Acetyl-HIST1H2BB (K16) Antibody

Datasheet
Code CSB-PA010402OA16acHU
Size US$319
Image
  • Immunocytochemistry analysis of Hela cells using CSB-PA010402OA16acHU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells treated with NaB using CSB-PA010402OA16acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2BB (CSB-PA010402OA16acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Product Details

Full Product Name Rabbit anti-Human HIST1H2BB Polyclonal antibody
Uniprot No. P33778
Target Names HIST1H2BB
Alternative Names Histone H2B type 1-B (Histone H2B.1) (Histone H2B.f) (H2B/f), HIST1H2BB, H2BFF
Raised in Rabbit
Species Reactivity Human
Immunogen Peptide sequence around site of Acetyl-Lys (16) derived from Human Histone H2B type 1-B
Immunogen Species Human
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen Affinity Purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, ICC, IF, ChIP
Recommended Dilution
Application Recommended Dilution
ICC 1:20-1:200
IF 1:50-1:200
Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location Nucleus, Chromosome
Protein Families Histone H2B family
Database Links

HGNC: 4751

OMIM: 602803

KEGG: hsa:3018

STRING: 9606.ENSP00000350580

UniGene: Hs.553494

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