APMAP Antibody

Datasheet
Code CSB-PA884629LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9HDC9
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, Rat liver tissue
    All lanes: APMAP antibody at 3.1µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 47, 33 kDa
    Observed band size: 47 kDa

  • IHC image of CSB-PA884629LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA884629LA01HU diluted at 1:400 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA884629LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Adipocyte plasma membrane-associated protein (134-240AA)
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Relevance Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Adipocyte plasma membrane-associated protein (Protein BSCv), APMAP, C20orf3
Immunogen Species Human
Research Area Signal Transduction
Target Names APMAP
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation.
Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Strictosidine synthase family
Tissue Specificity Liver, glomerular and tubular structures of the kidney, endothelial cells, arterial wall and pancreatic islets of Langerhans (at protein level). Found ubiquitously in adult as well as in embryonic tissues. In adult tissue, the highest expression is found
Database Links

HGNC: 13238

OMIM: 615884

KEGG: hsa:57136

STRING: 9606.ENSP00000217456

UniGene: Hs.472330

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