Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EPHB1/EPHB2/EPHB3 Polyclonal antibody

Uniprot No.

P54762

Target Names

EPHB1/EPHB2/EPHB3

Alternative Names

Cek 6 antibody; EK6 antibody; ELK antibody; Elkh antibody; EPH receptor B1 antibody; Eph tyrosine kinase 2 antibody; EPH-like kinase 6 antibody; Ephb1 antibody; EPHB1_HUMAN antibody; Ephrin type B receptor 1 antibody; Ephrin type-B receptor 1 antibody; EPHT2 antibody; HEK 6 antibody; HEK6 antibody; NET antibody; Neuronally-expressed EPH-related tyrosine kinase antibody; soluble EPHB1 variant 1 antibody; Tyrosine protein kinase receptor EPH 2 antibody; Tyrosine-protein kinase receptor EPH-2 antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthesized peptide derived from internal of Human EPHB1/2/3.

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Tested Applications

ELISA,IHC,IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:100
IF	1:100-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation.

Gene References into Functions

SUMOylation of EphB1 repressed activation of its downstream signaling molecule PKC-gamma, and consequently inhibited neuroblastoma tumorigenesis. PMID: 29550816
investigate NET could modulate one's attention orientation to facial expressions, we categorized individuals according to the genotypes of the -182 T/C (rs2242446) polymorphism. Our results indicated that the -182 T/C polymorphism significantly modulated attention orientation to facial expressions, of which the CC genotype facilitated attention reorientation to the locations where cued faces were previously presented. PMID: 27541794
some of the mutations found in EPHB1 may contribute to an increased invasive capacity of cancers. PMID: 28108514
Association of EPHB1 rs11918092 with symptoms of schizophrenia in Chinese Zhuang and Han populations. PMID: 27028544
The tumor-suppressor function of EphB1 is clinically relevant across many malignancies, suggesting that EphB1 is an important regulator of common cancer cell transforming pathways. PMID: 25944917
In medulloblastoma cell lines, EphB1 downregulation or knockdown reduced cell growth, viability, cell-cycle regulator expression, and migration, but increased radiosensitivity and the percentage of cells in G1 phase of the cell cycle. PMID: 25879388
Our results indicate that EphB1 may be involved in carcinogenesis of renal cell carcinoma PMID: 25120806
The genes CD248, Ephb1 and P2RY2 were detected as the top overexpressed in GC biopsies. PMID: 24716914
The study presents the first structure of the EphB1 tyrosine kinase domain determined by X-ray crystallography to 2.5A. PMID: 24677421
EphB1 and Ephrin-B could be regarded as independent good prognostic factors and important biological markers for Squamous cell/adenosquamous carcinoma and adenocarcinoma of gallbladder. PMID: 24606480
Our data indicate that loss of EphB1 protein is associated with metastasis and poorer survival in patients with serous ovarian cancer PMID: 24427352
Low EphB1 expression is associated with glioma. PMID: 24121831
EphB1 stimulation triggered approximately 50% serine-threonine PTEN dephosphorylation and PTEN-Cbl complex disruption, a process requiring PTEN protein phosphatase activity. PMID: 23118026
No association is found for EPH receptor B1 and susceptibility to schizophrenia. PMID: 21041834
EPHB1 polymorphisms may be associated with susceptibility to hepatocellular carcinoma in the Korean population. PMID: 21763378
Data show that EphB receptors interact with E-cadherin and with the metalloproteinase ADAM10 at sites of adhesion. PMID: 21804545
analysis of EphB1, EphB2, and EphB4-binding peptides interaction with antagonists with ephrin-like affinity PMID: 15722342
The ubiquitin ligase Cbl induces the ubiquitination and lysosomal degradation of activated EphB1, a process requiring EphB1 and Src kinase activity. PMID: 18034775
Transgenic EphB1 and ephrin-B3 cooperatively regulate the proliferation and migration of neural progenitors in the hippocampus. PMID: 18057206
Loss of expression of EphB1 protein in gastric carcinoma is associated with invasion and metastasis PMID: 18424888
EphB1 may have roles in the pathogenesis and development of colorectal cancer. PMID: 18931529

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Early endosome membrane. Cell projection, dendrite.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily

Tissue Specificity

Preferentially expressed in brain.

Database Links

HGNC: 3392

OMIM: 600600

KEGG: hsa:2047

STRING: 9606.ENSP00000381097

UniGene: Hs.116092

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Code	CSB-PA106334
Size	US$297
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Image	Immunohistochemistry analysis of paraffin-embedded human brain tissue using EPHB1/2/3 antibody. Immunofluorescence analysis of NIH/3T3 cells, using EPHB1/2/3 antibody.
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EPHB1/EPHB2/EPHB3 Antibody

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