ATF3 Antibody

Code CSB-PA002271EA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA002271EA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA002271EA01HU at dilution of 1:100

  • Western Blot
    Positive WB detected in: HEK293 whole cell lysate
    All lanes: ATF3 antibody at 3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 21, 14, 16, 13, 15 kDa
    Observed band size: 21 kDa

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) ATF3 Polyclonal antibody
Uniprot No. P18847
Target Names ATF3
Alternative Names Activating transcription factor 3 antibody; ATF3 antibody; ATF3_HUMAN antibody; ATF3deltaZip2 antibody; ATF3deltaZip2c antibody; ATF3deltaZip3 antibody; cAMP dependent transcription factor ATF3 antibody; cAMP-dependent transcription factor ATF-3 antibody; Cyclic AMP dependent transcription factor ATF3 antibody; Cyclic AMP-dependent transcription factor ATF-3 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Cyclic AMP-dependent transcription factor ATF-3 protein (1-181AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Carnosol suppresses IL-6 production in mouse lungs injured by ischemia-reperfusion operation and in RAW264.7 macrophages treated with lipopolysaccharide. Toru Momozane.et al,INT J BIOCHEM CELL B,2018

Sample type: RAW264.7 cells
Sample species: Mouse
Application: WB
Review: RAW264.7 cells were transformed with plasmids for RNA interference shRNA (Cont and ATF3i). After 24 h, carnosol was added to the culture medium, and ATF3 protein levels were examined by western blot analyses. The panel shows a representative set of genes analysed in duplicate. Carnosol only (third lane) resulted in the upregulation ATF3 protein.
PMID: 29958095

