CSNK2A3 Antibody

Western Blot
Positive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysate
All lanes: CSNK2A3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa

IHC image of CSB-PA086427LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating CSNK2A3 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA086427LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
Lane 2: CSB-PA086427LA01HU (5µg) + Hela whole cell lysate (0.5mg)
Lane 3: Hela whole cell lysate (20µg)