BRAP Antibody

Code CSB-PA773793LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: Mouse Brain tissue lysate, Mouse Heart tissue lysate, Rat Heart tissue lysate,
    All lanes: BRAP antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 68 kDa
    Observed band size: 68 kDa
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate
    All lanes: BRAP antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 68 kDa
    Observed band size: 68 kDa
  • IHC image of CSB-PA773793LA01HU diluted at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
  • Immunofluorescence staining of Hela cell with CSB-PA773793LA01HU at 1:130, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) BRAP Polyclonal antibody
Uniprot No.
Target Names
BRAP
Alternative Names
BRAP antibody; RNF52BRCA1-associated protein antibody; EC 2.3.2.27 antibody; BRAP2 antibody; Impedes mitogenic signal propagation antibody; IMP antibody; RING finger protein 52 antibody; RING-type E3 ubiquitin transferase BRAP2 antibody; Renal carcinoma antigen NY-REN-63 antibody
Raised in
Rabbit
Species Reactivity
Human, Rat, Mouse
Immunogen
Recombinant Human BRCA1-associated protein (13-124AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The BRAP Antibody (Product code: CSB-PA773793LA01HU) is Non-conjugated. For BRAP Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA773793LB01HU BRAP Antibody, HRP conjugated ELISA
FITC CSB-PA773793LC01HU BRAP Antibody, FITC conjugated
Biotin CSB-PA773793LD01HU BRAP Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
Gene References into Functions
  1. Six polymorphisms (rs12229654 at 12q24.1, rs671 of ALDH2, rs11066015 of ACAD10, rs2074356 and rs11066280 of HECTD4, and rs3782886 of BRAP) were found to be associated with both systolic and diastolic blood pressure, with those at 12q24.1 or in ACAD10 or BRAP being novel determinants of blood pressure in Japanese. PMID: 28562329
  2. IMPACT data were consistent with increased risks of onset among BRCA1 and BRCA2 mutation carriers PMID: 27742670
  3. Expression of BRAP is increased in esophageal squamous cell carcinoma samples compared with non-tumor esophageal tissues; increased expression correlates with reduced patient survival time and promotes metastasis of xenograft tumors in mice. PMID: 28780075
  4. Together the results indicate for the first time that BRAP2 may play an important NRNI role in germ cells of the testis, with an additional, scaffold/structural role in mature spermatozoa. PMID: 25820252
  5. BRAP gene may confer vulnerability for schizophrenia in Han Chinese population. PMID: 24454952
  6. ectopic expression of BRAP2 inhibits nuclear localization of HMG20A and NuMA1, and prevents nuclear envelope accumulation of SYNE2. PMID: 23707952
  7. genetic association studies in population of Chinese Han young adults in Beijing: Data suggest that 2 SNPs in BRAP (rs11066001; rs3782886) are associated with decreased risk of metabolic syndrome in this population. PMID: 22965072
  8. BAP1 expression closely correlates with age, clinical stage, pathologic differentiation and histological type in colorectal cancer (CRC). BAP1 may serve as a novel prognostic biomarker for CRC. PMID: 23526420
  9. Brap2 regulates temporal control of NF-kappaB localization mediated by inflammatory response. PMID: 23554956
  10. The BRAP polymorphism may not play an important role in ischemic stroke in the Taiwanese population. PMID: 23356535
  11. The dominant effect of prolonged USP15 depletion upon signal amplitude is due to a decrease in CRAF levels while allowing for the possibility that USP15 may also function to dampen MAPK signaling through direct stabilization of BRAP. PMID: 23105109
  12. ANRIL on 9p21 and BRAP were both associated with ankle brachial index in a Taiwanese population. PMID: 22122968
  13. Several BRCA1 mutations were observed in Pakistani breast cancer patients with moderate family history. PMID: 22078348
  14. Data show that BRAP conferred a risk for carotid plaque and intima-medial thickness. PMID: 21670849
  15. BRAP gene is associated with the extent of coronary atherosclerosis and has a synergistic effect with diabetes on the occurrence of significant CAD in the Chinese population PMID: 22085839
  16. our approach, which is applicable to any set of interval scale traits that is heritable and exhibits evidence of phenotypic clustering, identified three new loci in or near APOC1, BRAP, and PLCG1, which were associated with multiple phenotype domains. PMID: 22022282
  17. Polymorphism of 270 A > G in BRAP is Associated with Lower Ankle-Brachial Index and peripheral artery disease. PMID: 21301165
  18. All results are consistent with BRAP2 being a novel, phosphorylation-regulated negative regulator of nuclear import, with potential as an antiviral agent. PMID: 20040518
  19. Data indicate that one SNP in BRAP showed(rs11066001) a significant association in allele frequency distribution with CAD in both the Japanese and Korean populations. PMID: 19713974
  20. modulates sensitivity of the MAP kinase cascade to stimulus-dependent activation by limiting functional assembly of the core enzymatic components through the inactivation of KSR PMID: 14724641
  21. Monocytic differentiation of the promyelomonocytic cell lines U937 and HL60 is associated with the upregulation of Brap2 expression concomitantly with the upregulation and cytoplasmic relocalization of p21. PMID: 15340083
  22. the capacity of IMP to inhibit signal propagation through Raf to MEK is a consequence of disrupting KSR1 homooligomerization and B-Raf/c-Raf hetero-oligomerization PMID: 18332145
  23. The most current evidence suggests that it may be more beneficial in those with BRCA mutations because tumors associated with these mutations are likely to be estrogen-receptor positive. PMID: 18380995
  24. p21 Ras/Imp regulate cytokine production and migration in CD4 T cells [Imp] PMID: 18577512
  25. Carriers of mutations in the genes BRCA1/2 may present a specific high risk group for PABC especially at younger ages. PMID: 19009954
  26. The authors found that Brap2, which has intrinsic RING domain dependent E3 ligase activity, facilitates HsCdc14A Lys-63 linked ubiquitin modification, indicating that Brap2 may be the ubiquitin E3 Ligase of HsCdc14A. PMID: 19152073
  27. SNPs in BRAP associated with risk of myocardial infarction in Asian populations.BRAP knockdown in cultured coronary endothelial cells suppressed activation of NF-kappaB. PMID: 19198608

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Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed in breast epithelial cell lines.
Database Links

HGNC: 1099

OMIM: 604986

KEGG: hsa:8315

STRING: 9606.ENSP00000403524

UniGene: Hs.530940

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