Acetyl-H2AFZ (K4) Antibody

Datasheet
Code CSB-PA010100OA04acHU
Size US$319
Uniprot No. P0C0S5
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate (treated by 30mM sodium butyrate for 4h)
    All lanes: H2AFZ antibody at 1.06µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 14 kDa
    Observed band size: 14 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA010100OA04acHU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (CSB-PA010100OA04acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • Immunocytochemistry analysis of CSB-PA010100OA04acHU diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating H2AFZ in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)
    Lane 1: Rabbit control IgG instead of CSB-PA010100OA04acHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA010100OA04acHU (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg)
    Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Immunogen Peptide sequence around site of Acetyl-Lys (4) derived from Human Histone H2A.Z
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, ICC, IF, IP, ChIP; Recommended dilution: WB:1:200-1:2000, ICC:1:10-1:100, IF:1:10-1:100, IP:1:200-1:2000
Relevance Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Histone H2A.Z (H2A/z), H2AFZ, H2AZ
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names H2AFZ
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2A family
Database Links

HGNC: 4741

OMIM: 142763

KEGG: hsa:3015

STRING: 9606.ENSP00000296417

UniGene: Hs.119192

Pathway Necroptosis

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