Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate(treated by 30mM sodium butyrate for 4h)
All lanes: HIST1H4A antibody at 1.9ug/ml
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010429OA08butHU at 1:37.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunocytochemistry analysis of CSB-PA010429OA08butHU diluted at 1:75 and staining in Hela cells(treated with 30mM sodium butyrate for 4h)performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Peptide sequence around site of butyrly-Lys(8) derived from human Histone H4
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.