DAPP1 Antibody

Code CSB-PA892478LA01HU
Size US$166
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Image
  • IHC image of CSB-PA892478LA01HU diluted at 1:300 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
  • Immunofluorescence staining of A549 cells with CSB-PA892478LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) DAPP1 Polyclonal antibody
Uniprot No.
Target Names
DAPP1
Alternative Names
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (hDAPP1) (B lymphocyte adapter protein Bam32) (B-cell adapter molecule of 32 kDa), DAPP1, BAM32
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide protein (1-280aa)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The DAPP1 Antibody (Product code: CSB-PA892478LA01HU) is Non-conjugated. For DAPP1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA892478LB01HU DAPP1 Antibody, HRP conjugated ELISA
FITC CSB-PA892478LC01HU DAPP1 Antibody, FITC conjugated
Biotin CSB-PA892478LD01HU DAPP1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:100-1:500
IF 1:100-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
Gene References into Functions
  1. we propose a role for Bam32 in the signaling of MHC class I molecules in professional Ag-presenting DC for the regulation of CD8(+) T cell activation. PMID: 21930970
  2. novel phosphorylation-dependent function of Bam32 in regulating Rac1 activation and actin remodeling. PMID: 15247305
  3. BAM32 works downstream of Src family protein tyrosine kinases and functions to link B cell antigen receptor signals with antigen-processing pathways in B cells. PMID: 15494510
Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation.
Tissue Specificity
Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
Database Links

HGNC: 16500

OMIM: 605768

KEGG: hsa:27071

STRING: 9606.ENSP00000423602

UniGene: Hs.436271

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