Target Data

Function This protein binds the cAMP response element (CRE) (consensus
Gene References into Functions
  1. Therefore, though ATF3 is activated downstream of the Wnt/beta-catenin pathway, it acts as a negative regulator of the migration and invasion of HCT116 human colon cancer cells exhibiting aberrant Wnt/beta-catenin activity. ATF3 is a candidate biomarker and target for human colorectal cancer treatment and prevention. PMID: 29966001
  2. Luciferase reporter assay identified a direct interaction of 3' UTRs of ATF3 and Runx2 with miR-590-3p in these cells. Overexpression of miR-590-3p also decreased proliferation and increased apoptosis of breast cancer cells. PMID: 30076901
  3. ATF3 mediates the inhibitory action of TNF-alpha on osteoblast differentiation and that the TNF-alpha-activated JNK pathway is responsible for the induction of Atf3 expression. PMID: 29605296
  4. these data indicated that ATF3 might promote intestinal epithelial cell apoptosis in Crohn's disease via up-regulating the stability and transcription activity of p53 PMID: 29716766
  5. electrophilic properties of itaconate and derivatives regulate the IkappaBzeta-ATF3 inflammatory axis; results demonstrate that targeting the DI-IkappaBzeta regulatory axis could be an important new strategy for the treatment of IL-17-IkappaBzeta-mediated autoimmune diseases PMID: 29670287
  6. High ATF3 expression is associated with breast cancer. PMID: 29534690
  7. ATF3 and EGR1 are involved in the beginning of inflammatory processes. Whether these two transcription factors act as tumour suppressors or promoters is context dependent and warrants analysis in further studies PMID: 28803237
  8. ATF3 and PRAP1 play important roles in cisplatin-induced DNA damage repair process. PMID: 29886035
  9. Data show that amino acid limitation (amino acid response, AAR) activation of activating transcription factor 3 (ATF3) transcription is transient relative to activation by the unfolded protein response (UPR). PMID: 29413899
  10. In patients with NAFLD and/or T2D, a significant positive correlation was observed between hepatic ATF3 expression and surrogate markers of T2D, mitochondrial dysfunction, and macrophage infiltration. PMID: 28365312
  11. our results suggest that ATF3 promotes the invasion and proliferation of CRC cells, at least in part, via the regulation of CEACAM1-mediated EMT. PMID: 28402947
  12. ATF3 is involved in promoting particulate matter-induced pulmonary inflammation. PMID: 29378244
  13. Negative pressure wound therapy exerts an anti-inflammatory effect, possibly through the suppression of proinflammatory enzymes and cytokines resulting from Ik B-alpha inhibition and ATF-3 activation, which may prevent the activation of the NF-kappaB pathway in human diabetic foot wounds. PMID: 27883358
  14. ATF3 plays a significant role in regulating human endometrial receptivity and embryo attachment in vitro via up-regulation of leukemia inhibitory factor. PMID: 28577574
  15. Upregulation of ATF3 in lung cancer promotes cell proliferation, migration, and invasion, and may represent a novel therapeutic target for lung cancer PMID: 28239957
  16. ATF3 is a negative regulator of inflammation in human fetal membranes; in primary amnion cells, ATF3 expression is induced by IL-1beta and fsl-1, and ATF3 silencing further exacerbates the inflammatory response when stimulated with these factors. Subsequently, ATF3 expression is decreased in fetal membranes after term labour and with preterm chorioamnionitis, conditions closely associated with inflammation and infection. PMID: 27780541
  17. Altering TR4-ATF3 signaling increases the efficacy of cisplatin to suppress hepatocellular carcinoma growth/progression. PMID: 27050071
  18. ATF3 may be a potential early diagnostic biomarker for silicosis and ATF3 acts as a repressor in inflammatory responses induced by silica PMID: 29102675
  19. The analysis showed higher urinary NGAL and urinary ATF3 in patients with sepsis-AKI in comparison with patients with sepsis-non-AKI and healthy volunteers. PMID: 28061889
  20. ATF3 mRNA and protein expression was significantly reduced in preterm Preeclamptic placentas. Therefore, reduced ATF3 may be centrally involved in the pathology of Preeclampsia. PMID: 28947613
  21. Data suggest that suppression of nonsense-mediated RNA decay due to persistent DNA damage (from exposure to either mutagens, gamma rays, or oxidative stress) requires the activity of p38alpha MAPK (MAPK14, mitogen-activated protein kinase 14, MAP kinase p38 alpha); mRNA of ATF3 (activating transcription factor 3) is stabilized by persistent DNA damage in a p38alpha MAPK-dependent manner. PMID: 28765281
