ACSL5 Antibody

Datasheet

Code	CSB-PA891734HA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Rat liver tissue All lanes: ACSL5 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 83, 74, kDa Observed band size: 76 kDa IHC image of CSB-PA891734HA01HU diluted at 1:600 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA891734HA01HU diluted at 1:600 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ACSL5 Polyclonal antibody

Uniprot No.

Q9ULC5

Target Names

ACSL5

Alternative Names

ACSL5 antibody; ACS5 antibody; FACL5 antibody; UNQ633/PRO1250 antibody; Long-chain-fatty-acid--CoA ligase 5 antibody; EC 6.2.1.3 antibody; Arachidonate--CoA ligase antibody; EC 6.2.1.15 antibody; Long-chain acyl-CoA synthetase 5 antibody; LACS 5 antibody

Raised in

Rabbit

Species Reactivity

Human, Rat

Immunogen

Recombinant Human Long-chain-fatty-acid--CoA ligase 5 protein (33-683AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ACSL5 Antibody (Product code: CSB-PA891734HA01HU) is Non-conjugated. For ACSL5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA891734HB01HU	ACSL5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA891734HC01HU	ACSL5 Antibody, FITC conjugated
Biotin	CSB-PA891734HD01HU	ACSL5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. ACSL5 may activate fatty acids from exogenous sources for the synthesis of triacylglycerol destined for intracellular storage. Utilizes a wide range of saturated fatty acids with a preference for C16-C18 unsaturated fatty acids. It was suggested that it may also stimulate fatty acid oxidation. At the villus tip of the crypt-villus axis of the small intestine may sensitize epithelial cells to apoptosis specifically triggered by the death ligand TRAIL. May have a role in the survival of glioma cells.

Gene References into Functions

our study has demonstrated that ACS5 expression was increased in colorectal cancer (CRC) cells and CRC tissues and its upregulation closely correlated to poor tumor differentiation and excess muscular layer in patients with CRC PMID: 28808653
These results suggest that ACSL1, ACSL4 and ACSL5 expression is regulated by ER signaling pathways and ACSL5 is a potential novel biomarker for predicting prognosis of breast cancer patients. PMID: 28498416
Functional variant (rs2256368:A>G) affecting ACSL5 exon 20 skipping was identified as a causal factor linked to the migraine-associated rs12355831:A>G, suggesting that the activation of long-chain fatty acids by the spliced ACSL5-Delta20 molecules is involved in migraine pathology. PMID: 27189022
colorectal adenocarcinomas with low (n=41; group 1) or high (n=31; group 2) ACSL5 levels were identified. In a one-year follow-up, tumour recurrence was significantly increased in group 1. PMID: 28153554
ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity. The molecular pathway is probably relevant for intestinal homeostasis, overwhelmed by other pathways in carcinogenesis. PMID: 25356045
Uncoupling of ACSL5 and mitochondrial mortalin by mutated TP53 could be important in colorectal carcinogenesis. PMID: 24770931
Down-regulation of ACSL5 is associated with colorectal cancer. PMID: 24222123
Levels of acyl-coenzyme A synthetase 5 in urothelial cells and corresponding neoplasias reflect cellular differentiation. PMID: 23348389
High ACSL5 transcript levels associate with systemic lupus erythematosus and apoptosis in Jurkat T lymphocytes and peripheral blood cells PMID: 22163040
The data strongly indicate that human but not rat acyl-CoA synthetase 5 is sensitive to triacsin C and does not compensate for other triacsin C-sensitive ACSL isoforms. PMID: 22171129
Increased ACSL5 is associated with Fatty Liver. PMID: 20470896
expression of ACS5 in the gastric body and the small intestine with metaplasia or heterotopia PMID: 15736044
ACS5 is a very suitable marker molecule for the detection of villus atrophy in the small intestine. PMID: 15809837
SREBP-1c-mediated insulin regulation of acyl-CoA synthestase 5 expression indicate that ACS-5 is involved in the anabolic fate of fatty acids. PMID: 16198472
Existence of a link between ACSL5 genotype and diet responsiveness. PMID: 17495181
Suggest an ACSL5-dependent regulatory mechanism that contributes to the cellular renewal along the crypt-villus axis in human small intestine. PMID: 17681178
Oncostatin M directly lowers the plasma triglycerides in hyperlipidemia by stimulating the transcription of ACSL3/5 in the liver. PMID: 17761945
Expression of ACSL5 in human epidermis is reported. PMID: 18228202
These results indicate that ACSL5 is a critical factor for survival of glioma cells under acidic tumor microenvironment. PMID: 18806831
These results reveal a mechanism for elevated transcription of ACSL5 in skeletal muscle of carriers of the rs2419621(T) allele, associated with more rapid diet-induced weight loss. PMID: 19218499

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Subcellular Location

Mitochondrion. Endoplasmic reticulum. Mitochondrion outer membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein. Cell membrane.

Protein Families

ATP-dependent AMP-binding enzyme family

Database Links

HGNC: 16526

OMIM: 605677

KEGG: hsa:51703

STRING: 9606.ENSP00000348429

UniGene: Hs.11638

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Related pathways

PPAR signaling pathway Adipocytokine signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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