AMOT Antibody

Datasheet
Code CSB-PA001677ESR2HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q4VCS5
Image
  • Western blot
    All lanes: Angiomotin antibody at 2μg/ml + 293T whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 119, 73 kDa
    Observed band size: 119 kDa

  • Western Blot
    Positive WB detected in: A549 whole cell lysate
    All lanes: AMOT antibody at 1.21μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 119 kDa
    Observed band size: 130 kDa

  • IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA001677ESR2HU diluted at 1:121 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen Recombinant Human Angiomotin protein (1-300AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200
Relevance Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Form Liquid
Conjugate Non-conjugated
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Angiomotin, AMOT, KIAA1071
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Research Area Cardiovascular
Target Names AMOT
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Subcellular Location Cell junction, tight junction
Protein Families Angiomotin family
Tissue Specificity Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
Database Links

HGNC: 17810

OMIM: 300410

KEGG: hsa:154796

STRING: 9606.ENSP00000361027

UniGene: Hs.528051

Pathway Hippo signaling pathway

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