Recombinant Human Actin-related protein 2/3 complex subunit 4(ARPC4)

Code CSB-YP002130HU
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Source Yeast
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Code CSB-EP002130HU
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Source E.coli
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Code CSB-EP002130HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002130HU
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Source Baculovirus
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Code CSB-MP002130HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ARPC4
Uniprot No. P59998
Alternative Names Actin related protein 2/3 complex subunit 4 ; Actin related protein 2/3 complex subunit 4 20 kD; Actin related protein 2/3 complex subunit 4 20kDa; Actin related protein 2/3 complex; 20-KD subunit; Actin-related protein 2/3 complex subunit 4; ARC20; Arp2/3 complex 20 kDa subunit; Arp2/3 protein complex subunit p20 ; ARPC 4; ARPC4; ARPC4_HUMAN; MGC13544; OTTHUMP00000165252 ; OTTHUMP00000207520; p20 Arc; p20-ARC; p20ARC
Species Homo sapiens (Human)
Expression Region 2-168
Target Protein Sequence TATLRPYLS AVRATLQAAL CLENFSSQVV ERHNKPEVEV RSSKELLLQP VTISRNEKEK VLIEGSINSV RVSIAVKQAD EIEKILCHKF MRFMMMRAEN FFILRRKPVE GYDISFLITN FHTEQMYKHK LVDFVIHFME EIDKEISEMK LSVNARARIV AEEFLKNF
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament.
Gene References into Functions
  1. Expression of the ARPC4 severely affects mycobacterium tuberculosis growth and suppresses immunogenic response in murine macrophages PMID: 23894563
  2. we show cell line-specific differences in ARP2/3 complex subunit dependency on cell migratory potential, and suggest ARPC4 to be one of the key members of the ARP2/3 complex in pancreatic cancer. PMID: 23267127
  3. We show that coronin 3 similar to other coronins interacts with the Arp2/3-complex and cofilin indicating that this family in general is involved in regulating Arp2/3-mediated events. PMID: 17274980
Subcellular Location Cytoplasm, cytoskeleton, Cell projection
Protein Families ARPC4 family
Database Links

HGNC: 707

OMIM: 604226

KEGG: hsa:10093

STRING: 9606.ENSP00000388169

UniGene: Hs.323342

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