ARHGAP15 Antibody

Code CSB-PA681513LA01HU
Size US$166
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  • IHC image of CSB-PA681513LA01HU diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA681513LA01HU diluted at 1:600 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of A549 cells with CSB-PA681513LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) ARHGAP15 Polyclonal antibody
Uniprot No.
Target Names
ARHGAP15
Alternative Names
ARHGAP15 antibody; BM-024 antibody; BM-030 antibody; BM-046 antibody; Rho GTPase-activating protein 15 antibody; ArhGAP15 antibody; Rho-type GTPase-activating protein 15 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Rho GTPase-activating protein 15 protein (1-154AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The ARHGAP15 Antibody (Product code: CSB-PA681513LA01HU) is Non-conjugated. For ARHGAP15 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA681513LB01HU ARHGAP15 Antibody, HRP conjugated ELISA
FITC CSB-PA681513LC01HU ARHGAP15 Antibody, FITC conjugated
Biotin CSB-PA681513LD01HU ARHGAP15 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:500-1:1000
IF 1:200-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.
Gene References into Functions
  1. ARHGAP15 rs4662344-T ssociation with diverticular disease in Iceland and Denmark population. PMID: 28585551
  2. ARHGAP15 immunoreactivity correlated with decreased risk of recurrence and improved prognosis in breast carcinoma. ARHGA15 overexpression suppressed cell proliferation and migration of MCF-7 cells. PMID: 29534468
  3. FOXP3 can regulate the expression of ARHGAP15 and affects migration of glioma cells through the Rac1 signaling pathway PMID: 27862679
  4. ArhGAP15 plays a dual negative role in regulating small GTPase signaling, by acting at the level of the GTPase itself, as well interacting with its effector, Pak kinase. PMID: 23760270
  5. ARHGAP15 is associated with acute lung injury in mice PMID: 21297076
  6. a potential regulator of Rac1 PMID: 12650940

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
Tissue Specificity
Expressed in lung, liver and lymphoid cells.
Database Links

HGNC: 21030

OMIM: 610578

KEGG: hsa:55843

STRING: 9606.ENSP00000295095

UniGene: Hs.171011

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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