Acetyl-HIST1H2AG (K36) Antibody

Code CSB-PA010389PA36acHU
Size US$319
Image
  • Immunocytochemistry analysis of Hela cells using CSB-PA010389PA36acHU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010389PA36acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CSB-PA010389PA36acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate (All treated with 30mM sodium butyrate for 4h)
    All lanes: HIST1H2AG antibody at 1µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 15 kDa
    Observed band size: 15 kDa

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) HIST1H2AG Polyclonal antibody
Uniprot No. P0C0S8
Target Names HIST1H2AG
Alternative Names H2AC11 antibody; H2AFP antibody; HIST1H2AG; antibody; H2AC13 antibody; H2AFC antibody; HIST1H2AI; antibody; H2AC15 antibody; H2AFD antibody; HIST1H2AK; antibody; H2AC16 antibody; H2AFI antibody; HIST1H2AL; antibody; H2AC17 antibody; H2AFN antibody; HIST1H2AMHistone H2A type 1 antibody; H2A.1 antibody; Histone H2A/ptl antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Acetyl-Lys (36) derived from Human Histone H2A type 1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, ICC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
WB 1:200-1:2000
ICC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2A family
Database Links

HGNC: 4737

OMIM: 602787

KEGG: hsa:8329

STRING: 9606.ENSP00000352627

UniGene: Hs.134999

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