notch1 signaling could be an effective therapy against CD133 positive diffuse type gastric cancers PMID: 27489358
Low CD133 Expression is associated with Recurrence in Colorectal Cancer with Synchronous Liver Metastasis. PMID: 26832881
CD133+ cells were genetically heterogeneous among patients without any defined profile compared to CD133-/EpCAM+ cells. PMID: 28347289
Results show that CD133 and MDR1 expression increase in recurrent pediatric pilocytic astrocytomas (PAs) following chemotherapy, and in drug resistant tumor cells. CD133 regulates MDR1 expression via the PI3K/AKT/NF-kappaB signaling. These results provide evidence that CD133 contributes to multidrug resistance by regulating MDR1 levels via the PI3K-Akt-NF-kappaB signal pathway. PMID: 28137267
CD133(+) CD44(+) CD54(+) cellular subpopulation of circulating tumor cells has a prognostic value in colorectal cancer patients with liver metastasis, especially in the survival of CRC patients with liver metastasis who did not undergo surgical treatment for metastasis. PMID: 29105339
High CD133 expression is associated with radioresistance in liver cancer. PMID: 26993601
CD133+ glioblastoma cells express molecular signatures of MSCs, neural stem cells and pluripotent stem cell PMID: 27244897
These findings indicate a novel potential anti-tumor mechanism for tubacin in CD133-expressing malignancies. PMID: 28452069
CD133 expression in colonic liver metastases is a negative prognostic factor for disease-free survival after liver resection. PMID: 29277789
The study found a significant association between the SNP rs2240688A[C of CD133 and lung cancer risk. PMID: 27130457
Results strongly indicate functional roles for CD133 in reactive oxygen species defense and in evading anticancer therapies in hepatocellular carcinoma. PMID: 28253902
High CD133 expression is associated with clear cell renal cell carcinoma. PMID: 29050853
This study described three cases in which variations in PROM1 gene may play a role in the pathogenesis of macular dystrophy or be associated with both autosomal recessive and autosomal dominant inheritance. PMID: 28095140
CD133 polymorphisms are promising biomarkers for genetic susceptibility and prognosis prediction of gastric cancer. PMID: 28326835
Low CD133 expression is associated with gastric cancer. PMID: 28218426
Our results strongly suggest epigenetic regulation of CD133 expression by promoter methylation in gastrointestinal stromal tumors PMID: 28398518
Taken together, these findings show that elevated levels of CD133 lead to Oral squamous cell carcinoma (OSCC)invasiveness and metastasis, associated with the upregulation of EMT and stemness markers. PMID: 27137188
CD133 and CD44 in glioblastoma stem cells have roles in cell proliferation, phenotype stability and intra-tumor heterogeneity PMID: 28241049
The combination of CD133 phenotyping and World Health Organization grading may assist in stratifying patients with well-differentiated pancreatic neuroendocrine tumors. PMID: 27864124
CD133 facilitates cancer stem cell-like properties by stabilizing EGFR-AKT signaling in HCC. PMID: 28034805

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Involvement in disease

Retinitis pigmentosa 41 (RP41); Cone-rod dystrophy 12 (CORD12); Stargardt disease 4 (STGD4); Retinal macular dystrophy 2 (MCDR2)

Subcellular Location

Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine.

Protein Families

Prominin family

Tissue Specificity

Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number

Database Links

HGNC: 9454

OMIM: 603786

KEGG: hsa:8842

STRING: 9606.ENSP00000415481

UniGene: Hs.614734

Code	CSB-MP018751HU(A4)
Abbreviation	Recombinant Human PROM1 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$558
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Image	CSB-MP018751HU(A4) is detected by Mouse anti-6*His monoclonal antibody. Activity Measured by its binding ability in a functional ELISA. Immobilized PROM1 at 5 μg/mL can bind Anti-PROM1 recombinant antibody (CSB-RA018751MA1HU), the EC₅₀ is 0.4322-0.7189 ng/mL.
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Recombinant Human Prominin-1 (PROM1)-VLPs (Active)

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