CSNK2A3 Antibody

Code CSB-PA086427LA01HU
Size US$299
Image
  • Western Blot
    Positive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysate
    All lanes: CSNK2A3 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • IHC image of CSB-PA086427LA01HU diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating CSNK2A3 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA086427LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
    Lane 2: CSB-PA086427LA01HU (5µg) + Hela whole cell lysate (0.5mg)
    Lane 3: Hela whole cell lysate (20µg)

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) CSNK2A3 Polyclonal antibody
Uniprot No. Q8NEV1
Target Names CSNK2A3
Alternative Names CSNK2A3 antibody; CSNK2A1PCasein kinase II subunit alpha 3 antibody; CK II alpha 3 antibody; EC 2.7.11.1 antibody; Casein kinase II alpha 1 polypeptide pseudogene antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Casein kinase II subunit alpha 3 protein (151-391AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The CSNK2A3 Antibody (Product code: CSB-PA086427LA01HU) is Non-conjugated. For CSNK2A3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA086427LB01HU CSNK2A3 Antibody, HRP conjugated ELISA
FITC CSB-PA086427LC01HU CSNK2A3 Antibody, FITC conjugated
Biotin CSB-PA086427LD01HU CSNK2A3 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:200-1:500
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down-regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression.
Gene References into Functions
  1. Observational study of gene-disease association. (HuGE Navigator) PMID: 20625391
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, CK2 subfamily
Tissue Specificity Detected in blood platelets and megakaryocyte cell lines. Poorly expressed in lung. Highly expressed in lung tumor tissues.
Database Links

HGNC: 2458

KEGG: hsa:283106

UniGene: Hs.644056

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Address
7505 Fannin St Ste 610-322 Houston, TX 77054, USA
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