DAPP1 Antibody

Datasheet

Code	CSB-PA892478LA01HU
Size	US$166
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Image	IHC image of CSB-PA892478LA01HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A549 cells with CSB-PA892478LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DAPP1 Polyclonal antibody

Uniprot No.

Q9UN19

Target Names

DAPP1

Alternative Names

Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (hDAPP1) (B lymphocyte adapter protein Bam32) (B-cell adapter molecule of 32 kDa), DAPP1, BAM32

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide protein (1-280aa)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DAPP1 Antibody (Product code: CSB-PA892478LA01HU) is Non-conjugated. For DAPP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA892478LB01HU	DAPP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA892478LC01HU	DAPP1 Antibody, FITC conjugated
Biotin	CSB-PA892478LD01HU	DAPP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:100-1:500
IF	1:100-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

Gene References into Functions

we propose a role for Bam32 in the signaling of MHC class I molecules in professional Ag-presenting DC for the regulation of CD8(+) T cell activation. PMID: 21930970
novel phosphorylation-dependent function of Bam32 in regulating Rac1 activation and actin remodeling. PMID: 15247305
BAM32 works downstream of Src family protein tyrosine kinases and functions to link B cell antigen receptor signals with antigen-processing pathways in B cells. PMID: 15494510

Subcellular Location

Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation.

Tissue Specificity

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.

Database Links

HGNC: 16500

OMIM: 605768

KEGG: hsa:27071

STRING: 9606.ENSP00000423602

UniGene: Hs.436271

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Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

B cell receptor signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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