  22. The microvascular injury phenotypes observed in vitro and in vivo were similar. ATF3 plays an important role in mediating brain microvascular responses to acute and chronic lipotoxic injury and may be an important preventative and therapeutic target for endothelial dysfunction in vascular cognitive impairment . PMID: 27087439
  23. we have identified ATF3 as an important regulator of cisplatin cytotoxicity and that ATF3 inducers in combination with platins are a potential novel therapeutic approach for NSCLC. PMID: 27659012
  24. reactivation of ATF3 is an important factor in determining sensitivity to pracinostat treatment, both in vitro and in vivo, and could serve as a potential biomarker of response and provide a rationale for therapeutic utility in HDACi-mediated treatments for bladder cancer. PMID: 27196751
  25. these findings support roles for both cFOS (indirect) and ATF3 (direct) in effecting MMP13 transcription in human chondrocytes. PMID: 27956552
  26. We functionally validated several elements for metformin-induced promoter and enhancer activity. These include an enhancer in an ataxia telangiectasia mutated (ATM) intron that has SNPs in linkage disequilibrium .Using ChIP-seq and siRNA knockdown, we further show that activating transcription factor 3 (ATF3), our top metformin upregulated AMPK-dependent gene, could have an important role in gluconeogenesis repression. PMID: 27902686
  27. ATF3 inhibition significantly increased the expression of proinflammatory cytokines interleukin 6 and interleukin 8 in cigarette smoke extract stimulated human bronchial epithelial cells. PMID: 28185985
  28. ATF3 overexpression increased PFF-A-mediated cleaved PARP. PMID: 27043582
  29. Ang II-induced upregulation of ATF3 and SUMO1 in vitro and in vivo was blocked by Ang II type I receptor antagonist olmesartan. Moreover, Ang II induced ATF3 SUMOylation at lysine 42, which is SUMO1 dependent. PMID: 26850942
  30. As a result, ATF3 rather protected the p53 wild-type cells from UV-induced apoptosis. Our results thus indicate that ATF3 regulates cell fates upon UV irradiation in a p53-dependent manner. PMID: 26994140
  31. CARMA1- and MyD88-dependent activation of Jun/ATF-type AP-1 complexes is a hallmark of ABC diffuse large B-cell lymphomas. PMID: 26747248
  32. Activating transcription factor 3 represses inflammatory responses by binding to the p65 subunit of NF-kappaB PMID: 26412238
  33. TGRL lipolysis products induce stress protein ATF3 via the TGF-beta receptor pathway, resulting in induction of apoptosis in aortic endothelial cells PMID: 26709509
  34. ATF3 regulates canonical TGFbeta and Smad signaling and fibroblast activation in systemic sclerosis. PMID: 25589515
  35. ATF3 overexpression leads to an increase of collective cell invasion phenotype in Colorectal Cancer. PMID: 26851004
  36. Data suggest that after the expression of activating transcription factor 3 (ATF3) and microRNA miR-30c-2-3p elicited by lysophosphatidic acid, miR-30c-2-3p negatively regulates the expression of ATF3 through post-transcriptional silencing. PMID: 26418018
  37. Taken together, these results suggest that ATF3 promotes the progression of human gliomas PMID: 25872784
  38. DNA damage-triggered ATF3 controlled p53 accumulation and generation of double-strand breaks may serve as a switch between DNA damage and cell death following TOP2A inhibitor treatment. PMID: 25521189
  39. The stress-responsive gene ATF3 regulates the histone acetyltransferase Tip60 stability by promoting USP7-mediated deubiquitination of Tip60. PMID: 25865756
  40. ATF3 is inactive in healthy hepatic cells and activated following hepatitis C virus infection. PMID: 25816118
  41. The function of ATF3 is to block the reactivation of virus induced by neuronal stress. PMID: 26305977
  42. It compared the induction of ATF3 protein as measured by Western blot analysis with single-molecule localization microscopy dSTORM to quantify the dynamics of accumulation of intranuclear ATF3 of triglyceride-rich (TGRL) lipolysis product-treated HAEC. PMID: 25189785
  43. SUMO modification of ATF3 influences CCND1/2 activity and cellular proliferation of prostate cancer PC3 and DU145 cells and explains at least in part how ATF3 functions to regulate cancer development. PMID: 23591848
  44. Tissue array analysis suggests role for ATF3 in regulating the occurrence of glioma and disease progression. PMID: 26051724
  45. Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element. PMID: 25931127
