CEP135 Antibody

Code CSB-PA721063LA01HU
Size US$166
Order now
Image
  • Immunofluorescence staining of A549 cells with CSB-PA721063LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CEP135 Polyclonal antibody
Uniprot No.
Target Names
CEP135
Alternative Names
centrosomal protein 135 kDa antibody; centrosomal protein 135kDa antibody; Centrosomal protein 4 antibody; Centrosomal protein of 135 kDa antibody; centrosome protein 4 antibody; centrosome protein cep135 antibody; Cep135 antibody; CEP4 antibody; CP135_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Centrosomal protein of 135 kDa protein (94-195AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CEP135 Antibody (Product code: CSB-PA721063LA01HU) is Non-conjugated. For CEP135 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA721063LB01HU CEP135 Antibody, HRP conjugated ELISA
FITC CSB-PA721063LC01HU CEP135 Antibody, FITC conjugated
Biotin CSB-PA721063LD01HU CEP135 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner.
Gene References into Functions
  1. CEP135 segment 96-108 is a major microtubule-binding site. PMID: 27477386
  2. identified the second mutation in CEP135, confirming the role during embryonic brain development and in the pathophysiology of human primary microcephaly PMID: 26657937
  3. Authors suggest that the decrease in CEP135(mini) from centrosomes promotes centriole assembly. The repression of centriole duplication by a splice isoform of a protein that normally promotes it serves as a novel mechanism to limit centriole duplication. PMID: 26412126
  4. These findings support a model in which PKC-mediated phosphorylation of CEP4 at Ser(18) and Ser(80) causes its dissociation from Cdc42, thereby increasing its affinity for TEM4 and producing Rac activation, filopodium formation, and cell motility PMID: 25086031
  5. Authors propose that CEP135 directly connects the central hub protein, hSAS-6, to the outer microtubules, and suggest that this interaction stabilizes the proper cartwheel structure for further CPAP-mediated centriole elongation. PMID: 23511974
  6. A truncating mutation of CEP135 caused autosomal-recessive primary microcephaly in a Pakistani family. PMID: 22521416
  7. CEP135 acts as a platform protein for C-NAP1 at the centriole. PMID: 18851962

Show More

Hide All

Involvement in disease
Microcephaly 8, primary, autosomal recessive (MCPH8)
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Note=During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
Protein Families
CEP135/TSGA10 family
Database Links

HGNC: 29086

OMIM: 611423

KEGG: hsa:9662

STRING: 9606.ENSP00000257287

UniGene: Hs.518767

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*