DUSP9 Antibody

Datasheet

Code	CSB-PA859528LA01HU
Size	US$166
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Image	IHC image of CSB-PA859528LA01HU diluted at 1:600 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA859528LA01HU diluted at 1:600 and staining in paraffin-embedded human bladder cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA859528LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DUSP9 Polyclonal antibody

Uniprot No.

Q99956

Target Names

DUSP9

Alternative Names

Dual specificity phosphatase 9 antibody; Dual specificity protein phosphatase 9 antibody; DUS9_HUMAN antibody; DUSP9 antibody; MAP kinase phosphatase 4 antibody; Mitogen activated protein kinase phosphatase 4 antibody; Mitogen-activated protein kinase phosphatase 4 antibody; MKP 4 antibody; MKP-4 antibody; MKP4 antibody; serine/threonine specific protein phosphatase antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Dual specificity protein phosphatase 9 protein (142-263AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DUSP9 Antibody (Product code: CSB-PA859528LA01HU) is Non-conjugated. For DUSP9 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA859528LB01HU	DUSP9 Antibody, HRP conjugated	ELISA
FITC	CSB-PA859528LC01HU	DUSP9 Antibody, FITC conjugated
Biotin	CSB-PA859528LD01HU	DUSP9 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Inactivates MAP kinases. Has a specificity for the ERK family.

Gene References into Functions

The results of this study, combined with previous studies, suggested that therapeutic intervention to increase the expression or activity of DUSP9 may enable the activation of anti-proliferation signals in malignant cells. PMID: 25998184
A possible use for DUSP9 as CIMP marker. PMID: 24838152
DUSP9 protein levels were markedly suppressed in severe pre-eclampsia, but not in severe IUGR. This suppression might be attributable to the prolonged hypoxic conditions found in pre-eclampsia. PMID: 23276385
The DUSP9 locus is a common susceptibility locus for type 2 diabetes across different ethnicities, and 6 loci identified in South Asian genome-wide association studies also have significant effect on susceptibility to Japanese type 2 diabetes. PMID: 23029454
decreased expression of DUSP-9 is associated with poor prognosis in ccRCC. PMID: 21943117
2.7 A resolution crystal structure of the catalytic domain of MKP-4 (MKP-4C) is presented PMID: 21206059
microtubule disruption by MKP4 provides a novel mechanism for tumor suppression by a cytosolic MKP PMID: 18006813

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Subcellular Location

Cytoplasm.

Protein Families

Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily

Database Links

HGNC: 3076

OMIM: 300134

KEGG: hsa:1852

STRING: 9606.ENSP00000345853

UniGene: Hs.144879

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

MAPK signaling pathway Signaling pathways regulating pluripotency of stem cells

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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