  46. these results suggest that there is a negative feedback expression of pre-mir-590 and its putative target gene, ATF-3 in human breast cancer cells. PMID: 25150595
  47. ATF3 is a downstream target of SRC and CaMKII signaling, and may be involved in adrenocortical aldosterone synthesis. PMID: 25400120
  48. Urotensin-II induces activating transcription factor 3 (ATF3) at both mRNA and protein levels. PMID: 25268131
  49. Suggest critical role for tumor suppressor ATF3 in esophageal squamous cell carcinoma progression. PMID: 25149542
  50. ATF-3 was associated with Runx2 promoter in MDA-MB231 cells. PMID: 25380580
  51. ATF3 is lower in hepatocellular carcinoma than in hepatic hemangioma. The protein level is lower in patients with capsule invasion, while there is no association with other main clinical pathological features PMID: 24906227
  52. ATF3 is not highly associated with hypospadias in metropolitan Tokyo. Differences in ethnicity might have influenced our results. PMID: 24469490
  53. The gene expression profiling results showed that TNFAIP3, ATF3, PPARG are related to osteoarthritis. PMID: 23884832
  54. in human HCC tissues, SPTBN1 expression correlated negatively with expression levels of STAT3, ATF3, and CREB2; SMAD3 expression correlated negatively with STAT3 expression PMID: 25096061
  55. inhibition of cell viability and apoptosis by PCA may be result of ATF3 expression through ERK1/2 and p38-mediated transcriptional activation. PMID: 25447816
  56. Counterbalance between MIC-1 and ATF3 is critical for deciding the fate of enterocytes under the food chemical stress. PMID: 25180886
  57. show that ATF3 promotes colon cancer metastasis PMID: 24859834
  58. results provide novel insights into the role of ATF3 as an essential transcription factor for p53-independent DR5 induction upon both ZER and CCB treatment, and this may be a useful biomarker for TRAIL-based anticancer therapy PMID: 24939851
  59. superinduced ATF3 attenuated ER stress-induced cancer chemokine expression by epigenetically interfering with induction of EGR-1, a transcriptional modulator crucial to cancer chemokine production. PMID: 25122760
  60. Atf3-silencing reversed ethanol-mediated Gck down-regulation and beta-cell dysfunction, followed by the amelioration of impaired glucose tolerance and insulin resistance. PMID: 25074928
  61. High ATF3 activates Stat3 phosphorylation through inhibition of p53 expression in skin cancer PMID: 24460316
  62. We analyzed the influence of UV radiation and cyclosporine A on ATF3 expression and its potential role in squamous cell carcinoma development. PMID: 24509533
  63. Epithelium-derived exosomal ATF3 RNA attenuates ischemia-reperfusion induced kidney injury by inhibiting MCP-1 gene transcription. PMID: 24420912
  64. ATF-3 is involved in the progress of laryngeal squamous cell carcinoma, and may provide clinical information for evaluation of its prognosis. The oncologic role of ATF-3 may be correlated with Cyclin D1 regulation. PMID: 24133584
  65. Data indicate that pharmacological or genetic inactivation of PIKfyve rapidly induces expression of the transcription repressor ATF3, which is necessary and sufficient for suppression of type I IFN expression. PMID: 24600036
  66. ATF3 can suppress mutp53 oncogenic function, thereby contributing to tumor suppression in TP53-mutated cancer PMID: 24554706
  67. These findings strongly suggest that ATF3 is involved in suppressing IL-6 cytokine expression during gonococcal infection. PMID: 23648135
  68. In hippocampal tissue from patients with pharmacoresistant medial temporal lobe epilepsy, we observed an inverse correlation of mrna expression to seizure frequency per month. PMID: 23706953
  69. Transcription factor ATF-3 regulates allele variation phenotypes of the human SLC11A1 gene. PMID: 23187734
  70. protects against hemin cytotoxicity PMID: 23560949
  71. These findings demonstrate that ribosomal stress-induced ATF3 is a critical regulator in the convergent pathways between EGR-1 and NF-kappaB, which contributes to the suppression of Nod2-activated proinflammatory gene expression. PMID: 24098051
  72. Triglyceride-rich lipoprotein lipolysis products activate ATF3-JNK transcription factor networks and induce endothelial cells inflammatory response. PMID: 23868936
  73. ATF3 is a regulator in myeloid cells that enhances breast cancer metastasis and has predictive value for clinical outcomes. PMID: 23921126
  74. In CSB-deficient cells ATF3 remains bound to the promoter, thereby preventing the arrival of polymerase II and the restart of transcription. PMID: 23733932
