HIST1H2BB Antibody

Datasheet

Code	CSB-PA010402HA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse liver tissue, Rat liver tissue All lanes: HIST1H2BB antibody at 2.8μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA010402HA01HU diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of PC-3 cells using CSB-PA010402HA01HU at dilution of 1:266 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HIST1H2BB Polyclonal antibody

Uniprot No.

P33778

Target Names

HIST1H2BB

Alternative Names

HIST1H2BB antibody; H2BFFHistone H2B type 1-B antibody; Histone H2B.1 antibody; Histone H2B.f antibody; H2B/f antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Histone H2B type 1-B protein (2-126AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HIST1H2BB Antibody (Product code: CSB-PA010402HA01HU) is Non-conjugated. For HIST1H2BB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA010402HB01HU	HIST1H2BB Antibody, HRP conjugated	ELISA
FITC	CSB-PA010402HC01HU	HIST1H2BB Antibody, FITC conjugated
Biotin	CSB-PA010402HD01HU	HIST1H2BB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene References into Functions

Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants has been uncovered. PMID: 29643390
RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID: 26854224
RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID: 21362548

Subcellular Location

Nucleus. Chromosome.

Protein Families

Histone H2B family

Database Links

HGNC: 4751

OMIM: 602803

KEGG: hsa:3018

STRING: 9606.ENSP00000350580

UniGene: Hs.553494

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

What Are Histones: Unraveling Their Critical Roles in Disease Pathogenesis Unraveling the Dynamic World of Histone Modifications: Implications in Gene Regulation and Disease Pathogenesis The Overview of Organelles Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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