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Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010429OA16butHU, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Positive WB detected in:K562 whole cell lysate, HepG2 whole cell lysate(treated by 30mM sodium butyrate for 4h)
All lanes:HIST1H4A antibody at 1.6ug/ml
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Peptide sequence around site of butyrly-Lys(16) derived from human Histone H4
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.