Recombinant Human Rho GTPase-activating protein 15 (ARHGAP15)

Code CSB-YP681513HU
MSDS
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Source Yeast
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Code CSB-EP681513HU
MSDS
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Source E.coli
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Code CSB-EP681513HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP681513HU
MSDS
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Source Baculovirus
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Code CSB-MP681513HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARHGAP15
Uniprot No.
Alternative Names
ARHGAP15; BM-024; BM-030; BM-046; Rho GTPase-activating protein 15; ArhGAP15; Rho-type GTPase-activating protein 15
Species
Homo sapiens (Human)
Expression Region
1-475
Target Protein Sequence
MQKSTNSDTS VETLNSTRQG TGAVQMRIKN ANSHHDRLSQ SKSMILTDVG KVTEPISRHR RNHSQHILKD VIPPLEQLMV EKEGYLQKAK IADGGKKLRK NWSTSWIVLS SRRIEFYKES KQQALSNMKT GHKPESVDLC GAHIEWAKEK SSRKNVFQIT TVSGNEFLLQ SDIDFIILDW FHAIKNAIDR LPKDSSCPSR NLELFKIQRS SSTELLSHYD SDIKEQKPEH RKSLMFRLHH SASDTSDKNR VKSRLKKFIT RRPSLKTLQE KGLIKDQIFG SHLHKVCERE NSTVPWFVKQ CIEAVEKRGL DVDGIYRVSG NLATIQKLRF IVNQEEKLNL DDSQWEDIHV VTGALKMFFR ELPEPLFPYS FFEQFVEAIK KQDNNTRIEA VKSLVQKLPP PNRDTMKVLF GHLTKIVAKA SKNLMSTQSL GIVFGPTLLR AENETGNMAI HMVYQNQIAE LMLSEYSKIF GSEED
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has activity toward RAC1. Overexpression results in an increase in actin stress fibers and cell contraction.
Gene References into Functions
  1. ARHGAP15 rs4662344-T ssociation with diverticular disease in Iceland and Denmark population. PMID: 28585551
  2. ARHGAP15 immunoreactivity correlated with decreased risk of recurrence and improved prognosis in breast carcinoma. ARHGA15 overexpression suppressed cell proliferation and migration of MCF-7 cells. PMID: 29534468
  3. FOXP3 can regulate the expression of ARHGAP15 and affects migration of glioma cells through the Rac1 signaling pathway PMID: 27862679
  4. ArhGAP15 plays a dual negative role in regulating small GTPase signaling, by acting at the level of the GTPase itself, as well interacting with its effector, Pak kinase. PMID: 23760270
  5. ARHGAP15 is associated with acute lung injury in mice PMID: 21297076
  6. a potential regulator of Rac1 PMID: 12650940

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
Tissue Specificity
Expressed in lung, liver and lymphoid cells.
Database Links

HGNC: 21030

OMIM: 610578

KEGG: hsa:55843

STRING: 9606.ENSP00000295095

UniGene: Hs.171011

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