ARHGAP15 Antibody

Datasheet

Code	CSB-PA681513LA01HU
Size	US$166
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Image	IHC image of CSB-PA681513LA01HU diluted at 1:600 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA681513LA01HU diluted at 1:600 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA681513LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ARHGAP15 Polyclonal antibody

Uniprot No.

Q53QZ3

Target Names

ARHGAP15

Alternative Names

ARHGAP15 antibody; BM-024 antibody; BM-030 antibody; BM-046 antibody; Rho GTPase-activating protein 15 antibody; ArhGAP15 antibody; Rho-type GTPase-activating protein 15 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Rho GTPase-activating protein 15 protein (1-154AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ARHGAP15 Antibody (Product code: CSB-PA681513LA01HU) is Non-conjugated. For ARHGAP15 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA681513LB01HU	ARHGAP15 Antibody, HRP conjugated	ELISA
FITC	CSB-PA681513LC01HU	ARHGAP15 Antibody, FITC conjugated
Biotin	CSB-PA681513LD01HU	ARHGAP15 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.

Gene References into Functions

ARHGAP15 rs4662344-T ssociation with diverticular disease in Iceland and Denmark population. PMID: 28585551
ARHGAP15 immunoreactivity correlated with decreased risk of recurrence and improved prognosis in breast carcinoma. ARHGA15 overexpression suppressed cell proliferation and migration of MCF-7 cells. PMID: 29534468
FOXP3 can regulate the expression of ARHGAP15 and affects migration of glioma cells through the Rac1 signaling pathway PMID: 27862679
ArhGAP15 plays a dual negative role in regulating small GTPase signaling, by acting at the level of the GTPase itself, as well interacting with its effector, Pak kinase. PMID: 23760270
ARHGAP15 is associated with acute lung injury in mice PMID: 21297076
a potential regulator of Rac1 PMID: 12650940

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Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein.

Tissue Specificity

Expressed in lung, liver and lymphoid cells.

Database Links

HGNC: 21030

OMIM: 610578

KEGG: hsa:55843

STRING: 9606.ENSP00000295095

UniGene: Hs.171011

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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