Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human SLC39A6 at 1 μg/mL can bind Anti- SLC39A6 recombinant antibody (CSB-RA621669MA1HU), the EC50 is 0.6873-0.9010 ng/mL.

Target Names

SLC39A6

Uniprot No.

Q13433

Alternative Names

(Estrogen-regulated protein LIV-1)(Solute carrier family 39 member 6)(Zrt- and Irt-like protein 6)(ZIP-6)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

29-325aa

Target Protein Sequence

FPQTTEKISPNWESGINVDLAISTRQYHLQQLFYRYGENNSLSVEGFRKLLQNIGIDKIKRIHIHHDHDHHSDHEHHSDHERHSDHEHHSEHEHHSDHDHHSHHNHAASGKNKRKALCPDHDSDSSGKDPRNSQGKGAHRPEHASGRRNVKDSVSASEVTSTVYNTVSEGTHFLETIETPRPGKLFPKDVSSSTPPSVTSKSRVSRLAGRKTNESVSEPRKGFMYSRNTNENPQECFNASKLLTSHGMGIQVPLNATEFNYLCPAIINQIDARSCLIHTSEKKAEIPPKTYSLQIAW

Mol. Weight

35.0 kDa

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

ZIP6 (SLC39A6) plays a critical role in cellular zinc homeostasis and has been implicated in estrogen-responsive signaling pathways, making it a key target for studying metal ion transport mechanisms and epithelial-mesenchymal transition. This recombinant human SLC39A6 partial construct, spanning residues 29–325 with a C-terminal 6xHis tag, is expressed in a baculovirus system that supports the post-translational processing important for maintaining conformational integrity of membrane transporter ectodomains. Functional ELISA validation demonstrates robust binding to an anti-SLC39A6 recombinant antibody with an EC50 of 0.69–0.90 ng/mL, confirming native-like folding and providing a suitable basis for antibody development, ELISA standardization, and ligand-binding or drug-protein interaction assays. Purity exceeding 95% by SDS-PAGE combined with endotoxin levels below 1.0 EU/μg satisfies the criteria typical for structural and biophysical characterization as well as membrane transporter functional studies.

Target Background

Function

May act as a zinc-influx transporter.

Gene References into Functions

ZIP6 deficiency disturbs intracellular Zn2+ homeostasis, leading to increased cell survival in hypoxia and reduced E-cadherin expression, indicating that decreased ZIP6 expression is strongly associated with resistance to hypoxia. PMID: 28833062
SLC39A6 promotes aggressiveness of esophageal carcinoma cells by increasing intracellular levels of zinc, activating phosphatidylinositol 3-kinase signaling, and up-regulating genes that regulate metastasis. PMID: 28209530
Upregulation of SLC39A6 is associated with hepatocellular carcinoma. PMID: 26684241
SLC39A6 may have a tumor promoting role in esophageal carcinoma PMID: 26444413
Knock-down of ZIP6 but not ZIP7 in MIN6 beta cells impaired the protective effects of GLP-1 on fatty acid-induced cell apoptosis, possibly via reduced activation of the p-ERK pathway PMID: 25969539
drug resistance of ovarian cancer cells to trichostatin A may be related to expression of the LIV1 gene PMID: 25420545
Zinc and its transporters, ZIP6 and ZIP10, are required for the breast cancer cells motility stimulated with high glucose level, such as in diabetes. PMID: 24587242
a causative role for ZIP6 in cell motility and migration PMID: 23919497
Down-regulated LIV-1 cells showed significant inhibition of proliferation in vitro and reduction of tumor growth in vivo. Furthermore, E-cadherin expression increased in LIV-1 siRNA expressing Hep-G2. PMID: 23437163
SLC39A6 has an important role in the prognosis of esophageal squamous-cell carcinoma and may be a potential therapeutic target. PMID: 23644492
Some studies correlate LIV-1 expression with a more aggressive cancer phenotype and increased likelihood for metastasis to lymph nodes. In contrast, other evidence suggests this transporter is associated with a more favorable prognosis. [review] PMID: 22852056
The results of this study showed evidence for a positive correlation between LIV1 and ZnT6 insuperior temporal, occipital, and frontal gyri in patient with alzheimer disease. PMID: 22349685
LIV-1 is involved in prostate cancer progression as an intracellular target of growth factor receptor signaling which promoted EMT and cancer metastasis PMID: 22110740
LIV-1 mRNA upregulation is associated with the progression of cervical cancer but not with the development of endometrial carcinoma. PMID: 17959546
The present study identifies LIV1 as a critical mediator responsible for HDACi-induced apoptosis. The effect of LIV1 is, at least in part, mediated by affecting intracellular zinc homeostasis. PMID: 19887557
Zip6 over-expression is not an underlying mechanism initiating breast cancer, but in fact may play a "tumor-constraining" role. PMID: 19852955
Structure-function analysis of LIV-1. PMID: 12839489
data suggest that LIV-1 protein is a promising candidate for a novel marker for breast cancer patients with better outcome PMID: 15986450
regulation of LIV-1 protein in human breast cancer xenografts PMID: 17786585
Hence, our data provide the first evidence that LIV-1 mRNA is overexpressed in cervical cancer in situ and is involved in invasion of cervical cancer cells through targeting MAPK-mediated Snail and Slug expression. PMID: 17825787
LIV-1 may be a regulator of E-cadherin PMID: 18330719
Single nucleotide polymorphism in SLC39A6 gene is associated with acute lymphoblastic leukemia. PMID: 19066393
LIV-1 enhances the aggressive phenotype through the induction of epithelial to mesenchymal transition in human pancreatic carcinoma cells. PMID: 19724917
LIV-1 co-clusters with estrogen receptor alpha in microarray analysis of breast cancer biopsies. PMID: 11911440
Review of LIV-1 and other LZT proteins. PMID: 12659941
LIV-1, a member of the ZIP family of zinc transporters, is an integral plasma membrane protein that transports zinc into cells. PMID: 12839489
LIV-1 is coregulated with estrogen receptor in some breast cancers. PMID: 12960427

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

ZIP transporter (TC 2.A.5) family

Tissue Specificity

Highly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum. Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma.

Database Links

HGNC: 18607

OMIM: 608731

KEGG: hsa:25800

STRING: 9606.ENSP00000269187

UniGene: Hs.79136

Code	CSB-BP621669HU1
Abbreviation	Recombinant Human SLC39A6 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$352
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human SLC39A6 at 1 μg/ml can bind Anti-SLC39A6 recombinant antibody (CSB-RA621669MA1HU), the EC₅₀ is 0.6873-0.9010 ng/mL.
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Recombinant Human Zinc transporter ZIP6 (SLC39A6), partial (Active)

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Target Background

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