EIF3K Antibody

Datasheet

Citations (1)

Code	CSB-PA866204ESR1HU
Size	US$166
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Image	Western blot All lanes: EIF3K antibody at 1.72µg/ml Lane 1: THP-1 whole cell lysate Lane 2: HT29 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 26, 25 kDa Observed band size: 26 kDa Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA866204ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA866204ESR1HU at dilution of 1:100 Immunoprecipitating EIF3K in Hela whole cell lysate Lane 1: Rabbit control IgG instead of (1µg) instead of CSB-PA866204ESR1HU in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA866204ESR1HU (4µg) + Hela whole cell lysate (500µg) Lane 3: Hela whole cell lysate (20µg)
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EIF3K Polyclonal antibody

Uniprot No.

Q9UBQ5

Target Names

EIF3K

Alternative Names

ARG134 antibody; eIF 3 p25 antibody; eIF 3 p28 antibody; eIF-3 p25 antibody; eIF-3 p28 antibody; EIF3-p28 antibody; eif3k antibody; EIF3K_HUMAN antibody; EIF3S12 antibody; Eukaryotic translation initiation factor 3 subunit 12 antibody; Eukaryotic translation initiation factor 3 subunit K antibody; HSPC029 antibody; M9 antibody; MSTP001 antibody; Muscle specific gene M9 protein antibody; Muscle-specific gene M9 protein antibody; PLAC 24 antibody; PLAC-24 antibody; PLAC24 antibody; PRO1474 antibody; PTD001 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Eukaryotic translation initiation factor 3 subunit K protein (1-218AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

The non-coding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumour cells EP Booy,J Biol Chem,2020

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : WB

Sample type: Human Cell

Review: Western blots for EIF3 indiceted a clear co-migration with the 40S subunit, implying biologically relevant interactions and differentiation of distinct cellular complexes.

By Anonymous

Target Background

Function

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Gene References into Functions

eIF3K subunit interacts directly with the PML protein. PMID: 24036361
identification of a novel 24-kDa protein that binds directly to dynein intermediate chain [PLAC-24] PMID: 12006665
eIF3k has three putative protein-binding surfaces and has potential RNA binding activity PMID: 15180986
cyclin D3 specifically interacted with eIF3k through its C-terminal domain; eIF3k distributed both in nucleus and cytoplasm and colocalized with cyclin D3 PMID: 15327989
eIF3k, originally identified as the smallest subunit of eukaryotic translation initiation factor 3 (eIF3) complexes, also localizes to keratin intermediate filaments and physically associates with K18 in epithelial cells. PMID: 18577580

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

EIF-3 subunit K family

Tissue Specificity

Ubiquitous, with the highest levels of expression in brain, testis and kidney.

Database Links

HGNC: 24656

OMIM: 609596

KEGG: hsa:27335

STRING: 9606.ENSP00000248342

UniGene: Hs.314359

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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