AGXT2 Antibody

Datasheet

Code	CSB-PA866322LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: LO2 whole cell lysate All lanes: AGXT2 antibody at 4.9µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 50 kDa Observed band size: 50 kDa IHC image of CSB-PA866322LA01HU diluted at 1:700 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA866322LA01HU diluted at 1:700 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA866322LA01HU at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AGXT2 Polyclonal antibody

Uniprot No.

Q9BYV1

Target Names

AGXT2

Alternative Names

AGXT2 antibody; AGT2Alanine--glyoxylate aminotransferase 2 antibody; mitochondrial antibody; AGT 2 antibody; EC 2.6.1.44; antibody; R)-3-amino-2-methylpropionate--pyruvate transaminase antibody; EC 2.6.1.40 antibody; Beta-ALAAT II antibody; Beta-alanine-pyruvate aminotransferase antibody; D-AIBAT antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Alanine--glyoxylate aminotransferase 2, mitochondrial protein (196-328AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The AGXT2 Antibody (Product code: CSB-PA866322LA01HU) is Non-conjugated. For AGXT2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA866322LB01HU	AGXT2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA866322LC01HU	AGXT2 Antibody, FITC conjugated
Biotin	CSB-PA866322LD01HU	AGXT2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Can metabolize asymmetric dimethylarginine (ADMA) via transamination to alpha-keto-delta-(NN-dimethylguanidino) valeric acid (DMGV). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.

Gene References into Functions

We purified human AGXT2 from tissues of AGXT2 transgenic mice and demonstrated its ability to metabolize homoarginine to 6-guanidino-2-oxocaproic acid (GOCA). After incubation of HepG2 cells overexpressing AGXT2 with isotope-labeled homoarginine-d4 we were able to detect labeled GOCA in the medium PMID: 27752063
study in a well-characterized rheumatoid arthritis population did not show an association between serum concentrations of dimethylarginines and genetic variants of the AGXT2 gene. PMID: 28357606
AGXT2 rs37369 polymorphism is associated with increased risk for chronic heart failure, which may due to distinct disparities of alleles in asymmetric dimethylarginine degradation PMID: 27423328
Single nucleotide polymorphism in AGXT2 gene is associated with renal dysfunction in patients with chronic heart failure. PMID: 28942034
The Alanine-glyoxylate aminotransferase 2 p.V498L polymorphism is associated with both paroxysmal and chronic forms of atrial fibrillation in coronary angiographic patients without structural heart disease in ultrasound, and earlier age at onset of ischemic stroke in patients undergoing exercise stress testing. PMID: 26984639
AGXT2 rs37369 polymorphism is associated with increased risk for CHD in smokers and in diabetes mellitus patients PMID: 24834905
The AGXT2 genotype may be an important factor underlying atherosclerosis. PMID: 25620171
The results of this study that the AGXT2 gene is not associated with schizophrenia in Japanese subjects. PMID: 24727203
SNPs of AGXT2 affect plasma as well as urinary BAIB. PMID: 24586340
AGXT2 has an important role in SDMA metabolism in humans and may additionally have an unanticipated role in the autonomic nervous system regulation of cardiac function. PMID: 24159190
Alanine-glyoxylate aminotransferase-2 metabolizes endogenous methylarginines, regulates NO, and controls blood pressure. PMID: 23023372
mitochondrially localized human AGXT2 is able to effectively metabolize ADMA in vivo resulting in decreased ADMA levels and improved endothelial NO production. PMID: 20018850
Discusses the cloning of the rat gene. PMID: 7592550

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Subcellular Location

Mitochondrion.

Protein Families

Class-III pyridoxal-phosphate-dependent aminotransferase family

Database Links

HGNC: 14412

OMIM: 210100

KEGG: hsa:64902

STRING: 9606.ENSP00000231420

UniGene: Hs.34494

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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