  75. Data indicate that activating transcription factor 3 (ATF3) upregulated actin filament severing protein gelsolin (GSN), whereas ATF3 silencing reduced GSN levels, concomitant with alterations in the actin cytoskeleton. PMID: 23536558
  76. Results indicate that both increased cJUN and the cis-acting ATF/CRE sequence within the ATF3 promoter contribute to the transcriptional activation of the gene during the amino acid response (AAR). PMID: 23269699
  77. ATF3 is post-translationally modified by SUMO and SUMOylation of ATF3 plays a functional role in regulation of TP53 gene activity. PMID: 22991139
  78. the recruitment of multiple HDAC members to JDP2 and ATF3 is part of their transcription repression mechanism. PMID: 22989952
  79. The amino acid response that results in transcriptional activation increases transcription of AFT3 and asparagine synthetase. PMID: 22978410
  80. Interaction between genetic and environmnetal factors may explain nonreplication in genetic studies of hypospadias. PMID: 23088992
  81. Salermide induces expression of ATF4, and ATF4 up-regulates ATF3 and subsequently modulates CHOP which leads to apoptosis in human cancer cells. PMID: 21801305
  82. ATF3 expression is a surrogate of malignancy (or pre-malignancy) in keratinocytic epith-elial proliferations and thus helps distinguish squamous cell carcinoma from other cutaneous epithelial neoplasms. PMID: 22764884
  83. Human ATF3 normalizes fat content and gene expression in atf3 Drosophila melanogaster mutants. PMID: 22851689
  84. the role of cholesterol in prostate health, and provide a novel role for ATF3, and associated proteins within a large signaling network, as a cholesterol-sensing mechanism. PMID: 22768301
  85. ATF3 protein expression was up-regulated after cytotoxic doses of cisplatin treatment and it directly bound to the CHOP gene promoter, increasing this pro-apoptotic protein's expression. PMID: 22753726
  86. statistical association between variations in the ATF3 gene and levels of nine serum inflammatory markers, including C reactive protein PMID: 21324310
  87. Opposite changes in the expression of ATF-3 and ATF-4 genes in atherosclerotic and psoriatic samples were revealed and a hypothesis was put forward that this parameter could be a criterion of pathological process in both diseases. PMID: 22485215
  88. our results provide a novel insight into the role of ATF3 as an essential co-transcription factor for p53 upon DNA damage PMID: 21927023
  89. these results demonstrate that ATF3 represses the C/EBPalpha gene, resulting in inhibition of adipocyte differentiation, and thus plays a role in hypoxia-mediated inhibition of adipocyte differentiation. PMID: 22475484
  90. an extensive network of stress-inducible transcription factors and demonstrate that ATF3 has opposing, cell context-dependent effects on p53 target genes in DNA damage response and cancer development PMID: 22046379
  91. the loss of the major reactive oxygen species scavenger, glutathione, during SAIS directly correlates with an increase in the expression of activating transcription factor 3. PMID: 22179317
  92. found that the ability of stromal ATF3 to promote cancer progression is mediated by transcriptional repression of CLDN1 and induction of CXCL12 and RGS4 PMID: 21900211
  93. ATF3 bound to the TREM-1 promoter. PMID: 21421043
  94. these results demonstrate that ATF3 represses PDX-1 expression via binding to an ATF3-responsive element in its promoter, which plays an important role in suppression of pancreatic beta-cells function. PMID: 21821004
  95. activating transcription factor 3 appears to play an oncogenic role in the development of squamous cell carcinomas and may be related to the biologic behavior of them. PMID: 21315420
  96. KAI1 gene is engaged in NDRG1 gene-mediated metastasis suppression through the ATF3-NFkappaB complex in human prostate cancer. PMID: 21454613
  97. MUC18 is involved in cell signaling regulating the expression of Id-1 and ATF-3, thus contributing to melanoma metastasis PMID: 21467165
  98. ATF3 interacts with HTLV-I bZIP factor and promotes proliferation of ATL cells. PMID: 21414204
  99. LDLR repression by organelle stress via ATF3 PMID: 21294679
  100. Traumatic axonal injury in transgenic mice induces an early and sustained activation of c-Jun and ATF-3 (activating transcription factor 3), known regulators of axon regeneration. PMID: 21451046

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Subcellular Location Nucleus
Protein Families BZIP family, ATF subfamily
Database Links

HGNC: 785

OMIM: 603148

KEGG: hsa:467

STRING: 9606.ENSP00000344352

UniGene: Hs.460

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