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Rabbits were immunized with the recombinant human Endomucin protein (19-135aa) to produce the EMCN polyclonal antibodies. This stimulates the rabbit's immune system to produce a varied set of antibodies that recognize distinct epitopes on the antigen. Subsequently, the antibodies were purified from the rabbits' sera using antigen affinity chromatography.
The effectiveness of the EMCN antibody was evaluated in ELISA and IHC applications, and it was found to efficiently identify and bind the EMCN protein in human samples. The EMCN protein is involved in controlling vascular permeability and angiogenesis, which is the process of forming new blood vessels.
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