Recombinant Macaca fascicularis natural cytotoxicity triggering receptor 3 ligand 1 (NCR3LG1), partial (Active)

NCR3LG1 serves as a ligand for the natural cytotoxicity receptor NKp30 (NCR3), modulating natural killer cell activation and cytotoxic responses in primate immune surveillance. This cynomolgus macaque ortholog, spanning residues 25–259, demonstrates robust receptor-binding activity with an EC50 of 1.171–1.377 ng/mL when paired with recombinant NCR3 in functional ELISA, providing quantitative evidence that supports its use in ligand-receptor interaction studies, competitive inhibition assays, and blocking antibody screening campaigns. Mammalian expression preserves the native glycosylation and disulfide architecture critical for conformational integrity, enabling reliable affinity characterization by surface plasmon resonance or biolayer interferometry in immune checkpoint research. The protein meets the purity and endotoxin criteria typical for therapeutic antibody epitope mapping and small-molecule inhibitor screening, with >95% purity by SDS-PAGE and <1.0 EU/μg endotoxin, aligning with standards expected in drug discovery workflows targeting NK cell-mediated immunity.