Acetyl-H2AFZ (K11) Antibody

Datasheet

Code CSB-PA010100PA11acHU
Size US$319
Uniprot No. P0C0S5
Abbreviation Histone H2A.Z
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) tissue using CSB-PA010100PA11acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8μg anti-H2AFZ (CSB-PA010100PA11acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate ( treated with 30mM sodium butyrate for 4h)
    All lanes: H2AFZ antibody at 1μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 14 kDa
    Observed band size: 14 kDa

Immunogen Peptide sequence around site of acetyl-Lys (11) derived from human Histone H2A.Z.
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF, ChIP; Recommended dilution: WB:1:200-1:2000, IF:1:50-1:200
Relevance Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H2A.Z (H2A/z), H2AFZ, H2AZ
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
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Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2A family
Database Links

HGNC: 4741

OMIM: 142763

KEGG: hsa:3015

STRING: 9606.ENSP00000296417

UniGene: Hs.119192

Pathway Necroptosis

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