CNGB1 Antibody

Datasheet

Code	CSB-PA623897LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 kDa Observed band size: 140 kDa IHC image of CSB-PA623897LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA623897LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CNGB1 Polyclonal antibody

Uniprot No.

Q14028

Target Names

CNGB1

Alternative Names

CNGB1 antibody; CNCG2 antibody; CNCG3L antibody; CNCG4 antibody; RCNC2Cyclic nucleotide-gated cation channel beta-1 antibody; Cyclic nucleotide-gated cation channel 4 antibody; CNG channel 4 antibody; CNG-4 antibody; CNG4 antibody; Cyclic nucleotide-gated cation channel gamma antibody; Cyclic nucleotide-gated cation channel modulatory subunit antibody; Cyclic nucleotide-gated channel beta-1 antibody; CNG channel beta-1 antibody; Glutamic acid-rich protein antibody; GARP antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Cyclic nucleotide-gated cation channel beta-1 protein (131-253AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CNGB1 Antibody (Product code: CSB-PA623897LA01HU) is Non-conjugated. For CNGB1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA623897LB01HU	CNGB1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA623897LC01HU	CNGB1 Antibody, FITC conjugated
Biotin	CSB-PA623897LD01HU	CNGB1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP.; Isoform GARP2 is a high affinity rod photoreceptor phosphodiesterase (PDE6)-binding protein that modulates its catalytic properties: it is a regulator of spontaneous activation of rod PDE6, thereby serving to lower rod photoreceptor 'dark noise' and allowing these sensory cells to operate at the single photon detection limit.

Gene References into Functions

Here, we present the first case of RP45 caused by an unequivocal homozygous nonsense variant of CNGB1 identified by run of homozygosity (ROH) analysis. PMID: 26901671
Helios, and not FoxP3, is the marker of activated Tregs expressing GARP/LAP, and FoxP3+Helios+ Tregs have more suppressive characteristics, compared with FoxP3+Helios- Tregs. PMID: 26343373
GARP2 expression in cones can be detrimental to cones. RDS/GARP interactions remain under investigation but are critical for both OS structure and function. PMID: 26720471
The p.Arg86Gln mutation actually appears to be a polymorphism common in ethnic West Africans and not associated with RP. This change may provide a useful marker for West African ancestry. PMID: 23201897
Deletion of the cyclic nucleotide gated channel CNGB1 alters response waveform but leaves light adaptation intact in a transgenic model. PMID: 21123569
When expressed in a heterologous expression system the corresponding mutant full-length CNGB1a subunit was more susceptible to proteosomal degradation compared to the wild-type counterpart PMID: 20126465
we report targeting of cyclic nucleotide-gated channel(CNG) to rod outer segment required interaction with ankyrin-G; ankyrin-G localized to rod outer segments, coimmunoprecipitated with CNG channel & bound to C-terminal domain of channel beta1 subunit PMID: 19299621

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Involvement in disease

Retinitis pigmentosa 45 (RP45)

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

Cyclic nucleotide-gated cation channel (TC 1.A.1.5) family, CNGB1 subfamily

Database Links

HGNC: 2151

OMIM: 600724

KEGG: hsa:1258

STRING: 9606.ENSP00000251102

UniGene: Hs.147062

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

cAMP signaling pathway cGMP-PKG signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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