Recombinant Human Zymogen granule protein 16 homolog B(ZG16B) (Active)

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Code CSB-MP836195HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Human ZG16B at 2 μg/ml can bind Anti-ZG16B recombinant antibody (CSB-RA836195MA1HU), the EC50 is 24.13-46.04 ng/mL. Biological Activity Assay
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Product Details

Description

This Human ZG16B recombinant protein was produced in mammalian cell, where the gene sequence encoding Human ZG16B (53-208aa) was expressed with the C-terminal 10xHis tag. The purity of this ZG16B protein was greater than 95% by SDS-PAGE. The activity was validated in functional ELISA.

ZG16B was first found to be highly expressed in pancreatic cancer and plays an important role in carcinogenesis, and was named as pancreatic cancer-related up-regulator PAUF. The latest research results show that ZG16B plays its role as an oncogene by up-regulating the expression and transcriptional activity of β-catenin in pancreatic cancer. At the same time, other studies have also found that ZG16B is not only highly expressed in pancreatic cancer, but also in colon cancer. It is also highly expressed in ovarian cancer, gastric cancer and other cancer tissues. Furthermore, ZG16B plays an important role in cancer progression such as cell proliferation, adhesion, migration, and invasion. However, in colon cancer, whether ZG16B plays a role in cancer by upregulating the expression and transcriptional activity of β-catenin and activating the Wnt/β-catenin signaling pathway is still unknown. As a novel activator of human endothelial cells, ZG16B has similar effects to vascular endothelial growth factor (VEGF), which can promote angiogenesis and increase vascular permeability in vitro and in vivo.

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/ug as determined by LAL method.
Activity Measured by its binding ability in a functional ELISA. Immobilized Human ZG16B at 2 μg/mL can bind Anti-ZG16B recombinant antibody (CSB-RA836195MA1HU), the EC50 is 24.13-46.04 ng/mL.
Target Names ZG16B
Uniprot No. Q96DA0
Molecular Characterization
Species Homo sapiens (Human)
Source Mammalian cell
Expression Region 53-208aa
Target Protein Sequence GKMYGPGGGKYFSTTEDYDHEITGLRVSVGLLLVKSVQVKLGDSWDVKLGALGGNTQEVTLQPGEYITKVFVAFQAFLRGMVMYTSKDRYFYFGKLDGQISSAYPSQEGQVLVGIYGQYQLLGIKSIGFEWNYPLEEPTTEPPVNLTYSANSPVGR
Mol. Weight 20.0 kDa
Protein Length Full Length of Mature Protein
Tag Info C-terminal 10xHis-tagged
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Gene References into Functions
  1. we conclude that the PAUF is a powerful and multifunctional promoter of tumor growth through increase and functional activation of myeloid-derived suppressor cells PMID: 27322081
  2. Mouse DCPP1 is a multifunctional promoter of tumor growth through functional activation of pancreatic cancer cells, suggesting it to be an ortholog of human PAUF. PMID: 28988106
  3. Our results suggest that PAUF has tumour-promoting functions in oral squamous cell carcinoma PMID: 27706833
  4. High PAUF expression is associated with resistance to gemcitabine in pancreatic cancer. PMID: 26684804
  5. PAUF mediated dendritic cell activation and immune stimulation are dependent on TLR4 PMID: 26336989
  6. constitutive suppression of PAUF sensitized Bxpc3 pancreatic cancer cells to oncolytic parvovirus H-1 infection PMID: 25727013
  7. PAUF rs12373A>C polymorphisms are associated with colorectal cancer. PMID: 25079514
  8. PAUF-siRNA inhibited the proliferation of colorectal cancer cells, promoted their apoptosis and induced G0/G1 cell cycle arrest. At the same time, PAUF-siRNA inhibited the invasion, adhesion and migration of the tumor cells. PMID: 23677445
  9. new possibilities for PAUF's role in the pathogenesis of angiogenesis-dependent diseases PMID: 22907431
  10. activation of SIRT1 inhibited the proliferation of pancreatic cancer -PAUF cells by down-regulation of cyclin-D1, a target molecule of beta-catenin. PMID: 22640743
  11. PAUF-mediated FAK activation plays an important role in pancreatic cancer progression. PMID: 21464589
  12. PAUF can up-regulate and stabilize beta-catenin via a novel pattern of phosphorylation, thereby contributing to the rapid proliferation of pancreatic cancer cells. PMID: 21196815
  13. Data demonstrate that the host salivary protein CSP-1 (HRPE773 GenBank AAQ89380.1) binds to S. mutans cells and may influence the initial colonization of this pathogenic bacterium onto the tooth surface. PMID: 20858015
  14. the sugar-binding site and the adjacent basic patch of ZG16p and ZG16b cooperatively form a functional glycosaminoglycan-binding site. PMID: 21110947
  15. PAUF is a mammalian lectin normally found in plant lectins. PAUF induces extracellular signal-regulated kinase phosphorylation and activates the IKK-b-mediated TPL2/MEK/ERK signaling pathway through TLR2. PMID: 20802527
  16. Findings indicate that PAUF enhances the metastatic potential of pancreatic cancer cells, at least in part, by upregulating CXCR4 expression. PMID: 19784070
  17. Bioinformatic analysis identified a putative human CSP-1/Dcpp ortholog, HRPE773, expressed predominantly in human salivary tissue, that shows 31% amino acid identity and 45% amino acid similarity to the mouse Dcpp query sequence. PMID: 16954406
  18. pancreatic adenocarcinoma up-regulated factor was secreted into the culture medium of pancreatic adenocarcinoma up-regulated factor-overexpressing Chinese hamster ovary cells, had an apparent molecular mass of approximately 25 kDa, and was N-glycosylated PMID: 19302292

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Subcellular Location Secreted.
Protein Families Jacalin lectin family
Database Links

HGNC: 30456

KEGG: hsa:124220

STRING: 9606.ENSP00000371715

UniGene: Hs.105887

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