ARPC4 Antibody

Datasheet

Code	CSB-PA002130LA01HU
Size	US$166
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Image	IHC image of CSB-PA002130LA01HU diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA002130LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ARPC4 Polyclonal antibody

Uniprot No.

P59998

Target Names

ARPC4

Alternative Names

Actin related protein 2/3 complex subunit 4 antibody; Actin related protein 2/3 complex subunit 4 20 kD antibody; Actin related protein 2/3 complex subunit 4 20kDa antibody; Actin related protein 2/3 complex; 20-KD subunit antibody; Actin-related protein 2/3 complex subunit 4 antibody; ARC20 antibody; Arp2/3 complex 20 kDa subunit antibody; Arp2/3 protein complex subunit p20 antibody; ARPC 4 antibody; ARPC4 antibody; ARPC4_HUMAN antibody; MGC13544 antibody; OTTHUMP00000165252 antibody; OTTHUMP00000207520 antibody; p20 Arc antibody; p20-ARC antibody; p20ARC antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Actin-related protein 2/3 complex subunit 4 protein (2-168AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ARPC4 Antibody (Product code: CSB-PA002130LA01HU) is Non-conjugated. For ARPC4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002130LB01HU	ARPC4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA002130LC01HU	ARPC4 Antibody, FITC conjugated
Biotin	CSB-PA002130LD01HU	ARPC4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Gene References into Functions

Expression of the ARPC4 severely affects mycobacterium tuberculosis growth and suppresses immunogenic response in murine macrophages PMID: 23894563
we show cell line-specific differences in ARP2/3 complex subunit dependency on cell migratory potential, and suggest ARPC4 to be one of the key members of the ARP2/3 complex in pancreatic cancer. PMID: 23267127
We show that coronin 3 similar to other coronins interacts with the Arp2/3-complex and cofilin indicating that this family in general is involved in regulating Arp2/3-mediated events. PMID: 17274980

Subcellular Location

Cytoplasm, cytoskeleton. Cell projection. Nucleus.

Protein Families

ARPC4 family

Database Links

HGNC: 707

OMIM: 604226

KEGG: hsa:10093

STRING: 9606.ENSP00000388169

UniGene: Hs.323342

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Phage Display Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Regulation of actin cytoskeleton Fc gamma R-mediated phagocytosis Endocytosis

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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