EFHD1 Antibody

Datasheet

Code	CSB-PA880107LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate All lanes: EFHD1 antibody at 3.4μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27, 17 kDa Observed band size: 27 kDa IHC image of CSB-PA880107LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA880107LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Latest Promotion
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EFHD1 Polyclonal antibody

Uniprot No.

Q9BUP0

Target Names

EFHD1

Alternative Names

4931430I01Rik antibody; AI452351 antibody; DKFZp781H0842; antibody; EF hand domain containing 1 antibody; EF hand domain family; member D1 antibody; EF-hand domain-containing protein 1 antibody; EF-hand domain-containing protein D1 antibody; EFHD1 antibody; EFHD1_HUMAN antibody; FLJ13612 antibody; MGC103094 antibody; Mitocalcin antibody; MST133 antibody; MSTP133 antibody; PP3051 antibody; RGD1559565 antibody; Swiprosin 2 antibody; Swiprosin-2 antibody; SWS2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human EF-hand domain-containing protein D1 protein (15-72AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EFHD1 Antibody (Product code: CSB-PA880107LA01HU) is Non-conjugated. For EFHD1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA880107LB01HU	EFHD1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA880107LC01HU	EFHD1 Antibody, FITC conjugated
Biotin	CSB-PA880107LD01HU	EFHD1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production. May play a role in neuronal differentiation.

Gene References into Functions

EFHD1 functions as a novel mitochondrial Ca(2+) sensor underlying Ca(2+)-dependent activation of mitoflashes PMID: 26975899
detection of methylation of PPP1R3C alone or in combination with EFHD1 in plasma DNA showed high sensitivity and specificity in CRC detection, and may be useful detection method for CRC, especially for early-stage CRCs. PMID: 24861485
Cloning of novel mouse genes associated with neuronal function, including Efhd1. PMID: 12270117

Subcellular Location

Mitochondrion inner membrane.

Database Links

HGNC: 29556

OMIM: 611617

KEGG: hsa:80303

STRING: 9606.ENSP00000264059

UniGene: Hs.516769

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address