EDC4 Antibody

Datasheet

Code	CSB-PA744034LA01HU
Size	US$166
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Image	IHC image of CSB-PA744034LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA744034LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA744034LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EDC4 Polyclonal antibody

Uniprot No.

Q6P2E9

Target Names

EDC4

Alternative Names

autoantigen antibody; Autoantigen Ge 1 antibody; Autoantigen Ge-1 antibody; Autoantigen Ge1 antibody; Autoantigen RCD 8 antibody; Autoantigen RCD-8 antibody; Autoantigen RCD8 antibody; edc4 antibody; EDC4_HUMAN antibody; Enhancer of mRNA decapping 4 antibody; Enhancer of mRNA-decapping protein 4 antibody; Ge 1 antibody; Hedls antibody; Human enhancer of decapping large subunit antibody; RCD 8 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Enhancer of mRNA-decapping protein 4 protein (753-952AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EDC4 Antibody (Product code: CSB-PA744034LA01HU) is Non-conjugated. For EDC4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA744034LB01HU	EDC4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA744034LC01HU	EDC4 Antibody, FITC conjugated
Biotin	CSB-PA744034LD01HU	EDC4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Gene References into Functions

EDC4 plays a key role in homologous recombination by stimulating end resection at double-strand breaks. PMID: 29511213
The assembly of EDC4 and Dcp1a into processing bodies is critical for the translational regulation of IL-6. PMID: 25970328
EDC4 is a processing body (P-body) component. PMID: 24858563
The data indicates that DCP2 activation by DCP1 occurs preferentially on the EDC4 scaffold, which may serve to couple DCP2 activation by DCP1 with 5'-to-3' mRNA degradation by XRN1 in human cells. PMID: 24510189
EDC4 might contribute to regulation of CoA biosynthesis in addition to its scaffold function in processing bodies PMID: 22982864

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Subcellular Location

Cytoplasm, P-body. Nucleus.

Protein Families

WD repeat EDC4 family

Database Links

HGNC: 17157

OMIM: 606030

KEGG: hsa:23644

STRING: 9606.ENSP00000351811

UniGene: Hs.75682

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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