CSNK2A3 Antibody

Code CSB-PA086427LA01HU
Size US$166
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Image
  • Western Blot
    Positive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysate
    All lanes: CSNK2A3 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • IHC image of CSB-PA086427LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating CSNK2A3 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA086427LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
    Lane 2: CSB-PA086427LA01HU (5µg) + Hela whole cell lysate (0.5mg)
    Lane 3: Hela whole cell lysate (20µg)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) CSNK2A3 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
CSNK2A3 antibody; CSNK2A1PCasein kinase II subunit alpha 3 antibody; CK II alpha 3 antibody; EC 2.7.11.1 antibody; Casein kinase II alpha 1 polypeptide pseudogene antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Casein kinase II subunit alpha 3 protein (151-391AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The CSNK2A3 Antibody (Product code: CSB-PA086427LA01HU) is Non-conjugated. For CSNK2A3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA086427LB01HU CSNK2A3 Antibody, HRP conjugated ELISA
FITC CSB-PA086427LC01HU CSNK2A3 Antibody, FITC conjugated
Biotin CSB-PA086427LD01HU CSNK2A3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:200-1:500
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down-regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression.
Gene References into Functions
  1. Observational study of gene-disease association. (HuGE Navigator) PMID: 20625391
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
Tissue Specificity
Detected in blood platelets and megakaryocyte cell lines. Poorly expressed in lung. Highly expressed in lung tumor tissues.
Database Links

HGNC: 2458

KEGG: hsa:283106

UniGene: Hs.644056

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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