EIF3K Antibody

Code CSB-PA866204ESR1HU
Size US$166
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  • Western blot
    All lanes: EIF3K antibody at 1.72µg/ml
    Lane 1: THP-1 whole cell lysate
    Lane 2: HT29 whole cell lysate
    Lane 3: 293T whole cell lysate
    Lane 4: Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 26, 25 kDa
    Observed band size: 26 kDa

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA866204ESR1HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA866204ESR1HU at dilution of 1:100

  • Immunoprecipitating EIF3K in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA866204ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
    Lane 2: CSB-PA866204ESR1HU (4µg) + Hela whole cell lysate (500µg)
    Lane 3: Hela whole cell lysate (20µg)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) EIF3K Polyclonal antibody
Uniprot No.
Target Names
EIF3K
Alternative Names
ARG134 antibody; eIF 3 p25 antibody; eIF 3 p28 antibody; eIF-3 p25 antibody; eIF-3 p28 antibody; EIF3-p28 antibody; eif3k antibody; EIF3K_HUMAN antibody; EIF3S12 antibody; Eukaryotic translation initiation factor 3 subunit 12 antibody; Eukaryotic translation initiation factor 3 subunit K antibody; HSPC029 antibody; M9 antibody; MSTP001 antibody; Muscle specific gene M9 protein antibody; Muscle-specific gene M9 protein antibody; PLAC 24 antibody; PLAC-24 antibody; PLAC24 antibody; PRO1474 antibody; PTD001 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Eukaryotic translation initiation factor 3 subunit K protein (1-218AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 1 reviews

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Applications : WB

Sample type: Human Cell

Review: Western blots for EIF3 indiceted a clear co-migration with the 40S subunit, implying biologically relevant interactions and differentiation of distinct cellular complexes.

By Anonymous

Target Background

Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
Gene References into Functions
  1. eIF3K subunit interacts directly with the PML protein. PMID: 24036361
  2. identification of a novel 24-kDa protein that binds directly to dynein intermediate chain [PLAC-24] PMID: 12006665
  3. eIF3k has three putative protein-binding surfaces and has potential RNA binding activity PMID: 15180986
  4. cyclin D3 specifically interacted with eIF3k through its C-terminal domain; eIF3k distributed both in nucleus and cytoplasm and colocalized with cyclin D3 PMID: 15327989
  5. eIF3k, originally identified as the smallest subunit of eukaryotic translation initiation factor 3 (eIF3) complexes, also localizes to keratin intermediate filaments and physically associates with K18 in epithelial cells. PMID: 18577580

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
EIF-3 subunit K family
Tissue Specificity
Ubiquitous, with the highest levels of expression in brain, testis and kidney.
Database Links

HGNC: 24656

OMIM: 609596

KEGG: hsa:27335

STRING: 9606.ENSP00000248342

UniGene: Hs.314359

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