CSNK2A3 Antibody

Datasheet

Code	CSB-PA086427LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysate All lanes: CSNK2A3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa IHC image of CSB-PA086427LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunoprecipitating CSNK2A3 in Hela whole cell lysate Lane 1: Rabbit control IgG instead of CSB-PA086427LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000) Lane 2: CSB-PA086427LA01HU (5µg) + Hela whole cell lysate (0.5mg) Lane 3: Hela whole cell lysate (20µg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CSNK2A3 Polyclonal antibody

Uniprot No.

Q8NEV1

Target Names

CSNK2A3

Alternative Names

CSNK2A3 antibody; CSNK2A1PCasein kinase II subunit alpha 3 antibody; CK II alpha 3 antibody; EC 2.7.11.1 antibody; Casein kinase II alpha 1 polypeptide pseudogene antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Casein kinase II subunit alpha 3 protein (151-391AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CSNK2A3 Antibody (Product code: CSB-PA086427LA01HU) is Non-conjugated. For CSNK2A3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA086427LB01HU	CSNK2A3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA086427LC01HU	CSNK2A3 Antibody, FITC conjugated
Biotin	CSB-PA086427LD01HU	CSNK2A3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down-regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression.

Gene References into Functions

Observational study of gene-disease association. (HuGE Navigator) PMID: 20625391

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily

Tissue Specificity

Detected in blood platelets and megakaryocyte cell lines. Poorly expressed in lung. Highly expressed in lung tumor tissues.

Database Links

HGNC: 2458

KEGG: hsa:283106

UniGene: Hs.644056

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Wnt signaling pathway NF-kappa B signaling pathway Mitophagy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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